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Q55247

- GLNB_SYNY3

UniProt

Q55247 - GLNB_SYNY3

Protein

Nitrogen regulatory protein P-II

Gene

glnB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is phosphorylated, these events are reversed. In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is phosphorylated which allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme By similarity.By similarity

    GO - Molecular functioni

    1. enzyme regulator activity Source: InterPro
    2. nucleotide binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of nitrogen utilization Source: InterPro
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrogen regulatory protein P-II
    Alternative name(s):
    PII signal transducing protein
    Gene namesi
    Name:glnB
    Ordered Locus Names:ssl0707
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 112112Nitrogen regulatory protein P-IIPRO_0000139795Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei49 – 491PhosphoserineCurated
    Modified residuei51 – 511O-UMP-tyrosinePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylation dependent on the nitrogen source and spectral light quality.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ55247.

    PTM databases

    PhosSiteiP0806321.

    Interactioni

    Subunit structurei

    Homotrimer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    sll0985P731485EBI-906365,EBI-906297

    Protein-protein interaction databases

    IntActiQ55247. 3 interactions.
    STRINGi1148.ssl0707.

    Structurei

    Secondary structure

    1
    112
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 87
    Helixi10 – 123
    Helixi13 – 219
    Turni22 – 243
    Beta strandi28 – 358
    Beta strandi56 – 6510
    Helixi67 – 693
    Helixi70 – 8112
    Beta strandi90 – 956
    Beta strandi98 – 1014
    Turni102 – 1043
    Beta strandi107 – 1093

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UL3X-ray2.00A/B/C/D1-112[»]
    ProteinModelPortaliQ55247.
    SMRiQ55247. Positions 1-112.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ55247.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the P(II) protein family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0347.
    HOGENOMiHOG000017847.
    KOiK04751.
    OMAiVCEVERV.
    PhylomeDBiQ55247.

    Family and domain databases

    Gene3Di3.30.70.120. 1 hit.
    InterProiIPR002187. N-reg_PII.
    IPR011322. N-reg_PII-like_a/b.
    IPR015867. N-reg_PII/ATP_PRibTrfase_C.
    IPR017918. N-reg_PII_CS.
    IPR002332. N-reg_PII_urydylation_site.
    [Graphical view]
    PfamiPF00543. P-II. 1 hit.
    [Graphical view]
    PRINTSiPR00340. PIIGLNB.
    SMARTiSM00938. P-II. 1 hit.
    [Graphical view]
    SUPFAMiSSF54913. SSF54913. 1 hit.
    PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
    PS51343. PII_GLNB_DOM. 1 hit.
    PS00496. PII_GLNB_UMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q55247-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKVEAIIRP FKLDEVKIAL VNAGIVGMTV SEVRGFGRQK GQTERYRGSE    50
    YTVEFLQKLK IEIVVDEGQV DMVVDKLVSA ARTGEIGDGK IFISPVDSVV 100
    RIRTGEKDTE AI 112
    Length:112
    Mass (Da):12,397
    Last modified:November 1, 1997 - v1
    Checksum:iF9ABD0F5C173B799
    GO

    Sequence cautioni

    The sequence BAA18533.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97496 Genomic DNA. Translation: CAA66127.1.
    BA000022 Genomic DNA. Translation: BAA18533.1. Different initiation.
    PIRiS76404.
    RefSeqiNP_441855.2. NC_000911.1.
    YP_005651915.1. NC_017277.1.
    YP_007451736.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA18533; BAA18533; BAA18533.
    GeneIDi952897.
    KEGGisyn:ssl0707.
    syy:SYNGTS_1962.
    syz:MYO_119810.
    PATRICi23841230. VBISynSp132158_2165.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97496 Genomic DNA. Translation: CAA66127.1 .
    BA000022 Genomic DNA. Translation: BAA18533.1 . Different initiation.
    PIRi S76404.
    RefSeqi NP_441855.2. NC_000911.1.
    YP_005651915.1. NC_017277.1.
    YP_007451736.1. NC_020286.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UL3 X-ray 2.00 A/B/C/D 1-112 [» ]
    ProteinModelPortali Q55247.
    SMRi Q55247. Positions 1-112.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q55247. 3 interactions.
    STRINGi 1148.ssl0707.

    PTM databases

    PhosSitei P0806321.

    Proteomic databases

    PaxDbi Q55247.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA18533 ; BAA18533 ; BAA18533 .
    GeneIDi 952897.
    KEGGi syn:ssl0707.
    syy:SYNGTS_1962.
    syz:MYO_119810.
    PATRICi 23841230. VBISynSp132158_2165.

    Phylogenomic databases

    eggNOGi COG0347.
    HOGENOMi HOG000017847.
    KOi K04751.
    OMAi VCEVERV.
    PhylomeDBi Q55247.

    Miscellaneous databases

    EvolutionaryTracei Q55247.

    Family and domain databases

    Gene3Di 3.30.70.120. 1 hit.
    InterProi IPR002187. N-reg_PII.
    IPR011322. N-reg_PII-like_a/b.
    IPR015867. N-reg_PII/ATP_PRibTrfase_C.
    IPR017918. N-reg_PII_CS.
    IPR002332. N-reg_PII_urydylation_site.
    [Graphical view ]
    Pfami PF00543. P-II. 1 hit.
    [Graphical view ]
    PRINTSi PR00340. PIIGLNB.
    SMARTi SM00938. P-II. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54913. SSF54913. 1 hit.
    PROSITEi PS00638. PII_GLNB_CTER. 1 hit.
    PS51343. PII_GLNB_DOM. 1 hit.
    PS00496. PII_GLNB_UMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nitrogen availability and electron transport control the expression of glnB gene (encoding PII protein) in the cyanobacterium Synechocystis sp. PCC 6803."
      Garcia-Dominguez M., Florencio F.J.
      Plant Mol. Biol. 35:723-734(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.
    3. "The structures of the PII proteins from the cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803."
      Xu Y., Carr P.D., Clancy P., Garcia-Dominguez M., Forchhammer K., Florencio F., Vasudevan S.G., Tandeau de Marsac N., Ollis D.L.
      Acta Crystallogr. D 59:2183-2190(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiGLNB_SYNY3
    AccessioniPrimary (citable) accession number: Q55247
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3