Q55247 (GLNB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogen regulatory protein P-II

Alternative name(s):
PII signal transducing protein

Gene names

Name:	glnB
Ordered Locus Names:	ssl0707

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is phosphorylated, these events are reversed. In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is phosphorylated which allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme By similarity.
Subunit structure	Homotrimer By similarity.
Post-translational modification	Phosphorylation dependent on the nitrogen source and spectral light quality By similarity.
Sequence similarities	Belongs to the P(II) protein family.
Sequence caution	The sequence BAA18533.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	Nucleotide-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of catalytic activity Inferred from electronic annotation. Source: GOC regulation of nitrogen utilization Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	enzyme regulator activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
sll0985	P73148	5	EBI-906365,EBI-906297

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 112

112

Nitrogen regulatory protein P-II

PRO_0000139795

Amino acid modifications

Modified residue

49

1

Phosphoserine Probable

Modified residue

51

1

O-UMP-tyrosine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

112

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Helix

10 – 12

3

Helix

13 – 21

9

Turn

22 – 24

3

Beta strand

28 – 35

8

Beta strand

56 – 65

10

Helix

67 – 69

3

Helix

70 – 81

12

Beta strand

90 – 95

6

Beta strand

98 – 101

4

Turn

102 – 104

3

Beta strand

107 – 109

3

Sequences

	Sequence		Length	Mass (Da)
	Q55247 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F9ABD0F5C173B799	FASTA	112	12,397
	10 20 30 40 50 60 MKKVEAIIRP FKLDEVKIAL VNAGIVGMTV SEVRGFGRQK GQTERYRGSE YTVEFLQKLK 70 80 90 100 110 IEIVVDEGQV DMVVDKLVSA ARTGEIGDGK IFISPVDSVV RIRTGEKDTE AI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nitrogen availability and electron transport control the expression of glnB gene (encoding PII protein) in the cyanobacterium Synechocystis sp. PCC 6803." Garcia-Dominguez M., Florencio F.J. Plant Mol. Biol. 35:723-734(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.
[3]	"The structures of the PII proteins from the cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803." Xu Y., Carr P.D., Clancy P., Garcia-Dominguez M., Forchhammer K., Florencio F., Vasudevan S.G., Tandeau de Marsac N., Ollis D.L. Acta Crystallogr. D 59:2183-2190(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X97496 Genomic DNA. Translation: CAA66127.1.
BA000022 Genomic DNA. Translation: BAA18533.1. Different initiation.

PIR

S76404.

RefSeq

NP_441855.2. NC_000911.1.
YP_005651915.1. NC_017277.1.
YP_007451736.1. NC_020286.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UL3	X-ray	2.00	A/B/C/D	1-112	[»]

ProteinModelPortal

Q55247.

SMR

Q55247. Positions 1-112.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q55247. 3 interactions.

STRING

1148.ssl0707.

PTM databases

PhosSite

P0806321.

Proteomic databases

PaxDb

Q55247.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAA18533; BAA18533; BAA18533.

GeneID

12254574.
14617403.
952897.

KEGG

syn:ssl0707.
syy:SYNGTS_1962.

PATRIC

23841230. VBISynSp132158_2165.

Phylogenomic databases

eggNOG

COG0347.

HOGENOM

HOG000017847.

KO

K04751.

OMA

GFIAMTI.

ProtClustDB

CLSK895726.

Family and domain databases

Gene3D

3.30.70.120. 1 hit.

InterPro

IPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]

Pfam

PF00543. P-II. 1 hit.
[Graphical view]

PRINTS

PR00340. PIIGLNB.

SMART

SM00938. P-II. 1 hit.
[Graphical view]

SUPFAM

SSF54913. N-reg_PII-like_a/b. 1 hit.

PROSITE

PS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q55247.

Entry information

Entry name

GLNB_SYNY3

Accession

Primary (citable) accession number: Q55247

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program