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Q55247 (GLNB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrogen regulatory protein P-II
Alternative name(s):
PII signal transducing protein
Gene names
Name:glnB
Ordered Locus Names:ssl0707
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length112 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is phosphorylated, these events are reversed. In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is phosphorylated which allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme By similarity.

Subunit structure

Homotrimer By similarity.

Post-translational modification

Phosphorylation dependent on the nitrogen source and spectral light quality By similarity.

Sequence similarities

Belongs to the P(II) protein family.

Sequence caution

The sequence BAA18533.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

sll0985P731485EBI-906365,EBI-906297

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 112112Nitrogen regulatory protein P-II
PRO_0000139795

Amino acid modifications

Modified residue491Phosphoserine Probable
Modified residue511O-UMP-tyrosine By similarity

Secondary structure

..................... 112
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q55247 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F9ABD0F5C173B799

FASTA11212,397
        10         20         30         40         50         60 
MKKVEAIIRP FKLDEVKIAL VNAGIVGMTV SEVRGFGRQK GQTERYRGSE YTVEFLQKLK 

        70         80         90        100        110 
IEIVVDEGQV DMVVDKLVSA ARTGEIGDGK IFISPVDSVV RIRTGEKDTE AI 

« Hide

References

« Hide 'large scale' references
[1]"Nitrogen availability and electron transport control the expression of glnB gene (encoding PII protein) in the cyanobacterium Synechocystis sp. PCC 6803."
Garcia-Dominguez M., Florencio F.J.
Plant Mol. Biol. 35:723-734(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.
[3]"The structures of the PII proteins from the cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803."
Xu Y., Carr P.D., Clancy P., Garcia-Dominguez M., Forchhammer K., Florencio F., Vasudevan S.G., Tandeau de Marsac N., Ollis D.L.
Acta Crystallogr. D 59:2183-2190(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97496 Genomic DNA. Translation: CAA66127.1.
BA000022 Genomic DNA. Translation: BAA18533.1. Different initiation.
PIRS76404.
RefSeqNP_441855.2. NC_000911.1.
YP_005651915.1. NC_017277.1.
YP_007451736.1. NC_020286.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UL3X-ray2.00A/B/C/D1-112[»]
ProteinModelPortalQ55247.
SMRQ55247. Positions 1-112.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ55247. 3 interactions.
STRING1148.ssl0707.

PTM databases

PhosSiteP0806321.

Proteomic databases

PaxDbQ55247.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18533; BAA18533; BAA18533.
GeneID12254574.
14617403.
952897.
KEGGsyn:ssl0707.
syy:SYNGTS_1962.
syz:MYO_119810.
PATRIC23841230. VBISynSp132158_2165.

Phylogenomic databases

eggNOGCOG0347.
HOGENOMHOG000017847.
KOK04751.
OMAIFVTEVE.
PhylomeDBQ55247.
ProtClustDBCLSK895726.

Family and domain databases

Gene3D3.30.70.120. 1 hit.
InterProIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
IPR002332. N-reg_PII_urydylation_site.
[Graphical view]
PfamPF00543. P-II. 1 hit.
[Graphical view]
PRINTSPR00340. PIIGLNB.
SMARTSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMSSF54913. SSF54913. 1 hit.
PROSITEPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
PS00496. PII_GLNB_UMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ55247.

Entry information

Entry nameGLNB_SYNY3
AccessionPrimary (citable) accession number: Q55247
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references