Q551X9 (DHKF_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hybrid signal transduction histidine kinase F
EC=2.7.13.3

Gene names

Name:	dhkF
ORF Names:	DDB_G0276143

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1109 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a receptor histidine kinase for a signal transduction pathway. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transducer Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1109	1109	Hybrid signal transduction histidine kinase F		PRO_0000375998
Regions
	Domain	237 – 289	53	PAC
	Domain	324 – 547	224	Histidine kinase
	Domain	928 – 1048	121	Response regulatory
	Coiled coil	719 – 760	42	Potential
	Compositional bias	715 – 719	5	Poly-Ser
	Compositional bias	731 – 758	28	Poly-Gln
	Compositional bias	1052 – 1099	48	Poly-Asn
Amino acid modifications
	Modified residue	327	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	977	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	54	1	K → KVC in AAK54087. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q551X9 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 42A2586314902434	FASTA	1,109	125,421
	10 20 30 40 50 60 MILKFIIQQC VFKVAKIFSD NLIQIFMDIS SVYSSSTLFF SKLSLLMCKV FNSKCAGISV 70 80 90 100 110 120 LNKDGLFDTI AFCSSTQELE NFSYSLKKIS KNITYYDNIN EIFNNSEIIK KYNLKSYMEI 130 140 150 160 170 180 PLKDSKEFYF GDIVLFGDQT LNPNILDTNI LTFITNRTSM EFEKKKVSDQ LIIAKNLLDQ 190 200 210 220 230 240 SPSCSCLVSR NGKIIRKIGA FYERLLGLEI GENISSVEKS SDISTILKQV CFENNNLSTE 250 260 270 280 290 300 TTITKKNGEK YPAEVFVKEI SDIHSNSIGI MIIVRDITDQ IRLKEMNIEL QKKSELEQKR 310 320 330 340 350 360 NQELMEARDL ALTATKIKSQ FLATISHEIR TPLNGIITMG EMLLSTSPLN TEQHDIAETI 370 380 390 400 410 420 FGSSELLLSI TSDILDFSKI EASKLELEMI EFDFIGCLEG IGKTIGVSIT NKPIEIAFLM 430 440 450 460 470 480 DTDIPHRLIG DPNRLIQIML NMGTNAVKYT DRGHIVFRIS VISREQNRCK IKISIDDSGI 490 500 510 520 530 540 GIGEDQRAHL FEPFHQIDSS STRKYGGSGL GLAISSKLAK LMGGEVILER SKPGVGSLFS 550 560 570 580 590 600 VTLNFEQIGS NTLKSLLPDK SFSRNKTCII LDNYEYTASI ASQRFDQIFE ESNIILVKES 610 620 630 640 650 660 TVKKFFELVQ EYKINGDVNK IDLKNLDPEL LPFFDSSLIC IIVFHRFMDN LDIFLKYVKD 670 680 690 700 710 720 FIEFYKKKIV VALGINHKNF KNLPKRRDFL IFKKPISSTN LIKVSNMARK IIPKSSSSSN 730 740 750 760 770 780 LIQTISQIDN QQQQQQQQLQ QQEQEQQHQQ QQLQQEQQFV TISPHSDSSE KKTTPKKDRG 790 800 810 820 830 840 KYDFNISPLR IDRFGTESTS SPKIKLYSDT SSDSGESDEF EFSENLRELK SDGELVVLKN 850 860 870 880 890 900 QPYKEQRLSN IQLINDPIVT PPTPKANSEV VSTKAESTFF QSSSITIKET RSDSVNSVNS 910 920 930 940 950 960 VNSVNSVNSL NSENSNNSLD VGIRTRPRIL LVDDNAVNRK VVKLQLKKLG YDCDTATNGF 970 980 990 1000 1010 1020 EGFEMQKKDN YELIFMDLNM PLCDGSMASK LIRSHEELNN YKSRANIVGL SATYLHGSKD 1030 1040 1050 1060 1070 1080 YCVSMGMDDF VVKPLKLQPL GELVKKYLEV ENNNNNNNNN NNNNNNNNSN NNNSNSNSNP 1090 1100 NSNSNSNSNS NSNPNQNPNY CNNLPTDFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The histidine kinases of Dictyostelium." Anjard C., Loomis W.F. (In) Inouye M., Dutta R. (eds.); Histidine kinases in signal transduction, pp.1-1, Academic press, San Diego (2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX4.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF362368 Genomic DNA. Translation: AAK54087.2. AAFI02000014 Genomic DNA. Translation: EAL69371.1.
RefSeq	XP_643349.1. XM_638257.1.
3D structure databases
HSSP	HSSP built from PDB template 1OXK based on UniProtKB P39928.
ProteinModelPortal	Q551X9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185098; DDB0185098; DDB_G0276143.
GeneID	8620399.
GenomeReviews	Gene locus dhkF in contig CM000151_GR.
KEGG	ddi:DDB_G0276143.
Organism-specific databases
dictyBase	DDB_G0276143. dhkF.
Phylogenomic databases
eggNOG	KOG0519.
GeneTree	EPrGT00050000000304.
InParanoid	Q551X9.
ProtClustDB	CLSZ2846868.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011006. CheY-like_superfamily. IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00086. PAC. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF52172. CheY_like. 1 hit. SSF47384. His_kin_homodim. 1 hit.
TIGRFAMs	TIGR00229. Sensory_box. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. PS50113. PAC. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHKF_DICDI

Accession

Primary (citable) accession number: Q551X9
Secondary accession number(s): Q86IA0, Q95PI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)