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Protein

Phytochrome-like protein cph1

Gene

cph1

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region. Has also a slight blue shift for the far-red maximum. Forms a two-component system with the rcp1 response regulator.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei259Tetrapyrrole chromophore (covalent; via 1 link)By similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: UniProtKB
  • red or far-red light photoreceptor activity Source: UniProtKB

GO - Biological processi

  • detection of visible light Source: InterPro
  • protein autophosphorylation Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB
  • red, far-red light phototransduction Source: UniProtKB
  • regulation of transcription, DNA-templated Source: InterPro
  • response to red or far red light Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Photoreceptor protein, Receptor, Transferase

Keywords - Biological processi

Sensory transduction

Keywords - Ligandi

ATP-binding, Chromophore, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.13.3. 382.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytochrome-like protein cph1 (EC:2.7.13.3)
Alternative name(s):
Bacteriophytochrome cph1
Light-regulated histidine kinase 1
Gene namesi
Name:cph1
Ordered Locus Names:slr0473
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi538H → K: No autophosphorylation; no phosphotransfer to Rcp1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001720011 – 748Phytochrome-like protein cph1Add BLAST748

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei538Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Contains one covalently linked tetrapyrrole chromophore.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ55168.

PTM databases

iPTMnetiQ55168.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-594457,EBI-594457
rcp1Q551692EBI-594457,EBI-766949

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-34651N.
IntActiQ55168. 10 interactors.
MINTiMINT-8375044.

Structurei

Secondary structure

1748
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 16Combined sources8
Helixi20 – 22Combined sources3
Beta strandi30 – 36Combined sources7
Turni37 – 40Combined sources4
Beta strandi41 – 46Combined sources6
Helixi49 – 53Combined sources5
Helixi57 – 60Combined sources4
Helixi65 – 68Combined sources4
Beta strandi70 – 73Combined sources4
Helixi83 – 88Combined sources6
Beta strandi90 – 98Combined sources9
Turni99 – 101Combined sources3
Beta strandi102 – 112Combined sources11
Beta strandi118 – 124Combined sources7
Helixi136 – 149Combined sources14
Helixi153 – 168Combined sources16
Beta strandi171 – 178Combined sources8
Beta strandi184 – 191Combined sources8
Beta strandi201 – 203Combined sources3
Helixi205 – 207Combined sources3
Helixi210 – 218Combined sources9
Beta strandi221 – 225Combined sources5
Beta strandi227 – 229Combined sources3
Beta strandi232 – 238Combined sources7
Turni240 – 242Combined sources3
Beta strandi252 – 254Combined sources3
Helixi258 – 266Combined sources9
Beta strandi270 – 279Combined sources10
Beta strandi282 – 293Combined sources12
Helixi299 – 344Combined sources46
Beta strandi345 – 347Combined sources3
Helixi348 – 353Combined sources6
Helixi356 – 361Combined sources6
Turni362 – 364Combined sources3
Beta strandi366 – 372Combined sources7
Beta strandi375 – 381Combined sources7
Helixi385 – 397Combined sources13
Beta strandi404 – 407Combined sources4
Helixi409 – 411Combined sources3
Helixi414 – 419Combined sources6
Helixi420 – 423Combined sources4
Beta strandi424 – 431Combined sources8
Turni432 – 434Combined sources3
Beta strandi435 – 441Combined sources7
Beta strandi446 – 452Combined sources7
Helixi454 – 456Combined sources3
Beta strandi458 – 463Combined sources6
Beta strandi466 – 470Combined sources5
Beta strandi478 – 482Combined sources5
Helixi491 – 514Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VEAX-ray2.21A1-514[»]
3ZQ5X-ray1.95A1-514[»]
ProteinModelPortaliQ55168.
SMRiQ55168.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ55168.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 86PASAdd BLAST68
Domaini152 – 320GAFAdd BLAST169
Domaini535 – 748Histidine kinasePROSITE-ProRule annotationAdd BLAST214

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 510Chromophore binding domainAdd BLAST424

Sequence similaritiesi

In the N-terminal section; belongs to the phytochrome family.Curated
Contains 1 GAF domain.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Phylogenomic databases

HOGENOMiHOG000030537.
InParanoidiQ55168.
KOiK11354.
OMAiCAQEPIQ.
PhylomeDBiQ55168.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013654. PAS_2.
IPR016132. Phyto_chromo_attachment.
IPR001294. Phytochrome.
IPR013515. Phytochrome_cen-reg.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF08446. PAS_2. 1 hit.
PF00360. PHY. 1 hit.
[Graphical view]
PRINTSiPR01033. PHYTOCHROME.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55781. SSF55781. 2 hits.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50046. PHYTOCHROME_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTVQLSDQ SLRQLETLAI HTAHLIQPHG LVVVLQEPDL TISQISANCT
60 70 80 90 100
GILGRSPEDL LGRTLGEVFD SFQIDPIQSR LTAGQISSLN PSKLWARVMG
110 120 130 140 150
DDFVIFDGVF HRNSDGLLVC ELEPAYTSDN LPFLGFYHMA NAALNRLRQQ
160 170 180 190 200
ANLRDFYDVI VEEVRRMTGF DRVMLYRFDE NNHGDVIAED KRDDMEPYLG
210 220 230 240 250
LHYPESDIPQ PARRLFIHNP IRVIPDVYGV AVPLTPAVNP STNRAVDLTE
260 270 280 290 300
SILRSAYHCH LTYLKNMGVG ASLTISLIKD GHLWGLIACH HQTPKVIPFE
310 320 330 340 350
LRKACEFFGR VVFSNISAQE DTETFDYRVQ LAEHEAVLLD KMTTAADFVE
360 370 380 390 400
GLTNHPDRLL GLTGSQGAAI CFGEKLILVG ETPDEKAVQY LLQWLENREV
410 420 430 440 450
QDVFFTSSLS QIYPDAVNFK SVASGLLAIP IARHNFLLWF RPEVLQTVNW
460 470 480 490 500
GGDPNHAYEA TQEDGKIELH PRQSFDLWKE IVRLQSLPWQ SVEIQSALAL
510 520 530 540 550
KKAIVNLILR QAEELAQLAR NLERSNADLK KFAYIASHDL QEPLNQVSNY
560 570 580 590 600
VQLLEMRYSE ALDEDAKDFI DFAVTGVSLM QTLIDDILTY AKVDTQYAQL
610 620 630 640 650
TFTDVQEVVD KALANLKQRI EESGAEIEVG SMPAVMADQI QLMQVFQNLI
660 670 680 690 700
ANGIKFAGDK SPKIKIWGDR QEDAWVFAVQ DNGIGIDPQF FERIFVIFQR
710 720 730 740
LHTRDEYKGT GMGLAICKKI IEGHQGQIWL ESNPGEGSTF YFSIPIGN
Length:748
Mass (Da):84,233
Last modified:November 1, 1996 - v1
Checksum:iA9ECA6D8D5B3C88A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10307.1.
PIRiS74389.

Genome annotation databases

EnsemblBacteriaiBAA10307; BAA10307; BAA10307.
KEGGisyn:slr0473.
PATRICi23842112. VBISynSp132158_2597.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA10307.1.
PIRiS74389.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VEAX-ray2.21A1-514[»]
3ZQ5X-ray1.95A1-514[»]
ProteinModelPortaliQ55168.
SMRiQ55168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-34651N.
IntActiQ55168. 10 interactors.
MINTiMINT-8375044.

PTM databases

iPTMnetiQ55168.

Proteomic databases

PRIDEiQ55168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10307; BAA10307; BAA10307.
KEGGisyn:slr0473.
PATRICi23842112. VBISynSp132158_2597.

Phylogenomic databases

HOGENOMiHOG000030537.
InParanoidiQ55168.
KOiK11354.
OMAiCAQEPIQ.
PhylomeDBiQ55168.

Enzyme and pathway databases

BRENDAi2.7.13.3. 382.

Miscellaneous databases

EvolutionaryTraceiQ55168.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013654. PAS_2.
IPR016132. Phyto_chromo_attachment.
IPR001294. Phytochrome.
IPR013515. Phytochrome_cen-reg.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF08446. PAS_2. 1 hit.
PF00360. PHY. 1 hit.
[Graphical view]
PRINTSiPR01033. PHYTOCHROME.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55781. SSF55781. 2 hits.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50046. PHYTOCHROME_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHY1_SYNY3
AccessioniPrimary (citable) accession number: Q55168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The R form exhibits both ATP-dependent autophosphorylation and phosphotransfer to rcp1 activities. Unlike the higher plants where Pfr is thought to be the active form.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.