Q55135 (PURU_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formyltetrahydrofolate deformylase
EC=3.5.1.10

Alternative name(s):
Formyl-FH(4) hydrolase

Gene names

Name:	purU
Ordered Locus Names:	sll0070

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
Sequence similarities	Belongs to the purU family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	One-carbon metabolism Purine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro formyltetrahydrofolate deformylase activity Inferred from electronic annotation. Source: EC hydroxymethyl-, formyl- and related transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Formyltetrahydrofolate deformylase		PRO_0000074967
Regions
	Domain	6 – 84	79	ACT
Sites
	Active site	228	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55135 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D9C0ADC38AE537C9	FASTA	284	32,634
	10 20 30 40 50 60 MQNLTATLLV SCPDQPGIVA QIAQFIYQNQ GNIIHADQHT DFSSGLFLNR VEWQLDNFRL 70 80 90 100 110 120 SRPELLSAWS QLAEQLQATW QIHFSDQLPR LALWVSKQDH CLLDILWRWR SGELRCEIPL 130 140 150 160 170 180 IISNHPDLKS IADQFGIDFH CLPITKENKL AQETAELALL KQYQIDLVVL AKYLQILTTD 190 200 210 220 230 240 FVVQFPNIIN IHHSFLPAFP GANPYHRAHE RGVKIIGATA HYATAQLDEG PIIEQDVVRV 250 260 270 280 SHRDNVDDLI RKGRDLERVV LARAVRLHLQ HRILVYDNRT VVFA

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10270.1.
PIR	S74352.
RefSeq	NP_442200.1. NC_000911.1.
3D structure databases
ProteinModelPortal	Q55135.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55135. 4 interactions.
STRING	Q55135.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952266.
GenomeReviews	Gene locus sll0070 in contig BA000022_GR.
KEGG	syn:sll0070.
NMPDR	fig|1148.1.peg.2301.
PATRIC	23842022. VBISynSp132158_2553.
Phylogenomic databases
eggNOG	COG0788.
HOGENOM	HBG286016.
OMA	IRIDHSD.
PhylomeDB	Q55135.
ProtClustDB	PRK06027.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0070-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR002376. Formyl_transf_N. IPR004810. PurU. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K01433.
PANTHER	PTHR10520:SF7. PTHR10520:SF7. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
PIRSF	PIRSF036480. FormyFH4_hydr. 1 hit.
PRINTS	PR01575. FFH4HYDRLASE.
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00655. PurU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURU_SYNY3

Accession

Primary (citable) accession number: Q55135

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program