Reviewed, UniProtKB/Swiss-Prot Q55135 (PURU_SYNY3)

Names and origin

Protein names

Recommended name:
    Formyltetrahydrofolate deformylase
    EC=3.5.1.10
Alternative name(s):
    Formyl-FH(4) hydrolase

Gene names

Name:	purU
Ordered Locus Names:	sll0070

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
Sequence similarities	Belongs to the purU family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	One-carbon metabolism Purine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro formyltetrahydrofolate deformylase activity Inferred from electronic annotation. Source: EC hydroxymethyl-, formyl- and related transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Formyltetrahydrofolate deformylase		PRO_0000074967
Regions
	Domain	6 – 84	79	ACT
Sites
	Active site	228	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55135-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D9C0ADC38AE537C9	FASTA	284	32,634
	10 20 30 40 50 60 MQNLTATLLV SCPDQPGIVA QIAQFIYQNQ GNIIHADQHT DFSSGLFLNR VEWQLDNFRL 70 80 90 100 110 120 SRPELLSAWS QLAEQLQATW QIHFSDQLPR LALWVSKQDH CLLDILWRWR SGELRCEIPL 130 140 150 160 170 180 IISNHPDLKS IADQFGIDFH CLPITKENKL AQETAELALL KQYQIDLVVL AKYLQILTTD 190 200 210 220 230 240 FVVQFPNIIN IHHSFLPAFP GANPYHRAHE RGVKIIGATA HYATAQLDEG PIIEQDVVRV 250 260 270 280 SHRDNVDDLI RKGRDLERVV LARAVRLHLQ HRILVYDNRT VVFA

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10270.1.
PIR	S74352.
RefSeq	NP_442200.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q55135. 4 interactions.
STRING	Q55135.
Genome annotation databases
GeneID	952266.
GenomeReviews	Gene locus sll0070 in contig BA000022_GR.
KEGG	syn:sll0070.
NMPDR	fig|1148.1.peg.2301.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q55135.
OMA	EGHCLND
Enzyme and pathway databases
BioCyc	SSP1148:SLL0070-MON.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR002376. Formyl_transf_N. IPR004810. PurU. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
PIRSF	PIRSF036480. FormyFH4_hydr. 1 hit.
PRINTS	PR01575. FFH4HYDRLASE.
TIGRFAMs	TIGR00655. PurU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURU_SYNY3

Accession

Primary (citable) accession number: Q55135

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)