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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei27 – 271NucleophileUniRule annotation
Metal bindingi268 – 2681Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi315 – 3151MagnesiumUniRule annotation
Metal bindingi377 – 3771MagnesiumUniRule annotation
Metal bindingi378 – 3781MagnesiumUniRule annotation
Metal bindingi414 – 4141Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi465 – 4651Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi468 – 4681Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_0004-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:Synpcc7942_0004
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000002717 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2626By similarityPRO_0000029271Add
BLAST
Chaini27 – 493467AmidophosphoribosyltransferasePRO_0000029272Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi1140.Synpcc7942_0004.

Structurei

3D structure databases

ProteinModelPortaliQ55038.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 252226Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q55038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPTQPLTAD LDCDLGLERP DRPEEACGVF ALYAPGEEVA RMAYFGLYAL
60 70 80 90 100
QHRGQESAGI AVFEGDRVML HKDMGLVSQV FDPEILQQLQ GSLAVGHTRY
110 120 130 140 150
STTGSSRIAN AQPALLETRL GPVALAHNGN LVNTVELRQE LLAKNHELTT
160 170 180 190 200
TTDSELIAFA IMEAVAEGQD WRGAIESACR RSQGAFSLTI GTPEALYGTR
210 220 230 240 250
DPNGIRPLVL GTLESNGQTR YVLSSETCGL DIIGADYVRD IAPGEMVRIT
260 270 280 290 300
DAGLESWTWA EAPQPKLCVF EMIYFARPDS LFHGESLYSY RRRIGQRLAK
310 320 330 340 350
EAPADVDLVL GVPDSGIPAA IGFSEALGIP YAEGLIKNRY VGRTFIQPTQ
360 370 380 390 400
SMRETGIRMK LNPLKDVLAG KRIAIIDDSI VRGTTSRKLV KALRDAGATE
410 420 430 440 450
VHMRISSPPV THPCFYGIDT DTQDQLIAAT RSVSEITEQI GVDSLAYLTE
460 470 480 490
QGMLEATRES IGNFCTACFN GRYPIAIPEE IKRSKLMLET VTA
Length:493
Mass (Da):53,809
Last modified:November 1, 1996 - v1
Checksum:iE03508F8A922910C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33211 Genomic DNA. Translation: AAA75107.1.
CP000100 Genomic DNA. Translation: ABB56036.1.
PIRiS77612.
RefSeqiWP_011243803.1. NC_007604.1.
YP_399023.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB56036; ABB56036; Synpcc7942_0004.
KEGGisyf:Synpcc7942_0004.
PATRICi23785029. VBISynElo51371_0065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33211 Genomic DNA. Translation: AAA75107.1.
CP000100 Genomic DNA. Translation: ABB56036.1.
PIRiS77612.
RefSeqiWP_011243803.1. NC_007604.1.
YP_399023.1. NC_007604.1.

3D structure databases

ProteinModelPortaliQ55038.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1140.Synpcc7942_0004.

Protein family/group databases

MEROPSiC44.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB56036; ABB56036; Synpcc7942_0004.
KEGGisyf:Synpcc7942_0004.
PATRICi23785029. VBISynElo51371_0065.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciSYNEL:SYNPCC7942_0004-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Circadian expression of genes involved in the purine biosynthetic pathway of the cyanobacterium Synechococcus sp. strain PCC 7942."
    Liu Y., Tsinoremas N.F., Golden S.S., Kondo T., Johnson C.H.
    Mol. Microbiol. 20:1071-1081(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7942.

Entry informationi

Entry nameiPUR1_SYNE7
AccessioniPrimary (citable) accession number: Q55038
Secondary accession number(s): Q31SD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.