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Q55038 (PUR1_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:Synpcc7942_0004
OrganismSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]
Taxonomic identifier1140 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 2626 By similarity
PRO_0000029271
Chain27 – 493467Amidophosphoribosyltransferase
PRO_0000029272

Regions

Domain27 – 252226Glutamine amidotransferase type-2

Sites

Active site271For GATase activity By similarity
Metal binding2681Iron-sulfur (4Fe-4S) By similarity
Metal binding3151Magnesium By similarity
Metal binding3771Magnesium By similarity
Metal binding3781Magnesium By similarity
Metal binding4141Iron-sulfur (4Fe-4S) By similarity
Metal binding4651Iron-sulfur (4Fe-4S) By similarity
Metal binding4681Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q55038 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E03508F8A922910C

FASTA49353,809
        10         20         30         40         50         60 
MIPTQPLTAD LDCDLGLERP DRPEEACGVF ALYAPGEEVA RMAYFGLYAL QHRGQESAGI 

        70         80         90        100        110        120 
AVFEGDRVML HKDMGLVSQV FDPEILQQLQ GSLAVGHTRY STTGSSRIAN AQPALLETRL 

       130        140        150        160        170        180 
GPVALAHNGN LVNTVELRQE LLAKNHELTT TTDSELIAFA IMEAVAEGQD WRGAIESACR 

       190        200        210        220        230        240 
RSQGAFSLTI GTPEALYGTR DPNGIRPLVL GTLESNGQTR YVLSSETCGL DIIGADYVRD 

       250        260        270        280        290        300 
IAPGEMVRIT DAGLESWTWA EAPQPKLCVF EMIYFARPDS LFHGESLYSY RRRIGQRLAK 

       310        320        330        340        350        360 
EAPADVDLVL GVPDSGIPAA IGFSEALGIP YAEGLIKNRY VGRTFIQPTQ SMRETGIRMK 

       370        380        390        400        410        420 
LNPLKDVLAG KRIAIIDDSI VRGTTSRKLV KALRDAGATE VHMRISSPPV THPCFYGIDT 

       430        440        450        460        470        480 
DTQDQLIAAT RSVSEITEQI GVDSLAYLTE QGMLEATRES IGNFCTACFN GRYPIAIPEE 

       490 
IKRSKLMLET VTA 

« Hide

References

« Hide 'large scale' references
[1]"Circadian expression of genes involved in the purine biosynthetic pathway of the cyanobacterium Synechococcus sp. strain PCC 7942."
Liu Y., Tsinoremas N.F., Golden S.S., Kondo T., Johnson C.H.
Mol. Microbiol. 20:1071-1081(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7942.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33211 Genomic DNA. Translation: AAA75107.1.
CP000100 Genomic DNA. Translation: ABB56036.1.
PIRS77612.
RefSeqYP_399023.1. NC_007604.1.

3D structure databases

ProteinModelPortalQ55038.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1140.Synpcc7942_0004.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB56036; ABB56036; Synpcc7942_0004.
GeneID3774224.
KEGGsyf:Synpcc7942_0004.
PATRIC23785029. VBISynElo51371_0065.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
KOK00764.
OrthoDBEOG6KT2Q1.

Enzyme and pathway databases

BioCycSYNEL:SYNPCC7942_0004-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_SYNE7
AccessionPrimary (citable) accession number: Q55038
Secondary accession number(s): Q31SD3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways