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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei27NucleophileUniRule annotation1
Metal bindingi268Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi315MagnesiumUniRule annotation1
Metal bindingi377MagnesiumUniRule annotation1
Metal bindingi378MagnesiumUniRule annotation1
Metal bindingi414Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi465Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi468Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_0004-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:Synpcc7942_0004
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002717 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000292711 – 26By similarityAdd BLAST26
ChainiPRO_000002927227 – 493AmidophosphoribosyltransferaseAdd BLAST467

Proteomic databases

PRIDEiQ55038.

Interactioni

Protein-protein interaction databases

STRINGi1140.Synpcc7942_0004.

Structurei

3D structure databases

ProteinModelPortaliQ55038.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 252Glutamine amidotransferase type-2UniRule annotationAdd BLAST226

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033688.
KOiK00764.
OrthoDBiPOG091H0061.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q55038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPTQPLTAD LDCDLGLERP DRPEEACGVF ALYAPGEEVA RMAYFGLYAL
60 70 80 90 100
QHRGQESAGI AVFEGDRVML HKDMGLVSQV FDPEILQQLQ GSLAVGHTRY
110 120 130 140 150
STTGSSRIAN AQPALLETRL GPVALAHNGN LVNTVELRQE LLAKNHELTT
160 170 180 190 200
TTDSELIAFA IMEAVAEGQD WRGAIESACR RSQGAFSLTI GTPEALYGTR
210 220 230 240 250
DPNGIRPLVL GTLESNGQTR YVLSSETCGL DIIGADYVRD IAPGEMVRIT
260 270 280 290 300
DAGLESWTWA EAPQPKLCVF EMIYFARPDS LFHGESLYSY RRRIGQRLAK
310 320 330 340 350
EAPADVDLVL GVPDSGIPAA IGFSEALGIP YAEGLIKNRY VGRTFIQPTQ
360 370 380 390 400
SMRETGIRMK LNPLKDVLAG KRIAIIDDSI VRGTTSRKLV KALRDAGATE
410 420 430 440 450
VHMRISSPPV THPCFYGIDT DTQDQLIAAT RSVSEITEQI GVDSLAYLTE
460 470 480 490
QGMLEATRES IGNFCTACFN GRYPIAIPEE IKRSKLMLET VTA
Length:493
Mass (Da):53,809
Last modified:November 1, 1996 - v1
Checksum:iE03508F8A922910C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33211 Genomic DNA. Translation: AAA75107.1.
CP000100 Genomic DNA. Translation: ABB56036.1.
PIRiS77612.
RefSeqiWP_011243803.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB56036; ABB56036; Synpcc7942_0004.
KEGGisyf:Synpcc7942_0004.
PATRICi23785029. VBISynElo51371_0065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33211 Genomic DNA. Translation: AAA75107.1.
CP000100 Genomic DNA. Translation: ABB56036.1.
PIRiS77612.
RefSeqiWP_011243803.1. NC_007604.1.

3D structure databases

ProteinModelPortaliQ55038.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1140.Synpcc7942_0004.

Protein family/group databases

MEROPSiC44.A03.

Proteomic databases

PRIDEiQ55038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB56036; ABB56036; Synpcc7942_0004.
KEGGisyf:Synpcc7942_0004.
PATRICi23785029. VBISynElo51371_0065.

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033688.
KOiK00764.
OrthoDBiPOG091H0061.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciSYNEL:SYNPCC7942_0004-MONOMER.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR1_SYNE7
AccessioniPrimary (citable) accession number: Q55038
Secondary accession number(s): Q31SD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.