Q55013 (CY550_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c-550

Alternative name(s):
Cytochrome c549
Cytochrome c550
Low-potential cytochrome c

Gene names

Name:	psbV
Synonyms:	petK
Ordered Locus Names:	sll0258

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-potential cytochrome c that plays a role in the oxygen-evolving complex of photosystem II (PSII). Required for normal function or stabilization of PSII. Extrinsic protein associated with PSII that enhances oxygen evolution. HAMAP MF_01378
Cofactor	Binds 1 heme group covalently per subunit.
Subunit structure	The cyanobacterial oxygen-evolving complex is composed of psbO, psbP, psbQ, psbV and psbU Potential.
Subcellular location	Cellular thylakoid membrane; Peripheral membrane protein; Lumenal side Probable. Note: Associated with photosystem II at the lumenal side of the thylakoid membrane Probable. Ref.1
Disruption phenotype	Cells show decreased but not abolished level of PSII and photoautotrophic growth. Ref.1
Sequence similarities	Belongs to the cytochrome c family. PsbV subfamily.
Sequence caution	The sequence AAA19982.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Membrane Photosystem II Thylakoid
Domain	Signal
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW respiratory electron transport chain Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane-derived thylakoid photosystem II Inferred from direct assay. Source: UniProtKB thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Ref.1 Ref.3 Ref.4

Chain

26 – 160

135

Cytochrome c-550 HAMAP MF_01378

PRO_0000006521

Sites

Metal binding

66

1

Iron (heme axial ligand)

Metal binding

117

1

Iron (heme axial ligand)

Binding site

62

1

Heme (covalent)

Binding site

65

1

Heme (covalent)

Experimental info

Sequence conflict

60

1

D → T in AAA19982. Ref.3

Sequence conflict

71

1

Missing AA sequence Ref.1

Sequence conflict

90 – 91

2

RR → PS in AAA19982. Ref.3

Sequence conflict

94

1

V → R in AAA19982. Ref.3

Sequence conflict

138 – 139

2

FD → YN in AAA19982. Ref.3

Sequence conflict

142

1

G → A in AAA19982. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

160

Helix Strand Turn

Details...

Turn

30 – 33

4

Beta strand

34 – 41

8

Beta strand

43 – 45

3

Helix

48 – 61

14

Helix

63 – 66

4

Helix

67 – 69

3

Beta strand

71 – 76

6

Beta strand

78 – 80

3

Helix

81 – 85

5

Beta strand

87 – 89

3

Helix

94 – 102

9

Turn

114 – 116

3

Turn

123 – 125

3

Helix

127 – 129

3

Helix

134 – 150

17

Sequences

	Sequence		Length	Mass (Da)
	Q55013 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 3DA6F835DB79F665	FASTA	160	17,884
	10 20 30 40 50 60 MKRFFLVAIA SVLFFFNTMV GSANAVELTE STRTIPLDEA GGTTTLTARQ FTNGQKIFVD 70 80 90 100 110 120 TCTQCHLQGK TKTNNNVSLG LADLAGAEPR RDNVLALVEF LKNPKSYDGE DDYSELHPNI 130 140 150 160 SRPDIYPEMR NYTEDDIFDV AGYTLIAPKL DERWGGTIYF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The role of cytochrome c-550 as studied through reverse genetics and mutant characterization in Synechocystis sp. PCC 6803." Shen J.-R., Vermaas W., Inoue Y. J. Biol. Chem. 270:6901-6907(1995) [PubMed: 7896839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-73, PROBABLE SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[3]	"Cloning and sequence analysis of the gene encoding the low potential cytochrome c of Synechocystis PCC 6803." Kang C., Chitnis P.R., Smith S., Krogmann D.W. FEBS Lett. 344:5-9(1994) [PubMed: 8181563] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-160, PROTEIN SEQUENCE OF 26-49.
[4]	"Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: linking 130 protein spots with their respective genes." Sazuka T., Ohara O. Electrophoresis 18:1252-1258(1997) [PubMed: 9298645] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-40.
[5]	"Crystal structure of low-potential cytochrome c549 from Synechocystis sp. PCC 6803 at 1.21 A resolution." Frazao C., Enguita F.J., Coelho R., Sheldrick G.M., Navarro J.A., Hervas M., De la Rosa M.A., Carrondo M.A. J. Biol. Inorg. Chem. 6:324-332(2001) [PubMed: 11315568] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.21 ANGSTROMS) OF 26-160.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D45178 Genomic DNA. Translation: BAA08124.1.
BA000022 Genomic DNA. Translation: BAA18512.1.
U07021 Genomic DNA. Translation: AAA19982.2. Different initiation.

PIR

A56189.

RefSeq

NP_441834.1. NC_000911.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E29	X-ray	1.21	A	26-160	[»]

ProteinModelPortal

Q55013.

SMR

Q55013. Positions 26-160.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q55013. 1 interaction.

STRING

Q55013.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

954270.

GenomeReviews

Gene locus sll0258 in contig BA000022_GR.

KEGG

syn:sll0258.

NMPDR

fig|1148.1.peg.1935.

PATRIC

23841184. VBISynSp132158_2143.

Phylogenomic databases

eggNOG

NOG13404.

HOGENOM

HBG285698.

OMA

WGGGKIY.

PhylomeDB

Q55013.

ProtClustDB

PRK13619.

Enzyme and pathway databases

BioCyc

MetaCyc:PSBV-MONOMER.
SSP1148:SLL0258-MONOMER.

Family and domain databases

HAMAP

MF_01378. PSII_Cyt550.
[Tree]

InterPro

IPR009056. Cyt_c_dom.
IPR016003. PSII_cyt_c550.
IPR017851. PSII_PsbV_cyt_c550.
[Graphical view]

Gene3D

G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.

KO

K02720.

PIRSF

PIRSF005890. Phot_II_cyt_c550. 1 hit.

SUPFAM

SSF46626. Cytochrome_c. 1 hit.

TIGRFAMs

TIGR03045. PS_II_C550. 1 hit.

PROSITE

PS51007. CYTC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CY550_SYNY3

Accession

Primary (citable) accession number: Q55013
Secondary accession number(s): Q55333

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program