Q54ZY3 (SYCM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cysteine--tRNA ligase, mitochondrial
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	mcysS
ORF Names:	DDB_G0277295

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 583		Probable cysteine--tRNA ligase, mitochondrial		PRO_0000328522
Regions
	Motif	84 – 94	11	"HIGH" region
	Motif	337 – 341	5	"KMSKS" region
Sites
	Metal binding	82	1	Zinc By similarity
	Metal binding	271	1	Zinc By similarity
	Metal binding	296	1	Zinc By similarity
	Metal binding	300	1	Zinc By similarity
	Binding site	340	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54ZY3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: CF5B1AEA2F7AAD21	FASTA	583	67,503
	10 20 30 40 50 60 MLKLISRNAI NKNKIILFRL YTTTINSSNN GSTIREWKKP SLENSYDTGI MVKNSLFKNG 70 80 90 100 110 120 NVPFLISNCS EKNQRPISWY TCGPTVYSSS HIGHARNYMT VDIIQRILIN YFRFNIIHVM 130 140 150 160 170 180 GLTDIDDKII NKSKQELISA SELSKKFEQE FFEDLKSLNI KPPMFTTRVS EHIDEIIKYI 190 200 210 220 230 240 EKIKENQLTY ESTKSVIFDV NKFGIDRYCS LRAANQQIKS DDTLLEKDKK SSQDFVLWKA 250 260 270 280 290 300 YNENIDIDGT GNPVCWDSPF GKGRPGWHIE CSAMIDSIFK DHLDVHSGGV DLEFPHHQNE 310 320 330 340 350 360 IAQCEGHSHS HGSGDAESTQ WANYFFHIGH LILNNEKMSK SLGNVITIRD FLSRYSANSL 370 380 390 400 410 420 RWICLIHKYN DPLSFSDETL QLCISKEIKF LNWFKIVRSK LKLEQTSVNK VKKTFNHSIE 430 440 450 460 470 480 LLNQLSETKY LIEKDLKDDF NTPSVIKRLE LLMKQTNESM NSIDTDLLFN VQDYVESILN 490 500 510 520 530 540 IFGLEVNSEI SDHNKTDESN QFLLEKLLDF RSDVKNLAKN EKSLDQIKSK IYQITDQLRS 550 560 570 580 DCQSEISLRV SDIPDKGTNK PYTVDWLDKN HIQLLNIKKQ SKK

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000019 Genomic DNA. Translation: EAL68834.1.
RefSeq	XP_642733.1. XM_637641.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ProteinModelPortal	Q54ZY3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54ZY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231320; DDB0231320; DDB_G0277295.
GeneID	8620927.
GenomeReviews	Gene locus mcysS in contig CM000151_GR.
KEGG	ddi:DDB_G0277295.
Organism-specific databases
dictyBase	DDB_G0277295. mcysS.
Phylogenomic databases
eggNOG	KOG2007.
GeneTree	EPrGT00050000005734.
HOGENOM	HBG327651.
OMA	RESFRIR.
PhylomeDB	Q54ZY3.
ProtClustDB	PTZ00399.
Family and domain databases
InterPro	IPR015803. Cys-tRNA-synt. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCM_DICDI

Accession

Primary (citable) accession number: Q54ZY3
Secondary accession number(s): Q86AQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)