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Reviewed, UniProtKB/Swiss-Prot Q54ZY3 (SYCM_DICDI)

Last modified February 9, 2010. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable cysteinyl-tRNA synthetase, mitochondrial
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS
Gene names
Name: mcysS
ORF Names: DDB_G0277295
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

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Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcysteinyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 583Probable cysteinyl-tRNA synthetase, mitochondrialPRO_0000328522

Regions

Motif84 – 9411"HIGH" region
Motif337 – 3415"KMSKS" region

Sites

Metal binding821Zinc By similarity
Metal binding2711Zinc By similarity
Metal binding2961Zinc By similarity
Metal binding3001Zinc By similarity
Binding site3401ATP By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q54ZY3-1 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: CF5B1AEA2F7AAD21

FASTA58367,503
        10         20         30         40         50         60 
MLKLISRNAI NKNKIILFRL YTTTINSSNN GSTIREWKKP SLENSYDTGI MVKNSLFKNG 

        70         80         90        100        110        120 
NVPFLISNCS EKNQRPISWY TCGPTVYSSS HIGHARNYMT VDIIQRILIN YFRFNIIHVM 

       130        140        150        160        170        180 
GLTDIDDKII NKSKQELISA SELSKKFEQE FFEDLKSLNI KPPMFTTRVS EHIDEIIKYI 

       190        200        210        220        230        240 
EKIKENQLTY ESTKSVIFDV NKFGIDRYCS LRAANQQIKS DDTLLEKDKK SSQDFVLWKA 

       250        260        270        280        290        300 
YNENIDIDGT GNPVCWDSPF GKGRPGWHIE CSAMIDSIFK DHLDVHSGGV DLEFPHHQNE 

       310        320        330        340        350        360 
IAQCEGHSHS HGSGDAESTQ WANYFFHIGH LILNNEKMSK SLGNVITIRD FLSRYSANSL 

       370        380        390        400        410        420 
RWICLIHKYN DPLSFSDETL QLCISKEIKF LNWFKIVRSK LKLEQTSVNK VKKTFNHSIE 

       430        440        450        460        470        480 
LLNQLSETKY LIEKDLKDDF NTPSVIKRLE LLMKQTNESM NSIDTDLLFN VQDYVESILN 

       490        500        510        520        530        540 
IFGLEVNSEI SDHNKTDESN QFLLEKLLDF RSDVKNLAKN EKSLDQIKSK IYQITDQLRS 

       550        560        570        580 
DCQSEISLRV SDIPDKGTNK PYTVDWLDKN HIQLLNIKKQ SKK

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References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed: 12097910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFI02000019 Genomic DNA. Translation: EAL68834.1.
RefSeqXP_642733.1.

3D structure databases

HSSPHSSP built from PDB template 1LI5 based on UniProtKB P21888.
SMRQ54ZY3. Positions 78-460.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ54ZY3.

Genome annotation databases

GeneID8620927.
KEGGddi:DDB_0231320.

Organism-specific databases

dictyBaseDDB_G0277295. mcysS.

Phylogenomic databases

eggNOGKOG2007.
HOGENOMHBG327651.
OMASSSHIGH.
PhylomeDBQ54ZY3.

Enzyme and pathway databases

BRENDA6.1.1.16. 424.

Family and domain databases

InterProIPR015804. Cys-tRNA-synt_Ia_C.
IPR015803. Cys-tRNA-synt_Ia_N.
IPR002308. Cys-tRNA-synth_1a.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR10890. Cys_tRNA-synt_1a. 1 hit.
PfamPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
TIGRFAMsTIGR00435. cysS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameSYCM_DICDI
AccessionPrimary (citable) accession number: Q54ZY3
Secondary accession number(s): Q86AQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents