Reviewed, UniProtKB/Swiss-Prot Q54Z60 (ACSA_DICDI)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
ORF Names:	DDB_G0277815

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	Acetyl-coenzyme A synthetase		PRO_0000328354

Sequences

	Sequence		Length	Mass (Da)
	Q54Z60-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 4DF825F016ECAB8F	FASTA	674	75,586
	10 20 30 40 50 60 MQITEKETVQ EEQQKLLYPP PSFSTKCHIS SVEQYNEMYK ESIESPNQFW DKKAKEFLTW 70 80 90 100 110 120 FSDYTTVQHG SFEKGDISWF LNGKINVSYN CIDRHLKENA DKVAILFEGD EETMVKKVTY 130 140 150 160 170 180 REMFEEVCRL SNLLISLGVG KGDTVAIYLP NTPTAIYSML ACARIGAIHS VIFAGFGYES 190 200 210 220 230 240 IVSRVHDAKC RVIITADEGL RGGRYIPLKE KIDQVVQHCK LVQHVLVFKN TGRPTITFNP 250 260 270 280 290 300 SIDIWADEAM LDHRPYCPPV WLDSEDPLFI LYTSGSTGTP KGLVHTQAGY LLYAAMTHRY 310 320 330 340 350 360 VFDYHDSDVY ACMADVGWIT GHSYIVYGPL ANGATTFIFE GTPLYPTPAR YWEMVQRHKI 370 380 390 400 410 420 TQFYTAPTAI RSLMKFPISF TQQSDKSSLR VLGSVGEPIN PEAWRWFNTN VGEGRCAIVD 430 440 450 460 470 480 TYWQTESGGH LITPLPGVTS TKPGSATKPF FGIELQVLDS KTGERLYINP DINGCKEISG 490 500 510 520 530 540 VLAISKPWPG IARSVYRSHG RYLQTYMTQY KGHYFTGDGV KLDSDGYYWI EGRVDDVINV 550 560 570 580 590 600 SGHRLGTAEL ESALVGCSIC AEAAVVGYPH DIKGQGILAF CTLKEGYQED ESNVIMMLKK 610 620 630 640 650 660 EVRNVIGPFA TPDVIVITPS LPKTRSGKIM RRILRKIGCH ESSAEQLGDI STLAEPEVVK 670 LLIEKVSKVI PKTH

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000022 Genomic DNA. Translation: EAL68581.1.
RefSeq	XP_642493.1.
3D structure databases
HSSP	HSSP built from PDB template 1RY2 based on UniProtKB Q01574.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54Z60.
Genome annotation databases
GeneID	8621204.
KEGG	ddi:DDB_0231684.
Organism-specific databases
dictyBase	DDB_G0277815. acsA.
Phylogenomic databases
eggNOG	KOG1175.
HOGENOM	HBG547964.
OMA	IKDRVND.
PhylomeDB	Q54Z60.
Enzyme and pathway databases
BRENDA	6.2.1.1. 424.
Family and domain databases
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_DICDI

Accession

Primary (citable) accession number: Q54Z60
Secondary accession number(s): Q86AW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)