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Reviewed, UniProtKB/Swiss-Prot Q54Z25 (PLDC_DICDI)

Last modified September 22, 2009. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phospholipase D C
    EC=3.1.4.4
Alternative name(s):
    Phosphatase D1
      Short name=PLD 1
Gene names
Name: pldC
Synonyms: pld1
ORF Names: DDB_G0277949
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

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Protein attributes

Sequence length1640 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in cell growth. Hydrolyzes membrane phospholipids, such as PtdCho (phosphatidylcholine), producing the free headgroup and PtdOH (phosphatidic acid; signaling molecule on its own). Involved in the inhibition of actin-based motility and endocytosis. Its inhibition causes complete collapse of F-actin organization. Ref.2 Ref.3 Ref.5

Catalytic activity

A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulation

Inhibited by butan-1-ol. Ref.5

Sequence similarities

Belongs to the phospholipase D family.

Contains 2 PLD phosphodiesterase domains.

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Ontologies

Keywords
   Biological processLipid degradation
   DomainRepeat
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionNAPE-specific phospholipase D activity

Inferred from electronic annotation. Source: EC

phospholipase D activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16401640Phospholipase D C
PRO_0000367472

Regions

Domain1009 – 103628PLD phosphodiesterase 1
Domain1460 – 148728PLD phosphodiesterase 2
Compositional bias21 – 3313Poly-Gln
Compositional bias126 – 19368Asn-rich
Compositional bias149 – 1546Poly-Thr
Compositional bias201 – 21818Poly-Asp
Compositional bias277 – 2804Poly-His
Compositional bias327 – 3326Poly-Thr
Compositional bias395 – 40713Poly-Thr
Compositional bias467 – 4737Poly-Thr
Compositional bias474 – 49421Poly-Asn
Compositional bias1017 – 10204Poly-Val
Compositional bias1146 – 117631Poly-Asn
Compositional bias1188 – 11925Poly-Thr
Compositional bias1193 – 11986Poly-Asn
Compositional bias1207 – 12148Poly-Asn
Compositional bias1222 – 125231Poly-Asn
Compositional bias1253 – 126210Poly-Thr
Compositional bias1293 – 12975Poly-Gln
Compositional bias1298 – 13058Poly-His

Sites

Active site10141 Potential
Active site10161 Potential
Active site10211 Potential
Active site14651 Potential
Active site14671 Potential
Active site14721 Potential

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Sequences

Sequence LengthMass (Da)Tools
Q54Z25-1 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 58A9DF1E84EA6776

FASTA1,640188,250
        10         20         30         40         50         60 
MYFFTPPQKR TLKPVVDRVL QQQQQQQQQQ QQQSLPKFQP FEVETYSLED EKYLYNISYF 

        70         80         90        100        110        120 
SKEEIKLLYD LFHRINLDLD GFTENIIYQT LSFLVHIPSG IEELSSLFYN EDVIYRKEIN 

       130        140        150        160        170        180 
SSHRSNQSFN HSNSTTPLNT TNNNIKKPTT TTTTNSNNIN NNGNVNGNNT TNLDDILNNH 

       190        200        210        220        230        240 
NDNYSSDNSY LHNIYDDIYE DEDDEDDEDD DDDEDEDDEG KEFEQDDEDE STISSMSLKN 

       250        260        270        280        290        300 
SQAKRLSKKM NSIDLNVDEI SSTHNQNHQN HQNHQNHHHH TTTHMIEKKI TTTINKIKQK 

       310        320        330        340        350        360 
ISNESSGLLC EVVGAGGAGT EGASSSTTTT TTLSNDLQTN ESIKFVEDEK QQLKQQLRKQ 

       370        380        390        400        410        420 
LLKDGNHNSL NNILYDYKHP NDINTPIEEL TAAATTTTTT TSTTTTTNDH QNLEKTIISN 

       430        440        450        460        470        480 
KTIKKLEGVE TIKDTVELEK QQQKQQQTSN VTTAKVKDTI LSSSPKTTTT ATTNNNNNNN 

       490        500        510        520        530        540 
NNNNNNNNNN NNNNSYKYNS YLESSNISNI SNASYTDKPM DDEYYYGEYD DEDDSKPPSQ 

       550        560        570        580        590        600 
EKIESINLYR SQFSIIRDCN LWGGKTLKED EFDYIDSSSE ILKYRMIIKY MVENLFSYIR 

       610        620        630        640        650        660 
KRYNLEANKN PSIIHLIEII SIMTRGTLKE KSELVFKLVR KKSEGVVYKT ELLEMIQGID 

       670        680        690        700        710        720 
ALTVLNVFGL GSIGTPDEVV NNIYREGLST VNSIQRTPSF PRSDSFYQKS MSSEQPFFKD 

       730        740        750        760        770        780 
TSLEMKEFIK RSVSNSDIPR CFGFFDLIYL CYIKPIEDYL KSSIKYKQAS GYLYYEKYLG 

       790        800        810        820        830        840 
IIKAYSLRWF EVRSGFLIGY KRLFSKPSKV ICLFKTNVKI IPKEHPKHHM KLKSLFKGSL 

       850        860        870        880        890        900 
SKQIDGNKEA TDFVLRRFDD TEQTFISLSS HRASNFVNAI RENSKGSYRY HSFASPQEDI 

       910        920        930        940        950        960 
NVVPYINGST YFKGVYKALK HATSEIYIAG WWISPNVSLN RTATSKTPDK YRLDSVLMKK 

       970        980        990       1000       1010       1020 
ASEGVKIYIL IWDETMIAMD LGSRGVKSFF EKMHRRNIKV IRHPHMLPLY WSHHQKVVVV 

      1030       1040       1050       1060       1070       1080 
DQRIAFIGGL DLCFGRYDNE YYFVKDNLEI NFPGADYINS CIAKPVNNLK DCLVDRNTQP 

      1090       1100       1110       1120       1130       1140 
RMPWHDVSIS LDGKAARDVT YNFIQRWNHA KDSNRDYKSY PYLITSLETP LNIPHPQPPT 

      1150       1160       1170       1180       1190       1200 
QFLHPNTNIN NNNNNANNNT NNINNNNNNN NINNLNSSTN TTNNTNNTTT TTNNNNLNTS 

      1210       1220       1230       1240       1250       1260 
TNMLPINNNN NNLNTSTNIL PNNNNNNNNN NNNNNNNNNN NNNNNNNINN NNTTSTTTTT 

      1270       1280       1290       1300       1310       1320 
TTNNNLNSSS NNLNFNISGE IHNNSLPHQL NNQQQQQHHH HHHHHYQPPL PPQQRGTCKV 

      1330       1340       1350       1360       1370       1380 
QIVRSVCGWS AGQVLENSIY KAYLNLINLS QHFIYIQNQF FISSVGFTQP NNQIAFAIYK 

      1390       1400       1410       1420       1430       1440 
RIEKAVLLNQ VFRVILLLPV HCEGDIYDVD TQLIIKYTEK SITGIKTELL KKFPEMDIDQ 

      1450       1460       1470       1480       1490       1500 
YLSINSLRNW DANGDIIFTE QIYVHSKVLI VDDKIAIIGS ANINDRSLNG SRDSEICAII 

      1510       1520       1530       1540       1550       1560 
EDRDLVDSRV NGLPYKAAKF AHNLRCNLWE YHLGLISNPD PLLSDRIKDL VIDSTYHDIW 

      1570       1580       1590       1600       1610       1620 
RNMAQRNSAI YKEIFGTTIP ENCTKTSQYN RGSIKLTSEA LETLCGINGF LIEYNTSMLS 

      1630       1640 
ELETPTSIYS DIITSMKLFL

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References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Comparison of the hydrolysis of phosphatidylethanolamine and phosphatidyl(N-acyl)ethanolamine in Dictyostelium discoideum amoebae."
Ellingson J.S., Dischinger H.C.
Biochim. Biophys. Acta 796:155-162(1984) [PubMed: 6498208] [Abstract]
Cited for: FUNCTION.
[3]"Developmentally regulated changes in 1,2-diacylglycerol in Dictyostelium. Regulation by light and G proteins."
Cubitt A.B., Dharmawardhane S., Firtel R.A.
J. Biol. Chem. 268:17431-17439(1993) [PubMed: 8394342] [Abstract]
Cited for: FUNCTION.
[4]"A distant evolutionary relationship between GPI-specific phospholipase D and bacterial phosphatidylcholine-preferring phospholipase C."
Rigden D.J.
FEBS Lett. 569:229-234(2004) [PubMed: 15225639] [Abstract]
Cited for: NOMENCLATURE.
[5]"Phospholipase D activity is essential for actin localization and actin-based motility in Dictyostelium."
Zouwail S., Pettitt T.R., Dove S.K., Chibalina M.V., Powner D.J., Haynes L., Wakelam M.J.O., Insall R.H.
Biochem. J. 389:207-214(2005) [PubMed: 15769249] [Abstract]
Cited for: ENZYME REGULATION, FUNCTION.
[6]"Rho GTPase signaling in Dictyostelium discoideum: insights from the genome."
Vlahou G., Rivero F.
Eur. J. Cell Biol. 85:947-959(2006) [PubMed: 16762450] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AAFI02000023 Genomic DNA. Translation: EAL68147.1.
RefSeqXP_642027.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3393598.
GenomeReviewsGene locus pldC in contig CM000152_GR.
KEGGddi:DDB_0231508.

Organism-specific databases

dictyBaseDDB_G0277949. pldC.

Family and domain databases

InterProIPR015679. Phospholipase_D.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PANTHERPTHR18896. Phospholipase_D. 1 hit.
PfamPF00614. PLDc. 2 hits.
[Graphical view]
SMARTSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEPS50035. PLD. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePLDC_DICDI
AccessionPrimary (citable) accession number: Q54Z25
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 24, 2005
Last modified: September 22, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents