Q54YZ0 (UGPA2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase 2
EC=2.7.7.9

Alternative name(s):
UDP-glucose pyrophosphorylase 2
Short name=UDPGP 2
Short name=UGPase 2

Gene names

Name:	ugpB
ORF Names:	DDB_G0277879

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a central role as a glucosyl donor in cellular metabolic pathways. Ref.1
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Developmental stage	Expressed in both prestalk and prespore cells. Expression becomes strong after 12 hours of development and peaks at 16 hours. Ref.1
Disruption phenotype	Cells undergo aberrant differentiation and development ending with small, gnarled fruiting bodies. They also have decreased spore viability and decreased levels of glycogen. Ref.1
Sequence similarities	Belongs to the UDPGP type 1 family.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagic vacuole assembly Inferred from mutant phenotype. Source: dictyBase cellulose biosynthetic process Inferred from mutant phenotype. Source: dictyBase
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	UTP--glucose-1-phosphate uridylyltransferase 2		PRO_0000327942
Experimental info
	Sequence conflict	18	1	A → S in AAD34028. Ref.1
	Sequence conflict	320	1	I → V in AAA91057. Ref.3
	Sequence conflict	363 – 364	2	EI → RL in AAA91057. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q54YZ0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 3FC27BF59798F944	FASTA	502	56,268
	10 20 30 40 50 60 MMKPDLNSPL PQSPQLQAFG SRSSDLATED LFLKKLEAIS QTAPNETVKN EFLNKEIPSI 70 80 90 100 110 120 NKLFTRFLKN RKKVIDWDKI NPPPADMVLN YKDLPAITEQ RTSELASKLA VLKLNGGLGT 130 140 150 160 170 180 TMGCTGPKSV IEVRSEKTFL DLSVQQIKEM NERYNIKVPL VLMNSFNTHQ ETGKIIQKYK 190 200 210 220 230 240 YSDVKIHSFN QSRFPRILKD NLMPVPDKLF GSDSEWYPPG HGDVFFALQN SGLLETLINE 250 260 270 280 290 300 GKEYLFISNV DNLGAVVDFN ILEAMDKNKV EYIMEVTNKT RADVKGGTLI QYEGKAKLLE 310 320 330 340 350 360 IAQVPSSKVE EFKSIKKFKI FNTNNIWVNL KAMDRILKQN LLDDMDIIIN PKVADGKNII 370 380 390 400 410 420 QLEIAAGAAI EFFNNARGVN VPRSRFLPVK STSDLFIVQS NLYSLEKGVL VMNKNRPFTT 430 440 450 460 470 480 VPLVKLGDNF KKVSDYQARI KGIPDILELD QLTVSGDITF GPNMVLKGTV IIVANHGSRI 490 500 DIPEGSEFEN KVVSGNLHCG AL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second UDP-glucose pyrophosphorylase is required for differentiation and development in Dictyostelium discoideum." Bishop J.D., Moon B.C., Harrow F., Ratner D., Gomer R.H., Dottin R.P., Brazill D.T. J. Biol. Chem. 277:32430-32437(2002) [PubMed: 12060658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Null mutation of a UDPGP-glucose pyrophosphorylase gene by homologous recombination provides evidence for a second gene in Dictyostelium discoideum." Moon B.C., Haribabu B., Dottin R.P. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 319-370.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF150929 mRNA. Translation: AAD34028.1. AAFI02000023 Genomic DNA. Translation: EAL68112.1. L28007 Genomic DNA. Translation: AAA91057.1.
RefSeq	XP_642062.1. XM_636970.1.
3D structure databases
HSSP	HSSP built from PDB template 1Z90 based on UniProtKB Q9M9P3.
ProteinModelPortal	Q54YZ0.
SMR	Q54YZ0. Positions 55-496.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54YZ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0214911; DDB0214911; DDB_G0277879.
GeneID	8621274.
GenomeReviews	Gene locus ugpB in contig CM000152_GR.
KEGG	ddi:DDB_G0277879.
Organism-specific databases
dictyBase	DDB_G0277879. ugpB.
Phylogenomic databases
GeneTree	EPrGT00050000004511.
HOGENOM	HBG316482.
OMA	NDLTFLD.
PhylomeDB	Q54YZ0.
ProtClustDB	CLSZ2429721.
Family and domain databases
InterPro	IPR002618. UDPGP_trans. IPR016267. UDPGP_trans_subgr. [Graphical view]
KO	K00963.
PANTHER	PTHR11952:SF1. PTHR11952:SF1. 1 hit. PTHR11952. UDPGP_trans. 1 hit.
Pfam	PF01704. UDPGP. 1 hit. [Graphical view]
PIRSF	PIRSF000806. UDPGP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UGPA2_DICDI

Accession

Primary (citable) accession number: Q54YZ0
Secondary accession number(s): Q27566, Q9XZN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)