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Protein

Enoyl-[acyl-carrier-protein] reductase, mitochondrial

Gene

mecr

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro.By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69Proton donorBy similarity1
Binding sitei143NADPBy similarity1
Binding sitei343NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 172NADPBy similarity4
Nucleotide bindingi192 – 194NADPBy similarity3
Nucleotide bindingi261 – 264NADPBy similarity4
Nucleotide bindingi286 – 288NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNADP

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase, mitochondrial (EC:1.3.1.104)
Alternative name(s):
2-enoyl thioester reductase
Gene namesi
Name:mecr
ORF Names:DDB_G0278095
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278095. mecr.

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 14MitochondrionSequence analysisAdd BLAST14
ChainiPRO_000032831915 – 350Enoyl-[acyl-carrier-protein] reductase, mitochondrialAdd BLAST336

Proteomic databases

PaxDbiQ54YT4.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0235198.

Structurei

3D structure databases

ProteinModelPortaliQ54YT4.
SMRiQ54YT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0025. Eukaryota.
COG0604. LUCA.
InParanoidiQ54YT4.
KOiK07512.
OMAiHQLCRAW.
PhylomeDBiQ54YT4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SMARTiView protein in SMART
SM00829. PKS_ER. 1 hit.
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTRNIISL VRRYSTSRSV KIASHGSPST ALKIENENIT DKISNKDVLV
60 70 80 90 100
EMLHAPINPA DLNIIQGTYG TNVQVGGVAG MEGVGVVKKV GSGVTGLKEN
110 120 130 140 150
DLVVPSMKQH FGSWRSKGVW SEQQLFKVPS DIPTEYLSTI SINPTTAYLL
160 170 180 190 200
LNDFVKLQQG DVIIQNASNS MVGLSVIQLA KARGIKTINV IRDGSEFEDN
210 220 230 240 250
VQRLKQLGGD IVVSEEYVRT PAFRKLISDL PSPKLALNAV GGQSATELSR
260 270 280 290 300
ILADNGTLVT YGGMSREPVT IPTSQLIFRN IQIRGFWLNK WFEQHTDSEK
310 320 330 340 350
QSVYDAIFDL IRKKQFKLLI EKHKFSEFDQ ALLKSQQSGH GRKIVLDLQL
Length:350
Mass (Da):38,735
Last modified:May 24, 2005 - v1
Checksum:iD68461685025F443
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68220.1.
RefSeqiXP_642118.1. XM_637026.1.

Genome annotation databases

EnsemblProtistsiEAL68220; EAL68220; DDB_G0278095.
GeneIDi8621327.
KEGGiddi:DDB_G0278095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68220.1.
RefSeqiXP_642118.1. XM_637026.1.

3D structure databases

ProteinModelPortaliQ54YT4.
SMRiQ54YT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0235198.

Proteomic databases

PaxDbiQ54YT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL68220; EAL68220; DDB_G0278095.
GeneIDi8621327.
KEGGiddi:DDB_G0278095.

Organism-specific databases

dictyBaseiDDB_G0278095. mecr.

Phylogenomic databases

eggNOGiKOG0025. Eukaryota.
COG0604. LUCA.
InParanoidiQ54YT4.
KOiK07512.
OMAiHQLCRAW.
PhylomeDBiQ54YT4.

Miscellaneous databases

PROiPR:Q54YT4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SMARTiView protein in SMART
SM00829. PKS_ER. 1 hit.
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMECR_DICDI
AccessioniPrimary (citable) accession number: Q54YT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 12, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.