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Protein

Deoxyadenosine kinase

Gene

dak

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specific kinase that phosphorylates deoxyadenosine but not any other deoxyribonucleoside, as part of the deoxyribonucleotide salvage pathway.

Catalytic activityi

ATP + deoxyadenosine = ADP + dAMP.1 Publication

Kineticsi

Catalytic efficiency is 100-fold higher for deoxyadenosine than for thymidine.

  1. KM=4 µM for thymidine1 Publication
  2. KM=22.7 µM for deoxyadenosine1 Publication
  3. KM=146 µM for fludarabine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521SubstrateBy similarity
    Binding sitei64 – 641SubstrateBy similarity
    Binding sitei75 – 751SubstrateBy similarity
    Active sitei99 – 991Proton acceptorSequence Analysis
    Binding sitei100 – 1001SubstrateBy similarity
    Binding sitei105 – 1051SubstrateBy similarity
    Binding sitei165 – 1651SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 369ATPBy similarity

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • deoxyadenosine kinase activity Source: dictyBase

    GO - Biological processi

    • dAMP biosynthetic process Source: GOC
    • nucleobase-containing compound metabolic process Source: dictyBase
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKQ54YL2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyadenosine kinase (EC:2.7.1.76)
    Alternative name(s):
    DddDAK
    Short name:
    DAK
    Gene namesi
    Name:dak
    ORF Names:DDB_G0278191
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0278191. dak.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 245245Deoxyadenosine kinasePRO_0000327714Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB0232027.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54YL2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi84 – 885Poly-Gln

    Sequence similaritiesi

    Belongs to the DCK/DGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1428.
    InParanoidiQ54YL2.
    KOiK10353.
    OMAiKVDNNPY.
    PhylomeDBiQ54YL2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q54YL2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTTPILNSSV PGNIQKKSLE GTHIAISGLI GAGKTTLAVA LGKVLNLPTY
    60 70 80 90 100
    FEEVIDNLYL QDFYKDPKKY GFQLQIYLLN SRFQQQQQII WQARGGVQDR
    110 120 130 140 150
    TIYEDSVFAK MLNESGLLDD RDYNTYCKLF QNLSNFMRRP DLIIHLDVSP
    160 170 180 190 200
    EKSLERIKLR NRDCEKDVSL EYLQNLYNAY HEFLQDISRY IPVIRINWSE
    210 220 230 240
    FVDPEQLAQM IKAEYESMRF MNQINPPTFG NGPTTNKIIS TPKDL
    Length:245
    Mass (Da):28,416
    Last modified:May 24, 2005 - v1
    Checksum:iADA9B360DFE66B23
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY192983 mRNA. Translation: AAO64433.1.
    AAFI02000023 Genomic DNA. Translation: EAL68268.1.
    RefSeqiXP_642190.1. XM_637098.1.

    Genome annotation databases

    EnsemblProtistsiDDB0232027; DDB0232027; DDB_G0278191.
    GeneIDi8621397.
    KEGGiddi:DDB_G0278191.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY192983 mRNA. Translation: AAO64433.1.
    AAFI02000023 Genomic DNA. Translation: EAL68268.1.
    RefSeqiXP_642190.1. XM_637098.1.

    3D structure databases

    ProteinModelPortaliQ54YL2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi44689.DDB0232027.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblProtistsiDDB0232027; DDB0232027; DDB_G0278191.
    GeneIDi8621397.
    KEGGiddi:DDB_G0278191.

    Organism-specific databases

    dictyBaseiDDB_G0278191. dak.

    Phylogenomic databases

    eggNOGiCOG1428.
    InParanoidiQ54YL2.
    KOiK10353.
    OMAiKVDNNPY.
    PhylomeDBiQ54YL2.

    Enzyme and pathway databases

    SABIO-RKQ54YL2.

    Miscellaneous databases

    PROiQ54YL2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
      Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
      J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiDAK_DICDI
    AccessioniPrimary (citable) accession number: Q54YL2
    Secondary accession number(s): Q49UB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: June 24, 2015
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Can also efficiently phosphorylate medically important adenosine analogs, such as 9-beta-d-arabinofuranosyl-2-fluoroadenine (fludarabine).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.