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Q54YL2

- DAK_DICDI

UniProt

Q54YL2 - DAK_DICDI

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Protein
Deoxyadenosine kinase
Gene
dak, DDB_G0278191
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Specific kinase that phosphorylates deoxyadenosine but not any other deoxyribonucleoside, as part of the deoxyribonucleotide salvage pathway.

Catalytic activityi

ATP + deoxyadenosine = ADP + dAMP.1 Publication

Kineticsi

Catalytic efficiency is 100-fold higher for deoxyadenosine than for thymidine.

  1. KM=4 µM for thymidine1 Publication
  2. KM=22.7 µM for deoxyadenosine
  3. KM=146 µM for fludarabine

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521Substrate By similarity
Binding sitei64 – 641Substrate By similarity
Binding sitei75 – 751Substrate By similarity
Active sitei99 – 991Proton acceptor Reviewed prediction
Binding sitei100 – 1001Substrate By similarity
Binding sitei105 – 1051Substrate By similarity
Binding sitei165 – 1651Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 369ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. deoxyadenosine kinase activity Source: dictyBase
  3. phosphotransferase activity, alcohol group as acceptor Source: InterPro

GO - Biological processi

  1. dAMP biosynthetic process Source: GOC
  2. nucleobase-containing compound metabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.76. 1939.
SABIO-RKQ54YL2.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyadenosine kinase (EC:2.7.1.76)
Alternative name(s):
DddDAK
Short name:
DAK
Gene namesi
Name:dak
ORF Names:DDB_G0278191
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278191. dak.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Deoxyadenosine kinase
PRO_0000327714Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0232027.

Structurei

3D structure databases

ProteinModelPortaliQ54YL2.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi84 – 885Poly-Gln

Sequence similaritiesi

Belongs to the DCK/DGK family.

Phylogenomic databases

eggNOGiCOG1428.
OMAiKVDNNPY.
PhylomeDBiQ54YL2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54YL2-1 [UniParc]FASTAAdd to Basket

« Hide

MTTPILNSSV PGNIQKKSLE GTHIAISGLI GAGKTTLAVA LGKVLNLPTY    50
FEEVIDNLYL QDFYKDPKKY GFQLQIYLLN SRFQQQQQII WQARGGVQDR 100
TIYEDSVFAK MLNESGLLDD RDYNTYCKLF QNLSNFMRRP DLIIHLDVSP 150
EKSLERIKLR NRDCEKDVSL EYLQNLYNAY HEFLQDISRY IPVIRINWSE 200
FVDPEQLAQM IKAEYESMRF MNQINPPTFG NGPTTNKIIS TPKDL 245
Length:245
Mass (Da):28,416
Last modified:May 24, 2005 - v1
Checksum:iADA9B360DFE66B23
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY192983 mRNA. Translation: AAO64433.1.
AAFI02000023 Genomic DNA. Translation: EAL68268.1.
RefSeqiXP_642190.1. XM_637098.1.

Genome annotation databases

EnsemblProtistsiDDB0232027; DDB0232027; DDB_G0278191.
GeneIDi8621397.
KEGGiddi:DDB_G0278191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY192983 mRNA. Translation: AAO64433.1 .
AAFI02000023 Genomic DNA. Translation: EAL68268.1 .
RefSeqi XP_642190.1. XM_637098.1.

3D structure databases

ProteinModelPortali Q54YL2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0232027.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0232027 ; DDB0232027 ; DDB_G0278191 .
GeneIDi 8621397.
KEGGi ddi:DDB_G0278191.

Organism-specific databases

dictyBasei DDB_G0278191. dak.

Phylogenomic databases

eggNOGi COG1428.
OMAi KVDNNPY.
PhylomeDBi Q54YL2.

Enzyme and pathway databases

BRENDAi 2.7.1.76. 1939.
SABIO-RK Q54YL2.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10513. PTHR10513. 1 hit.
Pfami PF01712. dNK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000705. DNK. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
    Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
    J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiDAK_DICDI
AccessioniPrimary (citable) accession number: Q54YL2
Secondary accession number(s): Q49UB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Can also efficiently phosphorylate medically important adenosine analogs, such as 9-beta-d-arabinofuranosyl-2-fluoroadenine (fludarabine).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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