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Q54YL2 (DAK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyadenosine kinase

EC=2.7.1.76
Alternative name(s):
DddDAK
Short name=DAK
Gene names
Name:dak
ORF Names:DDB_G0278191
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specific kinase that phosphorylates deoxyadenosine but not any other deoxyribonucleoside, as part of the deoxyribonucleotide salvage pathway.

Catalytic activity

ATP + deoxyadenosine = ADP + dAMP. Ref.1

Miscellaneous

Can also efficiently phosphorylate medically important adenosine analogs, such as 9-beta-d-arabinofuranosyl-2-fluoroadenine (fludarabine).

Sequence similarities

Belongs to the DCK/DGK family.

Biophysicochemical properties

Kinetic parameters:

Catalytic efficiency is 100-fold higher for deoxyadenosine than for thymidine.

KM=4 µM for thymidine Ref.1

KM=22.7 µM for deoxyadenosine

KM=146 µM for fludarabine

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245Deoxyadenosine kinase
PRO_0000327714

Regions

Nucleotide binding28 – 369ATP By similarity
Compositional bias84 – 885Poly-Gln

Sites

Active site991Proton acceptor Potential
Binding site521Substrate By similarity
Binding site641Substrate By similarity
Binding site751Substrate By similarity
Binding site1001Substrate By similarity
Binding site1051Substrate By similarity
Binding site1651Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54YL2 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: ADA9B360DFE66B23

FASTA24528,416
        10         20         30         40         50         60 
MTTPILNSSV PGNIQKKSLE GTHIAISGLI GAGKTTLAVA LGKVLNLPTY FEEVIDNLYL 

        70         80         90        100        110        120 
QDFYKDPKKY GFQLQIYLLN SRFQQQQQII WQARGGVQDR TIYEDSVFAK MLNESGLLDD 

       130        140        150        160        170        180 
RDYNTYCKLF QNLSNFMRRP DLIIHLDVSP EKSLERIKLR NRDCEKDVSL EYLQNLYNAY 

       190        200        210        220        230        240 
HEFLQDISRY IPVIRINWSE FVDPEQLAQM IKAEYESMRF MNQINPPTFG NGPTTNKIIS 


TPKDL 

« Hide

References

« Hide 'large scale' references
[1]"Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY192983 mRNA. Translation: AAO64433.1.
AAFI02000023 Genomic DNA. Translation: EAL68268.1.
RefSeqXP_642190.1. XM_637098.1.

3D structure databases

ProteinModelPortalQ54YL2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0232027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0232027; DDB0232027; DDB_G0278191.
GeneID8621397.
KEGGddi:DDB_G0278191.

Organism-specific databases

dictyBaseDDB_G0278191. dak.

Phylogenomic databases

eggNOGCOG1428.
OMAFEDANIF.
ProtClustDBCLSZ2430595.

Enzyme and pathway databases

BRENDA2.7.1.76. 1939.
SABIO-RKQ54YL2.

Family and domain databases

InterProIPR002624. Deoxynucleoside_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10513. PTHR10513. 1 hit.
PfamPF01712. dNK. 1 hit.
[Graphical view]
PIRSFPIRSF000705. DNK. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDAK_DICDI
AccessionPrimary (citable) accession number: Q54YL2
Secondary accession number(s): Q49UB1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: October 16, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase