Q54YL2 (DAK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyadenosine kinase
EC=2.7.1.76

Alternative name(s):
DddDAK
Short name=DAK

Gene names

Name:	dak
ORF Names:	DDB_G0278191

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specific kinase that phosphorylates deoxyadenosine but not any other deoxyribonucleoside, as part of the deoxyribonucleotide salvage pathway.
Catalytic activity	ATP + deoxyadenosine = ADP + dAMP. Ref.1
Miscellaneous	Can also efficiently phosphorylate medically important adenosine analogs, such as 9-beta-d-arabinofuranosyl-2-fluoroadenine (fludarabine).
Sequence similarities	Belongs to the DCK/DGK family.
Biophysicochemical properties	Kinetic parameters: Catalytic efficiency is 100-fold higher for deoxyadenosine than for thymidine. KM=4 µM for thymidine Ref.1 KM=22.7 µM for deoxyadenosine KM=146 µM for fludarabine

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW deoxyadenosine kinase activity Inferred from direct assay Ref.1. Source: dictyBase phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Deoxyadenosine kinase		PRO_0000327714
Regions
	Nucleotide binding	28 – 36	9	ATP By similarity
	Compositional bias	84 – 88	5	Poly-Gln
Sites
	Active site	99	1	Proton acceptor Potential
	Binding site	52	1	Substrate By similarity
	Binding site	64	1	Substrate By similarity
	Binding site	75	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54YL2 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: ADA9B360DFE66B23	FASTA	245	28,416
	10 20 30 40 50 60 MTTPILNSSV PGNIQKKSLE GTHIAISGLI GAGKTTLAVA LGKVLNLPTY FEEVIDNLYL 70 80 90 100 110 120 QDFYKDPKKY GFQLQIYLLN SRFQQQQQII WQARGGVQDR TIYEDSVFAK MLNESGLLDD 130 140 150 160 170 180 RDYNTYCKLF QNLSNFMRRP DLIIHLDVSP EKSLERIKLR NRDCEKDVSL EYLQNLYNAY 190 200 210 220 230 240 HEFLQDISRY IPVIRINWSE FVDPEQLAQM IKAEYESMRF MNQINPPTFG NGPTTNKIIS TPKDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases." Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J. J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY192983 mRNA. Translation: AAO64433.1. AAFI02000023 Genomic DNA. Translation: EAL68268.1.
RefSeq	XP_642190.1. XM_637098.1.
3D structure databases
HSSP	HSSP built from PDB template 1OT3 based on UniProtKB Q9XZT6.
ProteinModelPortal	Q54YL2.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0232027.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0232027; DDB0232027; DDB_G0278191.
GeneID	8621397.
KEGG	ddi:DDB_G0278191.
Organism-specific databases
dictyBase	DDB_G0278191. dak.
Phylogenomic databases
eggNOG	COG1428.
OMA	YETGIDA.
ProtClustDB	CLSZ2430595.
Enzyme and pathway databases
BRENDA	2.7.1.76. 1939.
SABIO-RK	Q54YL2.
Family and domain databases
InterPro	IPR002624. Deoxynucleoside_kinase. [Graphical view]
PANTHER	PTHR10513. PTHR10513. 1 hit.
Pfam	PF01712. dNK. 1 hit. [Graphical view]
PIRSF	PIRSF000705. DNK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAK_DICDI

Accession

Primary (citable) accession number: Q54YL2
Secondary accession number(s): Q49UB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)