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Q54YF7 (MANB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-mannosidase B

EC=3.2.1.24
Gene names
Name:manB
ORF Names:DDB_G0278259
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmannose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 10351015Alpha-mannosidase B
PRO_0000327841

Regions

Compositional bias618 – 6225Poly-Thr
Compositional bias731 – 7366Poly-Asn
Compositional bias976 – 98611Poly-Asn

Sites

Active site1611Nucleophile By similarity
Metal binding471Zinc By similarity
Metal binding491Zinc By similarity
Metal binding1611Zinc By similarity
Metal binding4461Zinc By similarity

Amino acid modifications

Glycosylation191N-linked (GlcNAc...) Potential
Glycosylation301N-linked (GlcNAc...) Potential
Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation2451N-linked (GlcNAc...) Potential
Glycosylation2501N-linked (GlcNAc...) Potential
Glycosylation2701N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation3271N-linked (GlcNAc...) Potential
Glycosylation4381N-linked (GlcNAc...) Potential
Glycosylation4871N-linked (GlcNAc...) Potential
Glycosylation4971N-linked (GlcNAc...) Potential
Glycosylation5031N-linked (GlcNAc...) Potential
Glycosylation7101N-linked (GlcNAc...) Potential
Glycosylation7191N-linked (GlcNAc...) Potential
Glycosylation7351N-linked (GlcNAc...) Potential
Glycosylation7921N-linked (GlcNAc...) Potential
Glycosylation8521N-linked (GlcNAc...) Potential
Glycosylation8631N-linked (GlcNAc...) Potential
Glycosylation8801N-linked (GlcNAc...) Potential
Glycosylation9621N-linked (GlcNAc...) Potential
Glycosylation9931N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q54YF7 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: A48F295BD41AA936

FASTA1,035119,087
        10         20         30         40         50         60 
MGKVLILFLF VLLLITFINC TNNKILNNEN SSNEDNVINV FLIPHSHCDL GWVQTLEEYY 

        70         80         90        100        110        120 
SENVTVILDN VINTLIKDSS KKFNWAEIYY FETWWNEQSS FLQAQVRNLV NNGQLYFVGG 

       130        140        150        160        170        180 
GWAQNDEGAT HYQAVINQMT LGHQFLLSEF GVVPEIGWQI DPFGPSTLTA TLFSLMGFKY 

       190        200        210        220        230        240 
HVINRIDERI KYIYNDTPDI VGSGWMTTDR SFEFQWYPSH NDQELSIFTH VLDHHYNSPY 

       250        260        270        280        290        300 
LIYPNASNPN QSLTTGFDFE SDPTQNPPIN QSNIYERAAV FVEIMQQRSQ LYRHNNLLIP 

       310        320        330        340        350        360 
FGNDFRFQNA SLEFDNMDKL ITYINSNSSW GVTIQYATLN EYFEKVESIE PPVEYADIVG 

       370        380        390        400        410        420 
QDLFVYTMCL ASDYQAFNTC ANWWSGYYTS YPLLKQTARD SDSLLRVGEM LYSLSCFYSN 

       430        440        450        460        470        480 
GFDFDFNIGF YALSMHRNVS GILTHHDAIT GTAKEYVRVN YFQMLNEAQS LTLDYNIPDF 

       490        500        510        520        530        540 
VGFLLSNKSL NIDYQSNGSS ILNSTNPGDI IAISFTNSIA WDRIETVSIE IPFVNMAVYD 

       550        560        570        580        590        600 
YQLNPIQSQI VQRFDKSNNW YLYFQVATPA LGISTYFIVI LSTDGEILND NSKNLPTPIQ 

       610        620        630        640        650        660 
SILSISNELL FNDNDDETTT TTIGNSNFNL NFKFDRDNNN LLTLNSYDDL FNNKIGIPIS 

       670        680        690        700        710        720 
QHLIEYTSLS DDAYKFRVQG LPIPLTPINP QFYLTIGPVV QIVTIIYNNN CSQSYLIYND 

       730        740        750        760        770        780 
TTSFNPFETD NDNNNNSRLI KNDQYFEIDN IVASGWDKEI SMKFTTNINN QNIFYTNNGL 

       790        800        810        820        830        840 
EIMKRQWEIH WNDTFIWSEI TSNFYPMINT GYIVDQQVSD YQQQLTILSK QTFGASSQTN 

       850        860        870        880        890        900 
GEFEVLLIRR SNYTQWSVHE PMNDTSNPSL RVLCIFGDPN FSNEIRTPHS ILFENPLQPV 

       910        920        930        940        950        960 
YSLIPNSIPI EKYIDTYNTL FKPLQTSLPY NLHLLTFTKQ WIDSPSIIMR LINIYEIGQS 

       970        980        990       1000       1010       1020 
INFSKSINFN IGSGDNNNNN NNNNNNGFLT HYNISQIIET TLSANSELSN PTNNLVITLE 

      1030 
PLEIKTFLLT LSPKQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000023 Genomic DNA. Translation: EAL68302.1.
RefSeqXP_642245.1. XM_637153.1.

3D structure databases

ProteinModelPortalQ54YF7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231612.

Proteomic databases

PRIDEQ54YF7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231612; DDB0231612; DDB_G0278259.
GeneID8621454.
KEGGddi:DDB_G0278259.

Organism-specific databases

dictyBaseDDB_G0278259. manB.

Phylogenomic databases

eggNOGCOG0383.
OMADESVSHW.
PhylomeDBQ54YF7.

Family and domain databases

Gene3D1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Other

PROQ54YF7.

Entry information

Entry nameMANB_DICDI
AccessionPrimary (citable) accession number: Q54YF7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase