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Q54YF7

- MANB_DICDI

UniProt

Q54YF7 - MANB_DICDI

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Protein
Alpha-mannosidase B
Gene
manB, DDB_G0278259
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Zinc By similarity
Metal bindingi49 – 491Zinc By similarity
Active sitei161 – 1611Nucleophile By similarity
Metal bindingi161 – 1611Zinc By similarity
Metal bindingi446 – 4461Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase B (EC:3.2.1.24)
Gene namesi
Name:manB
ORF Names:DDB_G0278259
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278259. manB.

Subcellular locationi

Secreted Reviewed prediction

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 10351015Alpha-mannosidase B
PRO_0000327841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi19 – 191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi30 – 301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi63 – 631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi245 – 2451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi250 – 2501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi270 – 2701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi309 – 3091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi327 – 3271N-linked (GlcNAc...) Reviewed prediction
Glycosylationi438 – 4381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi487 – 4871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi497 – 4971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi503 – 5031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi710 – 7101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi719 – 7191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi735 – 7351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi792 – 7921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi852 – 8521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi863 – 8631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi880 – 8801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi962 – 9621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi993 – 9931N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ54YF7.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231612.

Structurei

3D structure databases

ProteinModelPortaliQ54YF7.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi618 – 6225Poly-Thr
Compositional biasi731 – 7366Poly-Asn
Compositional biasi976 – 98611Poly-Asn
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0383.
OMAiDESVSHW.
PhylomeDBiQ54YF7.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YF7-1 [UniParc]FASTAAdd to Basket

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MGKVLILFLF VLLLITFINC TNNKILNNEN SSNEDNVINV FLIPHSHCDL     50
GWVQTLEEYY SENVTVILDN VINTLIKDSS KKFNWAEIYY FETWWNEQSS 100
FLQAQVRNLV NNGQLYFVGG GWAQNDEGAT HYQAVINQMT LGHQFLLSEF 150
GVVPEIGWQI DPFGPSTLTA TLFSLMGFKY HVINRIDERI KYIYNDTPDI 200
VGSGWMTTDR SFEFQWYPSH NDQELSIFTH VLDHHYNSPY LIYPNASNPN 250
QSLTTGFDFE SDPTQNPPIN QSNIYERAAV FVEIMQQRSQ LYRHNNLLIP 300
FGNDFRFQNA SLEFDNMDKL ITYINSNSSW GVTIQYATLN EYFEKVESIE 350
PPVEYADIVG QDLFVYTMCL ASDYQAFNTC ANWWSGYYTS YPLLKQTARD 400
SDSLLRVGEM LYSLSCFYSN GFDFDFNIGF YALSMHRNVS GILTHHDAIT 450
GTAKEYVRVN YFQMLNEAQS LTLDYNIPDF VGFLLSNKSL NIDYQSNGSS 500
ILNSTNPGDI IAISFTNSIA WDRIETVSIE IPFVNMAVYD YQLNPIQSQI 550
VQRFDKSNNW YLYFQVATPA LGISTYFIVI LSTDGEILND NSKNLPTPIQ 600
SILSISNELL FNDNDDETTT TTIGNSNFNL NFKFDRDNNN LLTLNSYDDL 650
FNNKIGIPIS QHLIEYTSLS DDAYKFRVQG LPIPLTPINP QFYLTIGPVV 700
QIVTIIYNNN CSQSYLIYND TTSFNPFETD NDNNNNSRLI KNDQYFEIDN 750
IVASGWDKEI SMKFTTNINN QNIFYTNNGL EIMKRQWEIH WNDTFIWSEI 800
TSNFYPMINT GYIVDQQVSD YQQQLTILSK QTFGASSQTN GEFEVLLIRR 850
SNYTQWSVHE PMNDTSNPSL RVLCIFGDPN FSNEIRTPHS ILFENPLQPV 900
YSLIPNSIPI EKYIDTYNTL FKPLQTSLPY NLHLLTFTKQ WIDSPSIIMR 950
LINIYEIGQS INFSKSINFN IGSGDNNNNN NNNNNNGFLT HYNISQIIET 1000
TLSANSELSN PTNNLVITLE PLEIKTFLLT LSPKQ 1035
Length:1,035
Mass (Da):119,087
Last modified:May 24, 2005 - v1
Checksum:iA48F295BD41AA936
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68302.1.
RefSeqiXP_642245.1. XM_637153.1.

Genome annotation databases

EnsemblProtistsiDDB0231612; DDB0231612; DDB_G0278259.
GeneIDi8621454.
KEGGiddi:DDB_G0278259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68302.1 .
RefSeqi XP_642245.1. XM_637153.1.

3D structure databases

ProteinModelPortali Q54YF7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231612.

Proteomic databases

PRIDEi Q54YF7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231612 ; DDB0231612 ; DDB_G0278259 .
GeneIDi 8621454.
KEGGi ddi:DDB_G0278259.

Organism-specific databases

dictyBasei DDB_G0278259. manB.

Phylogenomic databases

eggNOGi COG0383.
OMAi DESVSHW.
PhylomeDBi Q54YF7.

Enzyme and pathway databases

Reactomei REACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

PROi Q54YF7.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANB_DICDI
AccessioniPrimary (citable) accession number: Q54YF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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