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Protein

Alpha-mannosidase B

Gene

manB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47ZincBy similarity1
Metal bindingi49ZincBy similarity1
Active sitei161NucleophileBy similarity1
Metal bindingi161ZincBy similarity1
Metal bindingi446ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-975578 Reactions specific to the complex N-glycan synthesis pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase B (EC:3.2.1.24)
Gene namesi
Name:manB
ORF Names:DDB_G0278259
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278259 manB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000032784121 – 1035Alpha-mannosidase BAdd BLAST1015

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi19N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi30N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi63N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi245N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi250N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi309N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi327N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi438N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi487N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi497N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi503N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi710N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi719N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi735N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi792N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi852N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi863N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi880N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi962N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi993N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ54YF7

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231612

Structurei

3D structure databases

ProteinModelPortaliQ54YF7
SMRiQ54YF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi618 – 622Poly-Thr5
Compositional biasi731 – 736Poly-Asn6
Compositional biasi976 – 986Poly-AsnAdd BLAST11

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1959 Eukaryota
ENOG410XQMZ LUCA
InParanoidiQ54YF7
OMAiEFMWRQT
PhylomeDBiQ54YF7

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
2.60.40.1180, 1 hit
3.20.110.10, 1 hit
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR011682 Glyco_hydro_38_C
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
IPR013780 Glyco_hydro_b
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 1 hit
PF07748 Glyco_hydro_38C, 1 hit
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLILFLF VLLLITFINC TNNKILNNEN SSNEDNVINV FLIPHSHCDL
60 70 80 90 100
GWVQTLEEYY SENVTVILDN VINTLIKDSS KKFNWAEIYY FETWWNEQSS
110 120 130 140 150
FLQAQVRNLV NNGQLYFVGG GWAQNDEGAT HYQAVINQMT LGHQFLLSEF
160 170 180 190 200
GVVPEIGWQI DPFGPSTLTA TLFSLMGFKY HVINRIDERI KYIYNDTPDI
210 220 230 240 250
VGSGWMTTDR SFEFQWYPSH NDQELSIFTH VLDHHYNSPY LIYPNASNPN
260 270 280 290 300
QSLTTGFDFE SDPTQNPPIN QSNIYERAAV FVEIMQQRSQ LYRHNNLLIP
310 320 330 340 350
FGNDFRFQNA SLEFDNMDKL ITYINSNSSW GVTIQYATLN EYFEKVESIE
360 370 380 390 400
PPVEYADIVG QDLFVYTMCL ASDYQAFNTC ANWWSGYYTS YPLLKQTARD
410 420 430 440 450
SDSLLRVGEM LYSLSCFYSN GFDFDFNIGF YALSMHRNVS GILTHHDAIT
460 470 480 490 500
GTAKEYVRVN YFQMLNEAQS LTLDYNIPDF VGFLLSNKSL NIDYQSNGSS
510 520 530 540 550
ILNSTNPGDI IAISFTNSIA WDRIETVSIE IPFVNMAVYD YQLNPIQSQI
560 570 580 590 600
VQRFDKSNNW YLYFQVATPA LGISTYFIVI LSTDGEILND NSKNLPTPIQ
610 620 630 640 650
SILSISNELL FNDNDDETTT TTIGNSNFNL NFKFDRDNNN LLTLNSYDDL
660 670 680 690 700
FNNKIGIPIS QHLIEYTSLS DDAYKFRVQG LPIPLTPINP QFYLTIGPVV
710 720 730 740 750
QIVTIIYNNN CSQSYLIYND TTSFNPFETD NDNNNNSRLI KNDQYFEIDN
760 770 780 790 800
IVASGWDKEI SMKFTTNINN QNIFYTNNGL EIMKRQWEIH WNDTFIWSEI
810 820 830 840 850
TSNFYPMINT GYIVDQQVSD YQQQLTILSK QTFGASSQTN GEFEVLLIRR
860 870 880 890 900
SNYTQWSVHE PMNDTSNPSL RVLCIFGDPN FSNEIRTPHS ILFENPLQPV
910 920 930 940 950
YSLIPNSIPI EKYIDTYNTL FKPLQTSLPY NLHLLTFTKQ WIDSPSIIMR
960 970 980 990 1000
LINIYEIGQS INFSKSINFN IGSGDNNNNN NNNNNNGFLT HYNISQIIET
1010 1020 1030
TLSANSELSN PTNNLVITLE PLEIKTFLLT LSPKQ
Length:1,035
Mass (Da):119,087
Last modified:May 24, 2005 - v1
Checksum:iA48F295BD41AA936
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA Translation: EAL68302.1
RefSeqiXP_642245.1, XM_637153.1

Genome annotation databases

EnsemblProtistsiEAL68302; EAL68302; DDB_G0278259
GeneIDi8621454
KEGGiddi:DDB_G0278259

Similar proteinsi

Entry informationi

Entry nameiMANB_DICDI
AccessioniPrimary (citable) accession number: Q54YF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
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