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Protein

Alpha-mannosidase B

Gene

manB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47ZincBy similarity1
Metal bindingi49ZincBy similarity1
Active sitei161NucleophileBy similarity1
Metal bindingi161ZincBy similarity1
Metal bindingi446ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase B (EC:3.2.1.24)
Gene namesi
Name:manB
ORF Names:DDB_G0278259
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278259. manB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000032784121 – 1035Alpha-mannosidase BAdd BLAST1015

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi19N-linked (GlcNAc...)Sequence analysis1
Glycosylationi30N-linked (GlcNAc...)Sequence analysis1
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi245N-linked (GlcNAc...)Sequence analysis1
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Glycosylationi270N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi438N-linked (GlcNAc...)Sequence analysis1
Glycosylationi487N-linked (GlcNAc...)Sequence analysis1
Glycosylationi497N-linked (GlcNAc...)Sequence analysis1
Glycosylationi503N-linked (GlcNAc...)Sequence analysis1
Glycosylationi710N-linked (GlcNAc...)Sequence analysis1
Glycosylationi719N-linked (GlcNAc...)Sequence analysis1
Glycosylationi735N-linked (GlcNAc...)Sequence analysis1
Glycosylationi792N-linked (GlcNAc...)Sequence analysis1
Glycosylationi852N-linked (GlcNAc...)Sequence analysis1
Glycosylationi863N-linked (GlcNAc...)Sequence analysis1
Glycosylationi880N-linked (GlcNAc...)Sequence analysis1
Glycosylationi962N-linked (GlcNAc...)Sequence analysis1
Glycosylationi993N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ54YF7.

Structurei

3D structure databases

ProteinModelPortaliQ54YF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi618 – 622Poly-Thr5
Compositional biasi731 – 736Poly-Asn6
Compositional biasi976 – 986Poly-AsnAdd BLAST11

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ54YF7.
OMAiFNTCANW.
PhylomeDBiQ54YF7.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLILFLF VLLLITFINC TNNKILNNEN SSNEDNVINV FLIPHSHCDL
60 70 80 90 100
GWVQTLEEYY SENVTVILDN VINTLIKDSS KKFNWAEIYY FETWWNEQSS
110 120 130 140 150
FLQAQVRNLV NNGQLYFVGG GWAQNDEGAT HYQAVINQMT LGHQFLLSEF
160 170 180 190 200
GVVPEIGWQI DPFGPSTLTA TLFSLMGFKY HVINRIDERI KYIYNDTPDI
210 220 230 240 250
VGSGWMTTDR SFEFQWYPSH NDQELSIFTH VLDHHYNSPY LIYPNASNPN
260 270 280 290 300
QSLTTGFDFE SDPTQNPPIN QSNIYERAAV FVEIMQQRSQ LYRHNNLLIP
310 320 330 340 350
FGNDFRFQNA SLEFDNMDKL ITYINSNSSW GVTIQYATLN EYFEKVESIE
360 370 380 390 400
PPVEYADIVG QDLFVYTMCL ASDYQAFNTC ANWWSGYYTS YPLLKQTARD
410 420 430 440 450
SDSLLRVGEM LYSLSCFYSN GFDFDFNIGF YALSMHRNVS GILTHHDAIT
460 470 480 490 500
GTAKEYVRVN YFQMLNEAQS LTLDYNIPDF VGFLLSNKSL NIDYQSNGSS
510 520 530 540 550
ILNSTNPGDI IAISFTNSIA WDRIETVSIE IPFVNMAVYD YQLNPIQSQI
560 570 580 590 600
VQRFDKSNNW YLYFQVATPA LGISTYFIVI LSTDGEILND NSKNLPTPIQ
610 620 630 640 650
SILSISNELL FNDNDDETTT TTIGNSNFNL NFKFDRDNNN LLTLNSYDDL
660 670 680 690 700
FNNKIGIPIS QHLIEYTSLS DDAYKFRVQG LPIPLTPINP QFYLTIGPVV
710 720 730 740 750
QIVTIIYNNN CSQSYLIYND TTSFNPFETD NDNNNNSRLI KNDQYFEIDN
760 770 780 790 800
IVASGWDKEI SMKFTTNINN QNIFYTNNGL EIMKRQWEIH WNDTFIWSEI
810 820 830 840 850
TSNFYPMINT GYIVDQQVSD YQQQLTILSK QTFGASSQTN GEFEVLLIRR
860 870 880 890 900
SNYTQWSVHE PMNDTSNPSL RVLCIFGDPN FSNEIRTPHS ILFENPLQPV
910 920 930 940 950
YSLIPNSIPI EKYIDTYNTL FKPLQTSLPY NLHLLTFTKQ WIDSPSIIMR
960 970 980 990 1000
LINIYEIGQS INFSKSINFN IGSGDNNNNN NNNNNNGFLT HYNISQIIET
1010 1020 1030
TLSANSELSN PTNNLVITLE PLEIKTFLLT LSPKQ
Length:1,035
Mass (Da):119,087
Last modified:May 24, 2005 - v1
Checksum:iA48F295BD41AA936
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68302.1.
RefSeqiXP_642245.1. XM_637153.1.

Genome annotation databases

EnsemblProtistsiEAL68302; EAL68302; DDB_G0278259.
GeneIDi8621454.
KEGGiddi:DDB_G0278259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68302.1.
RefSeqiXP_642245.1. XM_637153.1.

3D structure databases

ProteinModelPortaliQ54YF7.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ54YF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL68302; EAL68302; DDB_G0278259.
GeneIDi8621454.
KEGGiddi:DDB_G0278259.

Organism-specific databases

dictyBaseiDDB_G0278259. manB.

Phylogenomic databases

eggNOGiKOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ54YF7.
OMAiFNTCANW.
PhylomeDBiQ54YF7.

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

PROiQ54YF7.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMANB_DICDI
AccessioniPrimary (citable) accession number: Q54YF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.