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Protein

Alpha-mannosidase B

Gene

manB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471ZincBy similarity
Metal bindingi49 – 491ZincBy similarity
Active sitei161 – 1611NucleophileBy similarity
Metal bindingi161 – 1611ZincBy similarity
Metal bindingi446 – 4461ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase B (EC:3.2.1.24)
Gene namesi
Name:manB
ORF Names:DDB_G0278259
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278259. manB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 10351015Alpha-mannosidase BPRO_0000327841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi19 – 191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi438 – 4381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi503 – 5031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi710 – 7101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi719 – 7191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi792 – 7921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi852 – 8521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi863 – 8631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi880 – 8801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi962 – 9621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi993 – 9931N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ54YF7.

Structurei

3D structure databases

ProteinModelPortaliQ54YF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi618 – 6225Poly-Thr
Compositional biasi731 – 7366Poly-Asn
Compositional biasi976 – 98611Poly-AsnAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0383.
InParanoidiQ54YF7.
OMAiMEDWTIS.
PhylomeDBiQ54YF7.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLILFLF VLLLITFINC TNNKILNNEN SSNEDNVINV FLIPHSHCDL
60 70 80 90 100
GWVQTLEEYY SENVTVILDN VINTLIKDSS KKFNWAEIYY FETWWNEQSS
110 120 130 140 150
FLQAQVRNLV NNGQLYFVGG GWAQNDEGAT HYQAVINQMT LGHQFLLSEF
160 170 180 190 200
GVVPEIGWQI DPFGPSTLTA TLFSLMGFKY HVINRIDERI KYIYNDTPDI
210 220 230 240 250
VGSGWMTTDR SFEFQWYPSH NDQELSIFTH VLDHHYNSPY LIYPNASNPN
260 270 280 290 300
QSLTTGFDFE SDPTQNPPIN QSNIYERAAV FVEIMQQRSQ LYRHNNLLIP
310 320 330 340 350
FGNDFRFQNA SLEFDNMDKL ITYINSNSSW GVTIQYATLN EYFEKVESIE
360 370 380 390 400
PPVEYADIVG QDLFVYTMCL ASDYQAFNTC ANWWSGYYTS YPLLKQTARD
410 420 430 440 450
SDSLLRVGEM LYSLSCFYSN GFDFDFNIGF YALSMHRNVS GILTHHDAIT
460 470 480 490 500
GTAKEYVRVN YFQMLNEAQS LTLDYNIPDF VGFLLSNKSL NIDYQSNGSS
510 520 530 540 550
ILNSTNPGDI IAISFTNSIA WDRIETVSIE IPFVNMAVYD YQLNPIQSQI
560 570 580 590 600
VQRFDKSNNW YLYFQVATPA LGISTYFIVI LSTDGEILND NSKNLPTPIQ
610 620 630 640 650
SILSISNELL FNDNDDETTT TTIGNSNFNL NFKFDRDNNN LLTLNSYDDL
660 670 680 690 700
FNNKIGIPIS QHLIEYTSLS DDAYKFRVQG LPIPLTPINP QFYLTIGPVV
710 720 730 740 750
QIVTIIYNNN CSQSYLIYND TTSFNPFETD NDNNNNSRLI KNDQYFEIDN
760 770 780 790 800
IVASGWDKEI SMKFTTNINN QNIFYTNNGL EIMKRQWEIH WNDTFIWSEI
810 820 830 840 850
TSNFYPMINT GYIVDQQVSD YQQQLTILSK QTFGASSQTN GEFEVLLIRR
860 870 880 890 900
SNYTQWSVHE PMNDTSNPSL RVLCIFGDPN FSNEIRTPHS ILFENPLQPV
910 920 930 940 950
YSLIPNSIPI EKYIDTYNTL FKPLQTSLPY NLHLLTFTKQ WIDSPSIIMR
960 970 980 990 1000
LINIYEIGQS INFSKSINFN IGSGDNNNNN NNNNNNGFLT HYNISQIIET
1010 1020 1030
TLSANSELSN PTNNLVITLE PLEIKTFLLT LSPKQ
Length:1,035
Mass (Da):119,087
Last modified:May 24, 2005 - v1
Checksum:iA48F295BD41AA936
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68302.1.
RefSeqiXP_642245.1. XM_637153.1.

Genome annotation databases

EnsemblProtistsiDDB0231612; DDB0231612; DDB_G0278259.
GeneIDi8621454.
KEGGiddi:DDB_G0278259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68302.1.
RefSeqiXP_642245.1. XM_637153.1.

3D structure databases

ProteinModelPortaliQ54YF7.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ54YF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231612; DDB0231612; DDB_G0278259.
GeneIDi8621454.
KEGGiddi:DDB_G0278259.

Organism-specific databases

dictyBaseiDDB_G0278259. manB.

Phylogenomic databases

eggNOGiCOG0383.
InParanoidiQ54YF7.
OMAiMEDWTIS.
PhylomeDBiQ54YF7.

Miscellaneous databases

PROiQ54YF7.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMANB_DICDI
AccessioniPrimary (citable) accession number: Q54YF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.