Reviewed, UniProtKB/Swiss-Prot Q54YF2 (AMPKA_DICDI)

Names and origin

Protein names

Recommended name:
    5'-AMP-activated serine/threonine-protein kinase catalytic subunit alpha
      Short name=AMPKA
    EC=2.7.11.1
Alternative name(s):
    Protein kinase, AMP-activated, alpha subunit
    SNF1/AMP-activated kinase catalytic subunit
    Sucrose non-fermenting protein snfA

Gene names

Name:	snfA
Synonyms:	ampka, prkaa
ORF Names:	DDB_G0277905

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activated enzyme phosphorylates target proteins and initiates downstream signaling pathways that shift metabolism from anabolic to catabolic pathways. Acts as a highly sensitive cellular energy sensor. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits Potential.
Developmental stage	Expressed throughout development. Levels may increase during the first 5 hours of starvation-induced differentiation. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain.
Sequence caution	The sequence EAL68125.1 differs from that shown. Reason: Frameshift at position 715.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Ref.3 Inferred from mutant phenotype. Source: dictyBase cell proliferation Ref.3 Inferred from mutant phenotype. Source: dictyBase mitochondrion organization Ref.3 Inferred from mutant phenotype. Source: dictyBase positive phototaxis Ref.3 Inferred from mutant phenotype. Source: dictyBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	5'-AMP-activated serine/threonine-protein kinase catalytic subunit alpha		PRO_0000358880
Regions
	Domain	31 – 284	254	Protein kinase
	Domain	679 – 727	49	KA1
	Nucleotide binding	37 – 45	9	ATP By similarity
	Compositional bias	383 – 386	4	Poly-Thr
	Compositional bias	398 – 409	12	Poly-Asn
	Compositional bias	412 – 416	5	Poly-Asn
	Compositional bias	419 – 422	4	Poly-Asn
	Compositional bias	426 – 450	25	Poly-Asn
	Compositional bias	453 – 479	27	Poly-Asn
	Compositional bias	511 – 550	40	Poly-Asn
	Compositional bias	566 – 577	12	Poly-Asn
Sites
	Active site	154	1	Proton acceptor By similarity
	Binding site	60	1	ATP By similarity
Amino acid modifications
	Modified residue	188	1	Phosphothreonine Potential
Experimental info
	Sequence conflict	3	1	S → P in AAD30963. Ref.1
	Sequence conflict	12	1	F → L in AAD30963. Ref.1
	Sequence conflict	49	1	E → K in AAD30963. Ref.1
	Sequence conflict	64	1	R → K in AAD30963. Ref.1
	Sequence conflict	91 – 93	3	IIK → FIN in AAD30963. Ref.1
	Sequence conflict	105	1	F → P in AAD30963. Ref.1
	Sequence conflict	112	1	T → P in AAD30963. Ref.1
	Sequence conflict	117 – 118	2	FE → LD in AAD30963. Ref.1
	Sequence conflict	122	1	K → R in AAD30963. Ref.1
	Sequence conflict	125	1	K → N in AAD30963. Ref.1
	Sequence conflict	134	1	L → P in AAD30963. Ref.1
	Sequence conflict	143	1	D → G in AAD30963. Ref.1
	Sequence conflict	328 – 329	2	EE → GG in AAD30963. Ref.1
	Sequence conflict	355 – 359	5	ENEIN → DDEVY in AAD30963. Ref.1
	Sequence conflict	376 – 378	3	QKS → PQR in AAD30963. Ref.1
	Sequence conflict	391	1	S → T in AAD30963. Ref.1
	Sequence conflict	453	1	Missing in AAD30963. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54YF2-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 481E0242FD2AB181	FASTA	727	81,639
	10 20 30 40 50 60 MSSYQQNPIG SFGGLYGSGG IEKSSQIIGN YRLDKTLGIG SFGKVKLAEH IRTGVKVAIK 70 80 90 100 110 120 ILNRTKIKNL KMDEKIRREI QNMKLFRHPH IIKLYEVIET TTDIFMVMEY VTGGELFEYI 130 140 150 160 170 180 VKNGKLLEDE SRRLFQQMIS GVDYCHHHMV VHRDLKPENL LLDPINKCIK IADFGLSNMM 190 200 210 220 230 240 QDGDFLKTSC GSPNYAAPEV ISGKLYAGPE VDVWSCGVIL YAFLCAKLPF DDESIPMLFK 250 260 270 280 290 300 KIREGVFSIP DFVSPSCADL IKKMLVVDPV KRITIHEIRN HPWFQVKLPK YLSSPHTFLS 310 320 330 340 350 360 KSIQTINNSI LNEMVQVYAP IDRERIIEEL QKSGEVNDLI VSYHLLVDSK RGSYENEINS 370 380 390 400 410 420 PNLVSPITTP IMSSAQKSPI MFTTTTGFNP SNSNSISNNN NNNNNNNNNT TNNNNNTTNN 430 440 450 460 470 480 NNSIINNNNI NNNNINNNNN NNNNNINNNN IINNNNNNNN NNNNNNNNNN NNNNNNNNNS 490 500 510 520 530 540 SISGGTEVFS ISPNLNNSYN SNSSGNSNGS NSNNNSNNNT NNDNNNNNNN NNNNNNNNNN 550 560 570 580 590 600 NNNNNNNNNN CIDSVNNSLN NENDVNNSNI NNNNNNNSDD GSNNNSYEGG GDVLLLSDLN 610 620 630 640 650 660 GNNQLGGNDN GNVVNLNNNF QLLNSLDLNS DIQTQPHRKW YLGAISQLPP HEIMGEIYRA 670 680 690 700 710 720 LKKVGFEWKL TGPYQLRCRM VNGKPIKLVL QLFRVAENRY LLDIKKIEGE IFIFFDICSL MLEELNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Dictyostelium SNF1/AMP-activated kinase." Sung S., Bisson S., Koehler S., Podgorski G.J. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX2.
[3]	"Diverse cytopathologies in mitochondrial disease are caused by AMP-activated protein kinase signaling." Bokko P.B., Francione L., Bandala-Sanchez E., Ahmed A.U., Annesley S.J., Huang X., Khurana T., Kimmel A.R., Fisher P.R. Mol. Biol. Cell 18:1874-1886(2007) [PubMed: 17332500] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF118151 Genomic DNA. Translation: AAD30963.2. Frameshift. AAFI02000023 Genomic DNA. Translation: EAL68125.1.
RefSeq	XP_642250.1.
3D structure databases
HSSP	HSSP built from PDB template 2EUE based on UniProtKB P06782.
SMR	Q54YF2. Positions 28-292, 638-726.
ModBase	Search...
Genome annotation databases
GeneID	8621459.
KEGG	ddi:DDB_0215396.
Organism-specific databases
dictyBase	DDB_G0277905. snfA.
Phylogenomic databases
eggNOG	KOG0583.
HOGENOM	HBG755340.
OMA	TENGGTE.
PhylomeDB	Q54YF2.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR001772. Kinase-assoc_KA1. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR015741. Prot_kinase_Snf1-like_AMPK. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982:SF61. AMPK. 1 hit. PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
Pfam	PF02149. KA1. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS50032. KA1. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPKA_DICDI

Accession

Primary (citable) accession number: Q54YF2
Secondary accession number(s): Q9XYP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)