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Protein

Folic acid synthesis protein FOL1

Gene

fol1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes three sequential steps of tetrahydrofolate biosynthesis.By similarity

Catalytic activityi

7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde.By similarity
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.By similarity
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 3 and 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Folic acid synthesis protein FOL1 (fol1)
  4. Folic acid synthesis protein FOL1 (fol1)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Folic acid synthesis protein FOL1 (fol1)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi340MagnesiumBy similarity1
Binding sitei3806-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei4166-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei4356-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei5476-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei5836-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Lyase, Multifunctional enzyme, Transferase
Biological processFolate biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00154
UPA00077; UER00155
UPA00077; UER00156

Names & Taxonomyi

Protein namesi
Recommended name:
Folic acid synthesis protein FOL1
Including the following 3 domains:
Dihydroneopterin aldolase (EC:4.1.2.25)
Short name:
DHNA
Alternative name(s):
7,8-dihydroneopterin aldolase
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Short name:
HPPK
Alternative name(s):
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
PPPK
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Name:fol1
ORF Names:DDB_G0278283
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278283 fol1

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003281111 – 657Folic acid synthesis protein FOL1Add BLAST657

Proteomic databases

PaxDbiQ54YD9

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0230139

Structurei

3D structure databases

ProteinModelPortaliQ54YD9
SMRiQ54YD9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini333 – 641Pterin-bindingPROSITE-ProRule annotationAdd BLAST309

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 116DHNAAdd BLAST116
Regioni149 – 274HPPKAdd BLAST126
Regioni335 – 657DHPSAdd BLAST323
Regioni629 – 6316-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi303 – 306Poly-Pro4
Compositional biasi462 – 516Asn-richAdd BLAST55
Compositional biasi485 – 509Poly-AsnAdd BLAST25

Sequence similaritiesi

In the N-terminal section; belongs to the DHNA family.Curated
In the central section; belongs to the HPPK family.Curated
In the C-terminal section; belongs to the DHPS family.Curated

Phylogenomic databases

eggNOGiKOG2544 Eukaryota
COG0294 LUCA
COG0801 LUCA
InParanoidiQ54YD9
KOiK13939
OMAiSLWETEP
PhylomeDBiQ54YD9

Family and domain databases

CDDicd00739 DHPS, 1 hit
cd00483 HPPK, 1 hit
Gene3Di3.20.20.20, 1 hit
3.30.70.560, 1 hit
InterProiView protein in InterPro
IPR006390 DHP_synth
IPR011005 Dihydropteroate_synth-like
IPR006157 FolB_dom
IPR000550 Hppk
IPR035907 Hppk_sf
IPR000489 Pterin-binding_dom
PfamiView protein in Pfam
PF02152 FolB, 1 hit
PF01288 HPPK, 1 hit
PF00809 Pterin_bind, 1 hit
SMARTiView protein in SMART
SM00905 FolB, 1 hit
SUPFAMiSSF51717 SSF51717, 2 hits
SSF55083 SSF55083, 1 hit
TIGRFAMsiTIGR00526 folB_dom, 1 hit
TIGR01498 folK, 1 hit
PROSITEiView protein in PROSITE
PS00792 DHPS_1, 1 hit
PS00793 DHPS_2, 1 hit
PS00794 HPPK, 1 hit
PS50972 PTERIN_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q54YD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKIIIKDLL IQAVIGVNPG ERIIKQNIII SVTAYKDLSK CGSSDNVIDT
60 70 80 90 100
VSYSSLSKSI CSYSESSHHY TLEALATGVA KICCLGFGIE RVKVLVQKPG
110 120 130 140 150
AIKLAKWPGV QIERTLDYFK SNSFVEIPSK LINNNKNSNN SNAGNNIVYL
160 170 180 190 200
AFGSNLGDKF QNILNSFKRL EKQCFIQSTS FMYESSPQYY REQDSFYNCA
210 220 230 240 250
CKVSTDLKPH DLLKFIKQIE NDMGRVETFR NGPRVIDIDI IYYNGLIIKT
260 270 280 290 300
DDLEIPHPLM WERDFVLLPL SDIAPNFIHP TLHITTNRMK LNLPNGGNIH
310 320 330 340 350
NIPPPPTATT TCNNIIEKVI RIGNLNYNWN DKTFIMGILN VTPDSFVDGG
360 370 380 390 400
KFNTLEKSIQ QATALIEQGA DIIDIGGQST YPGAQQISIE EEINRVVPTI
410 420 430 440 450
KKIREVLGND IPLSIDTLHH QVAKEAILAG CNIINDVSGE FRVPIILNHS
460 470 480 490 500
QPTTQYLQQK QNEQYLNNSN DSNSNSSINT NGEDNNNNNN NNNNNNNNNN
510 520 530 540 550
NNNNNNNNND DNDNDNRSKI KQKIDLSSPK IETCTKLGLF RWQIILDPGL
560 570 580 590 600
GFYKTYEQSI EILQRGKELM GLGFPVLIGP SRKGFIANTI ANAEKDKSLP
610 620 630 640 650
PPSPKSERRL WGTIACCCIG SMWGANIIRI HDIPEIRDAM LISDSVNKPQ

RRYQIQK
Length:657
Mass (Da):73,720
Last modified:May 24, 2005 - v1
Checksum:iC1CA4397EE959845
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA Translation: EAL68314.1
RefSeqiXP_642263.1, XM_637171.1

Genome annotation databases

EnsemblProtistsiEAL68314; EAL68314; DDB_G0278283
GeneIDi8621472
KEGGiddi:DDB_G0278283

Similar proteinsi

Entry informationi

Entry nameiFOL1_DICDI
AccessioniPrimary (citable) accession number: Q54YD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health