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Q54YD9

- FOL1_DICDI

UniProt

Q54YD9 - FOL1_DICDI

Protein

Folic acid synthesis protein FOL1

Gene

fol1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalyzes three sequential steps of tetrahydrofolate biosynthesis.By similarity

    Catalytic activityi

    2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine = 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + glycolaldehyde.
    ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate.
    (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

    Cofactori

    Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi340 – 3401MagnesiumBy similarity
    Binding sitei348 – 3481SubstrateBy similarity
    Binding sitei416 – 4161SubstrateBy similarity
    Binding sitei435 – 4351SubstrateBy similarity
    Binding sitei547 – 5471SubstrateBy similarity
    Binding sitei583 – 5831SubstrateBy similarity
    Binding sitei629 – 6291SubstrateBy similarity
    Binding sitei631 – 6311SubstrateBy similarity

    GO - Molecular functioni

    1. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. dihydroneopterin aldolase activity Source: UniProtKB-EC
    4. dihydropteroate synthase activity Source: UniProtKB-EC
    5. kinase activity Source: UniProtKB-KW
    6. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Lyase, Transferase

    Keywords - Biological processi

    Folate biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00154.
    UPA00077; UER00155.
    UPA00077; UER00156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Folic acid synthesis protein FOL1
    Including the following 3 domains:
    Dihydroneopterin aldolase (EC:4.1.2.25)
    Short name:
    DHNA
    2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
    Alternative name(s):
    6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
    Short name:
    PPPK
    7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
    Short name:
    HPPK
    Dihydropteroate synthase (EC:2.5.1.15)
    Short name:
    DHPS
    Alternative name(s):
    Dihydropteroate pyrophosphorylase
    Gene namesi
    Name:fol1
    ORF Names:DDB_G0278283
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0278283. fol1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Folic acid synthesis protein FOL1PRO_0000328111Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0230139.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54YD9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini333 – 641309Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 116116DHNAAdd
    BLAST
    Regioni149 – 274126HPPKAdd
    BLAST
    Regioni325 – 657333DHPSAdd
    BLAST
    Regioni380 – 3812Substrate bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi303 – 3064Poly-Pro
    Compositional biasi462 – 51655Asn-richAdd
    BLAST
    Compositional biasi485 – 50925Poly-AsnAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the DHNA family.Curated
    In the central section; belongs to the HPPK family.Curated
    In the C-terminal section; belongs to the DHPS family.Curated
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0294.
    KOiK13939.
    OMAiWQIILDP.
    PhylomeDBiQ54YD9.

    Family and domain databases

    Gene3Di3.20.20.20. 1 hit.
    3.30.70.560. 1 hit.
    InterProiIPR011005. Dihydropteroate_synth-like.
    IPR006157. FolB_dom.
    IPR000550. Hppk.
    IPR000489. Pterin-binding.
    [Graphical view]
    PfamiPF02152. FolB. 1 hit.
    PF01288. HPPK. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    [Graphical view]
    SMARTiSM00905. FolB. 1 hit.
    [Graphical view]
    SUPFAMiSSF51717. SSF51717. 2 hits.
    SSF55083. SSF55083. 1 hit.
    TIGRFAMsiTIGR00526. folB_dom. 1 hit.
    TIGR01498. folK. 1 hit.
    PROSITEiPS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS00794. HPPK. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54YD9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKIIIKDLL IQAVIGVNPG ERIIKQNIII SVTAYKDLSK CGSSDNVIDT    50
    VSYSSLSKSI CSYSESSHHY TLEALATGVA KICCLGFGIE RVKVLVQKPG 100
    AIKLAKWPGV QIERTLDYFK SNSFVEIPSK LINNNKNSNN SNAGNNIVYL 150
    AFGSNLGDKF QNILNSFKRL EKQCFIQSTS FMYESSPQYY REQDSFYNCA 200
    CKVSTDLKPH DLLKFIKQIE NDMGRVETFR NGPRVIDIDI IYYNGLIIKT 250
    DDLEIPHPLM WERDFVLLPL SDIAPNFIHP TLHITTNRMK LNLPNGGNIH 300
    NIPPPPTATT TCNNIIEKVI RIGNLNYNWN DKTFIMGILN VTPDSFVDGG 350
    KFNTLEKSIQ QATALIEQGA DIIDIGGQST YPGAQQISIE EEINRVVPTI 400
    KKIREVLGND IPLSIDTLHH QVAKEAILAG CNIINDVSGE FRVPIILNHS 450
    QPTTQYLQQK QNEQYLNNSN DSNSNSSINT NGEDNNNNNN NNNNNNNNNN 500
    NNNNNNNNND DNDNDNRSKI KQKIDLSSPK IETCTKLGLF RWQIILDPGL 550
    GFYKTYEQSI EILQRGKELM GLGFPVLIGP SRKGFIANTI ANAEKDKSLP 600
    PPSPKSERRL WGTIACCCIG SMWGANIIRI HDIPEIRDAM LISDSVNKPQ 650
    RRYQIQK 657
    Length:657
    Mass (Da):73,720
    Last modified:May 24, 2005 - v1
    Checksum:iC1CA4397EE959845
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000023 Genomic DNA. Translation: EAL68314.1.
    RefSeqiXP_642263.1. XM_637171.1.

    Genome annotation databases

    EnsemblProtistsiDDB0230139; DDB0230139; DDB_G0278283.
    GeneIDi8621472.
    KEGGiddi:DDB_G0278283.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000023 Genomic DNA. Translation: EAL68314.1 .
    RefSeqi XP_642263.1. XM_637171.1.

    3D structure databases

    ProteinModelPortali Q54YD9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230139 ; DDB0230139 ; DDB_G0278283 .
    GeneIDi 8621472.
    KEGGi ddi:DDB_G0278283.

    Organism-specific databases

    dictyBasei DDB_G0278283. fol1.

    Phylogenomic databases

    eggNOGi COG0294.
    KOi K13939.
    OMAi WQIILDP.
    PhylomeDBi Q54YD9.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00154 .
    UPA00077 ; UER00155 .
    UPA00077 ; UER00156 .

    Family and domain databases

    Gene3Di 3.20.20.20. 1 hit.
    3.30.70.560. 1 hit.
    InterProi IPR011005. Dihydropteroate_synth-like.
    IPR006157. FolB_dom.
    IPR000550. Hppk.
    IPR000489. Pterin-binding.
    [Graphical view ]
    Pfami PF02152. FolB. 1 hit.
    PF01288. HPPK. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00905. FolB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51717. SSF51717. 2 hits.
    SSF55083. SSF55083. 1 hit.
    TIGRFAMsi TIGR00526. folB_dom. 1 hit.
    TIGR01498. folK. 1 hit.
    PROSITEi PS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS00794. HPPK. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiFOL1_DICDI
    AccessioniPrimary (citable) accession number: Q54YD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3