ID   SYIC_DICDI              Reviewed;        1067 AA.
AC   Q54YD4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   24-JAN-2024, entry version 128.
DE   RecName: Full=Probable isoleucine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.5;
DE   AltName: Full=Isoleucyl-tRNA synthetase;
DE            Short=IRS;
DE            Short=IleRS;
GN   Name=ileS; ORFNames=DDB_G0278293;
OS   Dictyostelium discoideum (Social amoeba).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC         isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC         Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC         ChEBI:CHEBI:456215; EC=6.1.1.5;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000023; EAL68319.1; -; Genomic_DNA.
DR   RefSeq; XP_642268.1; XM_637176.1.
DR   AlphaFoldDB; Q54YD4; -.
DR   SMR; Q54YD4; -.
DR   STRING; 44689.Q54YD4; -.
DR   PaxDb; 44689-DDB0231258; -.
DR   EnsemblProtists; EAL68319; EAL68319; DDB_G0278293.
DR   GeneID; 8621477; -.
DR   KEGG; ddi:DDB_G0278293; -.
DR   dictyBase; DDB_G0278293; ileS.
DR   eggNOG; KOG0434; Eukaryota.
DR   HOGENOM; CLU_001493_1_1_1; -.
DR   InParanoid; Q54YD4; -.
DR   OMA; EIIVIHK; -.
DR   PhylomeDB; Q54YD4; -.
DR   PRO; PR:Q54YD4; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005829; C:cytosol; ISS:dictyBase.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; ISS:dictyBase.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR   CDD; cd00818; IleRS_core; 1.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033709; Anticodon_Ile_ABEc.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   NCBIfam; TIGR00392; ileS; 1.
DR   PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR   PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF19302; DUF5915; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..1067
FT                   /note="Probable isoleucine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000327969"
FT   MOTIF           47..57
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000250"
FT   MOTIF           604..608
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000250"
FT   BINDING         607
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1067 AA;  122599 MW;  864E038EEE57243C CRC64;
     MEEVPQKIDF AGEESKILKY WDDIKAFETS VKMSEGKPEY SFYDGPPFAT GLPHYGHILA
     GTIKDTITRY AHQTGHHVER RFGWDCHGLP IEFEIDKLHG VRTKEDVLKM GIPTYNQHCR
     SIVMKYSHEW EIVVNRMGRW IDMKNNYKTM DTPFMESVWW VFQELFKKDL VYQGFKVMPY
     SIGCTTPLSN FEASSNYKDV SDPACVVSFQ TLDDEKVSIL AWTTTPWTLP SNLALTVNPK
     MEYIKINDIK RNQIFILGKN RTSILYKSDK EYTVLETMKG TDLIGKKYVP MFPYFASDAN
     MGGFVVIGGD FVTDDSGTGI VHTAPAYGED DFNVCIANGV ISRDQFKRPI LNSVDANGCF
     TSDVTDFAGM MVKDAETTKQ ISIYLKNKGR MVNSANLVHS YPYCWRSDTP LIYKAVGSWF
     VRVESIRDKL LANNDKTYWV PDFVKEKRFA NWLKNATDWA VSRNRYWGTP IPLWISEDGE
     EVVVIGSIDE LERLSGVRVT DLHRESIDHI TIPSQKGKGT LRRIEDVFDC WFESGSMPYA
     QQHYPFENKD KFEKIFPAHF IAEGLDQTRG WFYTLLVLST ALFDKPPFQN LIVNGLVLAA
     DGKKMSKRLK NYPDPMEVVS KVGADSLRLY LINSPVVRAE TLKFQEKGVQ DMIKDVFLPW
     FNAYRFFVQN CLRFEKATNT TFQPDIKVAL ASENVMDKWI LASCQSLIAF VRAEMAAYRL
     YTVVPKLVRF IEDLTNWFVR LNRKRLKGSN GDADCLAALN ILYEVLMTIC IAMGPFTPFF
     TEYMYQNLKK ALPKEKQMDS VHYVMFPEPI QEAFNTRIEE AISRMQVVIE LGRAARDRRT
     KPIKFPLKEF MVITENQQYL ADLESLKSYI LEELNIQNIV LTSDEGSFVV VTAEADNKRL
     GARLKNDFKK ISPLISKLTN EQLREFQKTQ TIDILGHELT SEDLKIIRKY NGETTNSEPS
     GNEEILTVLD LTVDSALYEK GLARELINRV QRLRKKSGLT FDDPVSMFYH TKEAELKTAI
     ENNNDYIKET ILFNLQFSET TSPSNSFATE IVSIVKDNDA EIYFLKN
//