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Q54YD4

- SYIC_DICDI

UniProt

Q54YD4 - SYIC_DICDI

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Protein

Probable isoleucine--tRNA ligase, cytoplasmic

Gene

ileS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei607 – 6071ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: dictyBase

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IRS
Short name:
IleRS
Gene namesi
Name:ileS
ORF Names:DDB_G0278293
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278293. ileS.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10671067Probable isoleucine--tRNA ligase, cytoplasmicPRO_0000327969Add
BLAST

Proteomic databases

PRIDEiQ54YD4.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231258.

Structurei

3D structure databases

ProteinModelPortaliQ54YD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionBy similarityAdd
BLAST
Motifi604 – 6085"KMSKS" regionBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
InParanoidiQ54YD4.
KOiK01870.
OMAiRVEHMVE.
PhylomeDBiQ54YD4.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54YD4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEVPQKIDF AGEESKILKY WDDIKAFETS VKMSEGKPEY SFYDGPPFAT
60 70 80 90 100
GLPHYGHILA GTIKDTITRY AHQTGHHVER RFGWDCHGLP IEFEIDKLHG
110 120 130 140 150
VRTKEDVLKM GIPTYNQHCR SIVMKYSHEW EIVVNRMGRW IDMKNNYKTM
160 170 180 190 200
DTPFMESVWW VFQELFKKDL VYQGFKVMPY SIGCTTPLSN FEASSNYKDV
210 220 230 240 250
SDPACVVSFQ TLDDEKVSIL AWTTTPWTLP SNLALTVNPK MEYIKINDIK
260 270 280 290 300
RNQIFILGKN RTSILYKSDK EYTVLETMKG TDLIGKKYVP MFPYFASDAN
310 320 330 340 350
MGGFVVIGGD FVTDDSGTGI VHTAPAYGED DFNVCIANGV ISRDQFKRPI
360 370 380 390 400
LNSVDANGCF TSDVTDFAGM MVKDAETTKQ ISIYLKNKGR MVNSANLVHS
410 420 430 440 450
YPYCWRSDTP LIYKAVGSWF VRVESIRDKL LANNDKTYWV PDFVKEKRFA
460 470 480 490 500
NWLKNATDWA VSRNRYWGTP IPLWISEDGE EVVVIGSIDE LERLSGVRVT
510 520 530 540 550
DLHRESIDHI TIPSQKGKGT LRRIEDVFDC WFESGSMPYA QQHYPFENKD
560 570 580 590 600
KFEKIFPAHF IAEGLDQTRG WFYTLLVLST ALFDKPPFQN LIVNGLVLAA
610 620 630 640 650
DGKKMSKRLK NYPDPMEVVS KVGADSLRLY LINSPVVRAE TLKFQEKGVQ
660 670 680 690 700
DMIKDVFLPW FNAYRFFVQN CLRFEKATNT TFQPDIKVAL ASENVMDKWI
710 720 730 740 750
LASCQSLIAF VRAEMAAYRL YTVVPKLVRF IEDLTNWFVR LNRKRLKGSN
760 770 780 790 800
GDADCLAALN ILYEVLMTIC IAMGPFTPFF TEYMYQNLKK ALPKEKQMDS
810 820 830 840 850
VHYVMFPEPI QEAFNTRIEE AISRMQVVIE LGRAARDRRT KPIKFPLKEF
860 870 880 890 900
MVITENQQYL ADLESLKSYI LEELNIQNIV LTSDEGSFVV VTAEADNKRL
910 920 930 940 950
GARLKNDFKK ISPLISKLTN EQLREFQKTQ TIDILGHELT SEDLKIIRKY
960 970 980 990 1000
NGETTNSEPS GNEEILTVLD LTVDSALYEK GLARELINRV QRLRKKSGLT
1010 1020 1030 1040 1050
FDDPVSMFYH TKEAELKTAI ENNNDYIKET ILFNLQFSET TSPSNSFATE
1060
IVSIVKDNDA EIYFLKN
Length:1,067
Mass (Da):122,599
Last modified:May 24, 2005 - v1
Checksum:i864E038EEE57243C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68319.1.
RefSeqiXP_642268.1. XM_637176.1.

Genome annotation databases

EnsemblProtistsiDDB0231258; DDB0231258; DDB_G0278293.
GeneIDi8621477.
KEGGiddi:DDB_G0278293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68319.1 .
RefSeqi XP_642268.1. XM_637176.1.

3D structure databases

ProteinModelPortali Q54YD4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231258.

Proteomic databases

PRIDEi Q54YD4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231258 ; DDB0231258 ; DDB_G0278293 .
GeneIDi 8621477.
KEGGi ddi:DDB_G0278293.

Organism-specific databases

dictyBasei DDB_G0278293. ileS.

Phylogenomic databases

eggNOGi COG0060.
InParanoidi Q54YD4.
KOi K01870.
OMAi RVEHMVE.
PhylomeDBi Q54YD4.

Miscellaneous databases

PROi Q54YD4.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiSYIC_DICDI
AccessioniPrimary (citable) accession number: Q54YD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3