Probable isoleucine--tRNA ligase, cytoplasmic - Dictyostelium discoideum (Slime mold)

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Q54YD4 (SYIC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable isoleucine--tRNA ligase, cytoplasmic
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IRS
Short name=IleRS

Gene names

Name:	ileS
ORF Names:	DDB_G0278293

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1067 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1067

1067

Probable isoleucine--tRNA ligase, cytoplasmic

PRO_0000327969

Regions

Motif

47 – 57

"HIGH" region By similarity

Motif

604 – 608

"KMSKS" region By similarity

Sites

Binding site

607

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q54YD4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 864E038EEE57243C
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FASTA

1,067

122,599

        10         20         30         40         50         60 
MEEVPQKIDF AGEESKILKY WDDIKAFETS VKMSEGKPEY SFYDGPPFAT GLPHYGHILA 

        70         80         90        100        110        120 
GTIKDTITRY AHQTGHHVER RFGWDCHGLP IEFEIDKLHG VRTKEDVLKM GIPTYNQHCR 

       130        140        150        160        170        180 
SIVMKYSHEW EIVVNRMGRW IDMKNNYKTM DTPFMESVWW VFQELFKKDL VYQGFKVMPY 

       190        200        210        220        230        240 
SIGCTTPLSN FEASSNYKDV SDPACVVSFQ TLDDEKVSIL AWTTTPWTLP SNLALTVNPK 

       250        260        270        280        290        300 
MEYIKINDIK RNQIFILGKN RTSILYKSDK EYTVLETMKG TDLIGKKYVP MFPYFASDAN 

       310        320        330        340        350        360 
MGGFVVIGGD FVTDDSGTGI VHTAPAYGED DFNVCIANGV ISRDQFKRPI LNSVDANGCF 

       370        380        390        400        410        420 
TSDVTDFAGM MVKDAETTKQ ISIYLKNKGR MVNSANLVHS YPYCWRSDTP LIYKAVGSWF 

       430        440        450        460        470        480 
VRVESIRDKL LANNDKTYWV PDFVKEKRFA NWLKNATDWA VSRNRYWGTP IPLWISEDGE 

       490        500        510        520        530        540 
EVVVIGSIDE LERLSGVRVT DLHRESIDHI TIPSQKGKGT LRRIEDVFDC WFESGSMPYA 

       550        560        570        580        590        600 
QQHYPFENKD KFEKIFPAHF IAEGLDQTRG WFYTLLVLST ALFDKPPFQN LIVNGLVLAA 

       610        620        630        640        650        660 
DGKKMSKRLK NYPDPMEVVS KVGADSLRLY LINSPVVRAE TLKFQEKGVQ DMIKDVFLPW 

       670        680        690        700        710        720 
FNAYRFFVQN CLRFEKATNT TFQPDIKVAL ASENVMDKWI LASCQSLIAF VRAEMAAYRL 

       730        740        750        760        770        780 
YTVVPKLVRF IEDLTNWFVR LNRKRLKGSN GDADCLAALN ILYEVLMTIC IAMGPFTPFF 

       790        800        810        820        830        840 
TEYMYQNLKK ALPKEKQMDS VHYVMFPEPI QEAFNTRIEE AISRMQVVIE LGRAARDRRT 

       850        860        870        880        890        900 
KPIKFPLKEF MVITENQQYL ADLESLKSYI LEELNIQNIV LTSDEGSFVV VTAEADNKRL 

       910        920        930        940        950        960 
GARLKNDFKK ISPLISKLTN EQLREFQKTQ TIDILGHELT SEDLKIIRKY NGETTNSEPS 

       970        980        990       1000       1010       1020 
GNEEILTVLD LTVDSALYEK GLARELINRV QRLRKKSGLT FDDPVSMFYH TKEAELKTAI 

      1030       1040       1050       1060 
ENNNDYIKET ILFNLQFSET TSPSNSFATE IVSIVKDNDA EIYFLKN

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References

[1]

"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.

Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000023 Genomic DNA. Translation: EAL68319.1.
RefSeq	XP_642268.1. XM_637176.1.
3D structure databases
HSSP	HSSP built from PDB template 1ILE based on UniProtKB P56690.
ProteinModelPortal	Q54YD4.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231258.
Proteomic databases
PRIDE	Q54YD4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231258; DDB0231258; DDB_G0278293.
GeneID	8621477.
KEGG	ddi:DDB_G0278293.
Organism-specific databases
dictyBase	DDB_G0278293. ileS.
Phylogenomic databases
eggNOG	COG0060.
KO	K01870.
OMA	SRSRYWG.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIC_DICDI

Accession

Primary (citable) accession number: Q54YD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents