Reviewed, UniProtKB/Swiss-Prot Q54YD4 (SYIC_DICDI)

Names and origin

Protein names

Recommended name:
    Probable isoleucyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS
      Short name=IRS

Gene names

Name:	ileS
ORF Names:	DDB_G0278293

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1067 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1067	1067	Probable isoleucyl-tRNA synthetase, cytoplasmic		PRO_0000327969
Regions
	Motif	47 – 57	11	"HIGH" region By similarity
	Motif	604 – 608	5	"KMSKS" region By similarity
Sites
	Binding site	607	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54YD4-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 864E038EEE57243C	FASTA	1,067	122,599
	10 20 30 40 50 60 MEEVPQKIDF AGEESKILKY WDDIKAFETS VKMSEGKPEY SFYDGPPFAT GLPHYGHILA 70 80 90 100 110 120 GTIKDTITRY AHQTGHHVER RFGWDCHGLP IEFEIDKLHG VRTKEDVLKM GIPTYNQHCR 130 140 150 160 170 180 SIVMKYSHEW EIVVNRMGRW IDMKNNYKTM DTPFMESVWW VFQELFKKDL VYQGFKVMPY 190 200 210 220 230 240 SIGCTTPLSN FEASSNYKDV SDPACVVSFQ TLDDEKVSIL AWTTTPWTLP SNLALTVNPK 250 260 270 280 290 300 MEYIKINDIK RNQIFILGKN RTSILYKSDK EYTVLETMKG TDLIGKKYVP MFPYFASDAN 310 320 330 340 350 360 MGGFVVIGGD FVTDDSGTGI VHTAPAYGED DFNVCIANGV ISRDQFKRPI LNSVDANGCF 370 380 390 400 410 420 TSDVTDFAGM MVKDAETTKQ ISIYLKNKGR MVNSANLVHS YPYCWRSDTP LIYKAVGSWF 430 440 450 460 470 480 VRVESIRDKL LANNDKTYWV PDFVKEKRFA NWLKNATDWA VSRNRYWGTP IPLWISEDGE 490 500 510 520 530 540 EVVVIGSIDE LERLSGVRVT DLHRESIDHI TIPSQKGKGT LRRIEDVFDC WFESGSMPYA 550 560 570 580 590 600 QQHYPFENKD KFEKIFPAHF IAEGLDQTRG WFYTLLVLST ALFDKPPFQN LIVNGLVLAA 610 620 630 640 650 660 DGKKMSKRLK NYPDPMEVVS KVGADSLRLY LINSPVVRAE TLKFQEKGVQ DMIKDVFLPW 670 680 690 700 710 720 FNAYRFFVQN CLRFEKATNT TFQPDIKVAL ASENVMDKWI LASCQSLIAF VRAEMAAYRL 730 740 750 760 770 780 YTVVPKLVRF IEDLTNWFVR LNRKRLKGSN GDADCLAALN ILYEVLMTIC IAMGPFTPFF 790 800 810 820 830 840 TEYMYQNLKK ALPKEKQMDS VHYVMFPEPI QEAFNTRIEE AISRMQVVIE LGRAARDRRT 850 860 870 880 890 900 KPIKFPLKEF MVITENQQYL ADLESLKSYI LEELNIQNIV LTSDEGSFVV VTAEADNKRL 910 920 930 940 950 960 GARLKNDFKK ISPLISKLTN EQLREFQKTQ TIDILGHELT SEDLKIIRKY NGETTNSEPS 970 980 990 1000 1010 1020 GNEEILTVLD LTVDSALYEK GLARELINRV QRLRKKSGLT FDDPVSMFYH TKEAELKTAI 1030 1040 1050 1060 ENNNDYIKET ILFNLQFSET TSPSNSFATE IVSIVKDNDA EIYFLKN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000023 Genomic DNA. Translation: EAL68319.1.
RefSeq	XP_642268.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54YD4.
Genome annotation databases
GeneID	3393337.
GenomeReviews	Gene locus ileS in contig CM000152_GR.
KEGG	ddi:DDB_0231258.
Organism-specific databases
dictyBase	DDB_G0278293. ileS.
Phylogenomic databases
OMA	SNWYVRR.
Enzyme and pathway databases
BRENDA	6.1.1.5. 424.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIC_DICDI

Accession

Primary (citable) accession number: Q54YD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)