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Q54YD4 (SYIC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable isoleucine--tRNA ligase, cytoplasmic

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IRS
Short name=IleRS
Gene names
Name:ileS
ORF Names:DDB_G0278293
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1067 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02003.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10671067Probable isoleucine--tRNA ligase, cytoplasmic HAMAP-Rule MF_02003
PRO_0000327969

Regions

Motif47 – 5711"HIGH" region By similarity
Motif604 – 6085"KMSKS" region By similarity

Sites

Binding site6071ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54YD4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 864E038EEE57243C

FASTA1,067122,599
        10         20         30         40         50         60 
MEEVPQKIDF AGEESKILKY WDDIKAFETS VKMSEGKPEY SFYDGPPFAT GLPHYGHILA 

        70         80         90        100        110        120 
GTIKDTITRY AHQTGHHVER RFGWDCHGLP IEFEIDKLHG VRTKEDVLKM GIPTYNQHCR 

       130        140        150        160        170        180 
SIVMKYSHEW EIVVNRMGRW IDMKNNYKTM DTPFMESVWW VFQELFKKDL VYQGFKVMPY 

       190        200        210        220        230        240 
SIGCTTPLSN FEASSNYKDV SDPACVVSFQ TLDDEKVSIL AWTTTPWTLP SNLALTVNPK 

       250        260        270        280        290        300 
MEYIKINDIK RNQIFILGKN RTSILYKSDK EYTVLETMKG TDLIGKKYVP MFPYFASDAN 

       310        320        330        340        350        360 
MGGFVVIGGD FVTDDSGTGI VHTAPAYGED DFNVCIANGV ISRDQFKRPI LNSVDANGCF 

       370        380        390        400        410        420 
TSDVTDFAGM MVKDAETTKQ ISIYLKNKGR MVNSANLVHS YPYCWRSDTP LIYKAVGSWF 

       430        440        450        460        470        480 
VRVESIRDKL LANNDKTYWV PDFVKEKRFA NWLKNATDWA VSRNRYWGTP IPLWISEDGE 

       490        500        510        520        530        540 
EVVVIGSIDE LERLSGVRVT DLHRESIDHI TIPSQKGKGT LRRIEDVFDC WFESGSMPYA 

       550        560        570        580        590        600 
QQHYPFENKD KFEKIFPAHF IAEGLDQTRG WFYTLLVLST ALFDKPPFQN LIVNGLVLAA 

       610        620        630        640        650        660 
DGKKMSKRLK NYPDPMEVVS KVGADSLRLY LINSPVVRAE TLKFQEKGVQ DMIKDVFLPW 

       670        680        690        700        710        720 
FNAYRFFVQN CLRFEKATNT TFQPDIKVAL ASENVMDKWI LASCQSLIAF VRAEMAAYRL 

       730        740        750        760        770        780 
YTVVPKLVRF IEDLTNWFVR LNRKRLKGSN GDADCLAALN ILYEVLMTIC IAMGPFTPFF 

       790        800        810        820        830        840 
TEYMYQNLKK ALPKEKQMDS VHYVMFPEPI QEAFNTRIEE AISRMQVVIE LGRAARDRRT 

       850        860        870        880        890        900 
KPIKFPLKEF MVITENQQYL ADLESLKSYI LEELNIQNIV LTSDEGSFVV VTAEADNKRL 

       910        920        930        940        950        960 
GARLKNDFKK ISPLISKLTN EQLREFQKTQ TIDILGHELT SEDLKIIRKY NGETTNSEPS 

       970        980        990       1000       1010       1020 
GNEEILTVLD LTVDSALYEK GLARELINRV QRLRKKSGLT FDDPVSMFYH TKEAELKTAI 

      1030       1040       1050       1060 
ENNNDYIKET ILFNLQFSET TSPSNSFATE IVSIVKDNDA EIYFLKN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000023 Genomic DNA. Translation: EAL68319.1.

3D structure databases

ProteinModelPortalQ54YD4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231258.

Proteomic databases

PRIDEQ54YD4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231258; DDB0231258; DDB_G0278293.
KEGGddi:DDB_G0278293.

Organism-specific databases

dictyBaseDDB_G0278293. ileS.

Phylogenomic databases

eggNOGCOG0060.
KOK01870.
OMADWNLSRS.
PhylomeDBQ54YD4.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54YD4.

Entry information

Entry nameSYIC_DICDI
AccessionPrimary (citable) accession number: Q54YD4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries