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Q54YD4

- SYIC_DICDI

UniProt

Q54YD4 - SYIC_DICDI

Protein

Probable isoleucine--tRNA ligase, cytoplasmic

Gene

ileS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei607 – 6071ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: dictyBase

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IRS
    Short name:
    IleRS
    Gene namesi
    Name:ileS
    ORF Names:DDB_G0278293
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0278293. ileS.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10671067Probable isoleucine--tRNA ligase, cytoplasmicPRO_0000327969Add
    BLAST

    Proteomic databases

    PRIDEiQ54YD4.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231258.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54YD4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionBy similarityAdd
    BLAST
    Motifi604 – 6085"KMSKS" regionBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiRVEHMVE.
    PhylomeDBiQ54YD4.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54YD4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEVPQKIDF AGEESKILKY WDDIKAFETS VKMSEGKPEY SFYDGPPFAT     50
    GLPHYGHILA GTIKDTITRY AHQTGHHVER RFGWDCHGLP IEFEIDKLHG 100
    VRTKEDVLKM GIPTYNQHCR SIVMKYSHEW EIVVNRMGRW IDMKNNYKTM 150
    DTPFMESVWW VFQELFKKDL VYQGFKVMPY SIGCTTPLSN FEASSNYKDV 200
    SDPACVVSFQ TLDDEKVSIL AWTTTPWTLP SNLALTVNPK MEYIKINDIK 250
    RNQIFILGKN RTSILYKSDK EYTVLETMKG TDLIGKKYVP MFPYFASDAN 300
    MGGFVVIGGD FVTDDSGTGI VHTAPAYGED DFNVCIANGV ISRDQFKRPI 350
    LNSVDANGCF TSDVTDFAGM MVKDAETTKQ ISIYLKNKGR MVNSANLVHS 400
    YPYCWRSDTP LIYKAVGSWF VRVESIRDKL LANNDKTYWV PDFVKEKRFA 450
    NWLKNATDWA VSRNRYWGTP IPLWISEDGE EVVVIGSIDE LERLSGVRVT 500
    DLHRESIDHI TIPSQKGKGT LRRIEDVFDC WFESGSMPYA QQHYPFENKD 550
    KFEKIFPAHF IAEGLDQTRG WFYTLLVLST ALFDKPPFQN LIVNGLVLAA 600
    DGKKMSKRLK NYPDPMEVVS KVGADSLRLY LINSPVVRAE TLKFQEKGVQ 650
    DMIKDVFLPW FNAYRFFVQN CLRFEKATNT TFQPDIKVAL ASENVMDKWI 700
    LASCQSLIAF VRAEMAAYRL YTVVPKLVRF IEDLTNWFVR LNRKRLKGSN 750
    GDADCLAALN ILYEVLMTIC IAMGPFTPFF TEYMYQNLKK ALPKEKQMDS 800
    VHYVMFPEPI QEAFNTRIEE AISRMQVVIE LGRAARDRRT KPIKFPLKEF 850
    MVITENQQYL ADLESLKSYI LEELNIQNIV LTSDEGSFVV VTAEADNKRL 900
    GARLKNDFKK ISPLISKLTN EQLREFQKTQ TIDILGHELT SEDLKIIRKY 950
    NGETTNSEPS GNEEILTVLD LTVDSALYEK GLARELINRV QRLRKKSGLT 1000
    FDDPVSMFYH TKEAELKTAI ENNNDYIKET ILFNLQFSET TSPSNSFATE 1050
    IVSIVKDNDA EIYFLKN 1067
    Length:1,067
    Mass (Da):122,599
    Last modified:May 24, 2005 - v1
    Checksum:i864E038EEE57243C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000023 Genomic DNA. Translation: EAL68319.1.
    RefSeqiXP_642268.1. XM_637176.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231258; DDB0231258; DDB_G0278293.
    GeneIDi8621477.
    KEGGiddi:DDB_G0278293.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000023 Genomic DNA. Translation: EAL68319.1 .
    RefSeqi XP_642268.1. XM_637176.1.

    3D structure databases

    ProteinModelPortali Q54YD4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231258.

    Proteomic databases

    PRIDEi Q54YD4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231258 ; DDB0231258 ; DDB_G0278293 .
    GeneIDi 8621477.
    KEGGi ddi:DDB_G0278293.

    Organism-specific databases

    dictyBasei DDB_G0278293. ileS.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi RVEHMVE.
    PhylomeDBi Q54YD4.

    Miscellaneous databases

    PROi Q54YD4.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiSYIC_DICDI
    AccessioniPrimary (citable) accession number: Q54YD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3