Alpha-mannosidase D precursor - Dictyostelium discoideum (Slime mold)

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Q54YC4 (MAND_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 56. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-mannosidase D
EC=3.2.1.24

Gene names

Name:	manD
ORF Names:	DDB_G0278653

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-mannosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

21

Potential

Chain

1201

Alpha-mannosidase D

PRO_0000327843

Regions

Topological domain

1149

Extracellular Potential

Transmembrane

21

Helical; Potential

Topological domain

31

Cytoplasmic Potential

Compositional bias

24

Poly-Asn

Compositional bias

10

Poly-Ser

Compositional bias

5

Poly-Asn

Compositional bias

7

Poly-Asn

Sites

Active site

1

Nucleophile By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q54YC4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: FDA5A919C2C65880
Show »

1,222

141,190

        10         20         30         40         50         60 
MESSKFVKII WVFGIWILVF TFLIIYNNYD YKSTFGINKF EKRSLKTINQ INNKENGDDK 

        70         80         90        100        110        120 
KLSVFLIPHS HCDGGWLQDY DGYYYNIVQY ILSGVVNELN LDKEKKFNWV EIGFFSRWWN 

       130        140        150        160        170        180 
DQNEIQKEIV RNLINNKQLS FISGGWVQND EATATIDDVI TQMTQGHQWL KDTLNYTVEY 

       190        200        210        220        230        240 
AWQIDPFGYS SSTPTIFSSM GIKGLVINRV SNDVKSYMKS VKEMEFIWKG SESLGEQSQM 

       250        260        270        280        290        300 
LVSTLNIHYD YPKHIDPKKD FSLNDRIKGF TKYLNDLSKT RESNILMIPL GDDFRYSNAK 

       310        320        330        340        350        360 
TEFSVSKEWV KALQDNKEYY NIKEIKYATL DEYFIALEDS FLRNNKFGKS RKQNVYTETF 

       370        380        390        400        410        420 
SDGDDEVSAL SLYNKDFFPY STGNLEYWTG YYTTRPLLKR LIRESSLLQK SSDILYTLAI 

       430        440        450        460        470        480 
GENSNNQIDI NNLQTLSNQL NENRNTIALV QHHDIVTGTS RSFVLNDNFQ RLQKSRISNY 

       490        500        510        520        530        540 
NIISNSLEYL LNKSNNKTNN NNNNNNKNNN NNNNNNNNNN NNLKNTNSIS TTGSSSSSGS 

       550        560        570        580        590        600 
GSSNNNNNTV NKSEPFNFEN AIDLSNESNN QYSLVFHNTL GWEVNQHVSF RIKVNKNDNQ 

       610        620        630        640        650        660 
LLESIQLVEA ISNKTIQIQI IPIQDDSNCQ SIENNYIVFA IINLPPLGLN TYYLSIANSE 

       670        680        690        700        710        720 
DSKSNFTYLS KPKLLKKGNE INFNNNRFKV EFESNGLISK ITDKNSNEIK TIEQTFHQYS 

       730        740        750        760        770        780 
TKKSGPYIFN VKGGKKHGFL ENPDKFIYHD GPLVSQLTML YGVDDYCNVT SIVVQRIYKN 

       790        800        810        820        830        840 
NNEINNSNSK SLITENYIET GYSINGDMNR ETTINYKVKD LENDDIFYTD NGLESRKRIY 

       850        860        870        880        890        900 
NHDRSVNQNY YPVLGYIKLK ETSENNNHQY TVYVDRSVGA TSPSDGEMEI MIHRTMDTDD 

       910        920        930        940        950        960 
WKGVNWPSKD IGRSDAKLYF NMDLVNNQLQ NEKRISLHIT NQPIMFVKKH NNQTGYLLKY 

       970        980        990       1000       1010       1020 
SPLSNQLPSN IHLQSLLTLK NNTVGLRLFN IHEVDSNTQS TTDTDKSTLF NELEISNFIE 

      1030       1040       1050       1060       1070       1080 
TGLSFLKLTK NNLIDKFSTR INKKFPIKCG ESEYSFINEK PSSIIFSNNG TNNIIDGENK 

      1090       1100       1110       1120       1130       1140 
GENNKTIETI QIKPHEIKSF TFNFNFQLDQ IIPNNNNNNN KYAINKELNN EYIEQSIENQ 

      1150       1160       1170       1180       1190       1200 
RYEYDFSFFP IKPTFYDDRG KYNRPNHLAL ILSLSIGIPA GILIIVIALV VTYKKRKNRK 

      1210       1220 
TLTSSNSSSN LIQQEDQTDY SP

References

[1]

"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.

expand/collapse author list

Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000023 Genomic DNA. Translation: EAL68499.1.
RefSeq	XP_642278.1. XM_637186.1.
3D structure databases
ProteinModelPortal	Q54YC4.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231614.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231614; DDB0231614; DDB_G0278653.
GeneID	8621485.
KEGG	ddi:DDB_G0278653.
Organism-specific databases
dictyBase	DDB_G0278653. manD.
Phylogenomic databases
eggNOG	COG0383.
OMA	FISGGWV.
ProtClustDB	CLSZ2430771.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.110.10. 1 hit.
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR013780. Glyco_hydro_13_b. IPR027291. Glyco_hydro_38/57_N. IPR015341. Glyco_hydro_38_cen_dom. IPR000602. Glyco_hydro_38_N. [Graphical view]
Pfam	PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. [Graphical view]
SMART	SM00872. Alpha-mann_mid. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MAND_DICDI

Accession

Primary (citable) accession number: Q54YC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 29, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents