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Q54YC4

- MAND_DICDI

UniProt

Q54YC4 - MAND_DICDI

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Protein
Alpha-mannosidase D
Gene
manD, DDB_G0278653
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi71 – 711Zinc By similarity
Metal bindingi73 – 731Zinc By similarity
Active sitei185 – 1851Nucleophile By similarity
Metal bindingi185 – 1851Zinc By similarity
Metal bindingi453 – 4531Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase D (EC:3.2.1.24)
Gene namesi
Name:manD
ORF Names:DDB_G0278653
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278653. manD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 11701149Extracellular Reviewed prediction
Add
BLAST
Transmembranei1171 – 119121Helical; Reviewed prediction
Add
BLAST
Topological domaini1192 – 122231Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 12221201Alpha-mannosidase D
PRO_0000327843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi175 – 1751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi492 – 4921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi496 – 4961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi547 – 5471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi551 – 5511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi566 – 5661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi613 – 6131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi665 – 6651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi768 – 7681N-linked (GlcNAc...) Reviewed prediction
Glycosylationi785 – 7851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi952 – 9521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi981 – 9811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1069 – 10691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1084 – 10841N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231614.

Structurei

3D structure databases

ProteinModelPortaliQ54YC4.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi499 – 52224Poly-Asn
Add
BLAST
Compositional biasi534 – 54310Poly-Ser
Compositional biasi544 – 5485Poly-Asn
Compositional biasi1114 – 11207Poly-Asn

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0383.
OMAiFISGGWV.
PhylomeDBiQ54YC4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YC4-1 [UniParc]FASTAAdd to Basket

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MESSKFVKII WVFGIWILVF TFLIIYNNYD YKSTFGINKF EKRSLKTINQ     50
INNKENGDDK KLSVFLIPHS HCDGGWLQDY DGYYYNIVQY ILSGVVNELN 100
LDKEKKFNWV EIGFFSRWWN DQNEIQKEIV RNLINNKQLS FISGGWVQND 150
EATATIDDVI TQMTQGHQWL KDTLNYTVEY AWQIDPFGYS SSTPTIFSSM 200
GIKGLVINRV SNDVKSYMKS VKEMEFIWKG SESLGEQSQM LVSTLNIHYD 250
YPKHIDPKKD FSLNDRIKGF TKYLNDLSKT RESNILMIPL GDDFRYSNAK 300
TEFSVSKEWV KALQDNKEYY NIKEIKYATL DEYFIALEDS FLRNNKFGKS 350
RKQNVYTETF SDGDDEVSAL SLYNKDFFPY STGNLEYWTG YYTTRPLLKR 400
LIRESSLLQK SSDILYTLAI GENSNNQIDI NNLQTLSNQL NENRNTIALV 450
QHHDIVTGTS RSFVLNDNFQ RLQKSRISNY NIISNSLEYL LNKSNNKTNN 500
NNNNNNKNNN NNNNNNNNNN NNLKNTNSIS TTGSSSSSGS GSSNNNNNTV 550
NKSEPFNFEN AIDLSNESNN QYSLVFHNTL GWEVNQHVSF RIKVNKNDNQ 600
LLESIQLVEA ISNKTIQIQI IPIQDDSNCQ SIENNYIVFA IINLPPLGLN 650
TYYLSIANSE DSKSNFTYLS KPKLLKKGNE INFNNNRFKV EFESNGLISK 700
ITDKNSNEIK TIEQTFHQYS TKKSGPYIFN VKGGKKHGFL ENPDKFIYHD 750
GPLVSQLTML YGVDDYCNVT SIVVQRIYKN NNEINNSNSK SLITENYIET 800
GYSINGDMNR ETTINYKVKD LENDDIFYTD NGLESRKRIY NHDRSVNQNY 850
YPVLGYIKLK ETSENNNHQY TVYVDRSVGA TSPSDGEMEI MIHRTMDTDD 900
WKGVNWPSKD IGRSDAKLYF NMDLVNNQLQ NEKRISLHIT NQPIMFVKKH 950
NNQTGYLLKY SPLSNQLPSN IHLQSLLTLK NNTVGLRLFN IHEVDSNTQS 1000
TTDTDKSTLF NELEISNFIE TGLSFLKLTK NNLIDKFSTR INKKFPIKCG 1050
ESEYSFINEK PSSIIFSNNG TNNIIDGENK GENNKTIETI QIKPHEIKSF 1100
TFNFNFQLDQ IIPNNNNNNN KYAINKELNN EYIEQSIENQ RYEYDFSFFP 1150
IKPTFYDDRG KYNRPNHLAL ILSLSIGIPA GILIIVIALV VTYKKRKNRK 1200
TLTSSNSSSN LIQQEDQTDY SP 1222
Length:1,222
Mass (Da):141,190
Last modified:May 24, 2005 - v1
Checksum:iFDA5A919C2C65880
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68499.1.
RefSeqiXP_642278.1. XM_637186.1.

Genome annotation databases

EnsemblProtistsiDDB0231614; DDB0231614; DDB_G0278653.
GeneIDi8621485.
KEGGiddi:DDB_G0278653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68499.1 .
RefSeqi XP_642278.1. XM_637186.1.

3D structure databases

ProteinModelPortali Q54YC4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231614.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231614 ; DDB0231614 ; DDB_G0278653 .
GeneIDi 8621485.
KEGGi ddi:DDB_G0278653.

Organism-specific databases

dictyBasei DDB_G0278653. manD.

Phylogenomic databases

eggNOGi COG0383.
OMAi FISGGWV.
PhylomeDBi Q54YC4.

Enzyme and pathway databases

Reactomei REACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMAND_DICDI
AccessioniPrimary (citable) accession number: Q54YC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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