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Protein

Alpha-mannosidase D

Gene

manD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi71ZincBy similarity1
Metal bindingi73ZincBy similarity1
Active sitei185NucleophileBy similarity1
Metal bindingi185ZincBy similarity1
Metal bindingi453ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase D (EC:3.2.1.24)
Gene namesi
Name:manD
ORF Names:DDB_G0278653
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278653. manD.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1170ExtracellularSequence analysisAdd BLAST1149
Transmembranei1171 – 1191HelicalSequence analysisAdd BLAST21
Topological domaini1192 – 1222CytoplasmicSequence analysisAdd BLAST31

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000032784322 – 1222Alpha-mannosidase DAdd BLAST1201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi175N-linked (GlcNAc...)Sequence analysis1
Glycosylationi492N-linked (GlcNAc...)Sequence analysis1
Glycosylationi496N-linked (GlcNAc...)Sequence analysis1
Glycosylationi547N-linked (GlcNAc...)Sequence analysis1
Glycosylationi551N-linked (GlcNAc...)Sequence analysis1
Glycosylationi566N-linked (GlcNAc...)Sequence analysis1
Glycosylationi613N-linked (GlcNAc...)Sequence analysis1
Glycosylationi665N-linked (GlcNAc...)Sequence analysis1
Glycosylationi768N-linked (GlcNAc...)Sequence analysis1
Glycosylationi785N-linked (GlcNAc...)Sequence analysis1
Glycosylationi952N-linked (GlcNAc...)Sequence analysis1
Glycosylationi981N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1069N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1084N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ54YC4.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231614.

Structurei

3D structure databases

ProteinModelPortaliQ54YC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi499 – 522Poly-AsnAdd BLAST24
Compositional biasi534 – 543Poly-Ser10
Compositional biasi544 – 548Poly-Asn5
Compositional biasi1114 – 1120Poly-Asn7

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1958. Eukaryota.
KOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ54YC4.
OMAiLFLHHDA.
PhylomeDBiQ54YC4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSKFVKII WVFGIWILVF TFLIIYNNYD YKSTFGINKF EKRSLKTINQ
60 70 80 90 100
INNKENGDDK KLSVFLIPHS HCDGGWLQDY DGYYYNIVQY ILSGVVNELN
110 120 130 140 150
LDKEKKFNWV EIGFFSRWWN DQNEIQKEIV RNLINNKQLS FISGGWVQND
160 170 180 190 200
EATATIDDVI TQMTQGHQWL KDTLNYTVEY AWQIDPFGYS SSTPTIFSSM
210 220 230 240 250
GIKGLVINRV SNDVKSYMKS VKEMEFIWKG SESLGEQSQM LVSTLNIHYD
260 270 280 290 300
YPKHIDPKKD FSLNDRIKGF TKYLNDLSKT RESNILMIPL GDDFRYSNAK
310 320 330 340 350
TEFSVSKEWV KALQDNKEYY NIKEIKYATL DEYFIALEDS FLRNNKFGKS
360 370 380 390 400
RKQNVYTETF SDGDDEVSAL SLYNKDFFPY STGNLEYWTG YYTTRPLLKR
410 420 430 440 450
LIRESSLLQK SSDILYTLAI GENSNNQIDI NNLQTLSNQL NENRNTIALV
460 470 480 490 500
QHHDIVTGTS RSFVLNDNFQ RLQKSRISNY NIISNSLEYL LNKSNNKTNN
510 520 530 540 550
NNNNNNKNNN NNNNNNNNNN NNLKNTNSIS TTGSSSSSGS GSSNNNNNTV
560 570 580 590 600
NKSEPFNFEN AIDLSNESNN QYSLVFHNTL GWEVNQHVSF RIKVNKNDNQ
610 620 630 640 650
LLESIQLVEA ISNKTIQIQI IPIQDDSNCQ SIENNYIVFA IINLPPLGLN
660 670 680 690 700
TYYLSIANSE DSKSNFTYLS KPKLLKKGNE INFNNNRFKV EFESNGLISK
710 720 730 740 750
ITDKNSNEIK TIEQTFHQYS TKKSGPYIFN VKGGKKHGFL ENPDKFIYHD
760 770 780 790 800
GPLVSQLTML YGVDDYCNVT SIVVQRIYKN NNEINNSNSK SLITENYIET
810 820 830 840 850
GYSINGDMNR ETTINYKVKD LENDDIFYTD NGLESRKRIY NHDRSVNQNY
860 870 880 890 900
YPVLGYIKLK ETSENNNHQY TVYVDRSVGA TSPSDGEMEI MIHRTMDTDD
910 920 930 940 950
WKGVNWPSKD IGRSDAKLYF NMDLVNNQLQ NEKRISLHIT NQPIMFVKKH
960 970 980 990 1000
NNQTGYLLKY SPLSNQLPSN IHLQSLLTLK NNTVGLRLFN IHEVDSNTQS
1010 1020 1030 1040 1050
TTDTDKSTLF NELEISNFIE TGLSFLKLTK NNLIDKFSTR INKKFPIKCG
1060 1070 1080 1090 1100
ESEYSFINEK PSSIIFSNNG TNNIIDGENK GENNKTIETI QIKPHEIKSF
1110 1120 1130 1140 1150
TFNFNFQLDQ IIPNNNNNNN KYAINKELNN EYIEQSIENQ RYEYDFSFFP
1160 1170 1180 1190 1200
IKPTFYDDRG KYNRPNHLAL ILSLSIGIPA GILIIVIALV VTYKKRKNRK
1210 1220
TLTSSNSSSN LIQQEDQTDY SP
Length:1,222
Mass (Da):141,190
Last modified:May 24, 2005 - v1
Checksum:iFDA5A919C2C65880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68499.1.
RefSeqiXP_642278.1. XM_637186.1.

Genome annotation databases

EnsemblProtistsiEAL68499; EAL68499; DDB_G0278653.
GeneIDi8621485.
KEGGiddi:DDB_G0278653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68499.1.
RefSeqiXP_642278.1. XM_637186.1.

3D structure databases

ProteinModelPortaliQ54YC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231614.

Proteomic databases

PaxDbiQ54YC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL68499; EAL68499; DDB_G0278653.
GeneIDi8621485.
KEGGiddi:DDB_G0278653.

Organism-specific databases

dictyBaseiDDB_G0278653. manD.

Phylogenomic databases

eggNOGiKOG1958. Eukaryota.
KOG1959. Eukaryota.
ENOG410XQMZ. LUCA.
InParanoidiQ54YC4.
OMAiLFLHHDA.
PhylomeDBiQ54YC4.

Enzyme and pathway databases

ReactomeiR-DDI-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

PROiQ54YC4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR013780. Glyco_hydro_b.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAND_DICDI
AccessioniPrimary (citable) accession number: Q54YC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.