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Q54YC4

- MAND_DICDI

UniProt

Q54YC4 - MAND_DICDI

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Protein

Alpha-mannosidase D

Gene

manD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi71 – 711ZincBy similarity
Metal bindingi73 – 731ZincBy similarity
Active sitei185 – 1851NucleophileBy similarity
Metal bindingi185 – 1851ZincBy similarity
Metal bindingi453 – 4531ZincBy similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase D (EC:3.2.1.24)
Gene namesi
Name:manD
ORF Names:DDB_G0278653
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278653. manD.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 12221201Alpha-mannosidase DPRO_0000327843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi496 – 4961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi547 – 5471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi566 – 5661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi613 – 6131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi665 – 6651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi768 – 7681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi785 – 7851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi952 – 9521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi981 – 9811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1069 – 10691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1084 – 10841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231614.

Structurei

3D structure databases

ProteinModelPortaliQ54YC4.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 11701149ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1192 – 122231CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1171 – 119121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi499 – 52224Poly-AsnAdd
BLAST
Compositional biasi534 – 54310Poly-Ser
Compositional biasi544 – 5485Poly-Asn
Compositional biasi1114 – 11207Poly-Asn

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0383.
InParanoidiQ54YC4.
OMAiFISGGWV.
PhylomeDBiQ54YC4.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54YC4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESSKFVKII WVFGIWILVF TFLIIYNNYD YKSTFGINKF EKRSLKTINQ
60 70 80 90 100
INNKENGDDK KLSVFLIPHS HCDGGWLQDY DGYYYNIVQY ILSGVVNELN
110 120 130 140 150
LDKEKKFNWV EIGFFSRWWN DQNEIQKEIV RNLINNKQLS FISGGWVQND
160 170 180 190 200
EATATIDDVI TQMTQGHQWL KDTLNYTVEY AWQIDPFGYS SSTPTIFSSM
210 220 230 240 250
GIKGLVINRV SNDVKSYMKS VKEMEFIWKG SESLGEQSQM LVSTLNIHYD
260 270 280 290 300
YPKHIDPKKD FSLNDRIKGF TKYLNDLSKT RESNILMIPL GDDFRYSNAK
310 320 330 340 350
TEFSVSKEWV KALQDNKEYY NIKEIKYATL DEYFIALEDS FLRNNKFGKS
360 370 380 390 400
RKQNVYTETF SDGDDEVSAL SLYNKDFFPY STGNLEYWTG YYTTRPLLKR
410 420 430 440 450
LIRESSLLQK SSDILYTLAI GENSNNQIDI NNLQTLSNQL NENRNTIALV
460 470 480 490 500
QHHDIVTGTS RSFVLNDNFQ RLQKSRISNY NIISNSLEYL LNKSNNKTNN
510 520 530 540 550
NNNNNNKNNN NNNNNNNNNN NNLKNTNSIS TTGSSSSSGS GSSNNNNNTV
560 570 580 590 600
NKSEPFNFEN AIDLSNESNN QYSLVFHNTL GWEVNQHVSF RIKVNKNDNQ
610 620 630 640 650
LLESIQLVEA ISNKTIQIQI IPIQDDSNCQ SIENNYIVFA IINLPPLGLN
660 670 680 690 700
TYYLSIANSE DSKSNFTYLS KPKLLKKGNE INFNNNRFKV EFESNGLISK
710 720 730 740 750
ITDKNSNEIK TIEQTFHQYS TKKSGPYIFN VKGGKKHGFL ENPDKFIYHD
760 770 780 790 800
GPLVSQLTML YGVDDYCNVT SIVVQRIYKN NNEINNSNSK SLITENYIET
810 820 830 840 850
GYSINGDMNR ETTINYKVKD LENDDIFYTD NGLESRKRIY NHDRSVNQNY
860 870 880 890 900
YPVLGYIKLK ETSENNNHQY TVYVDRSVGA TSPSDGEMEI MIHRTMDTDD
910 920 930 940 950
WKGVNWPSKD IGRSDAKLYF NMDLVNNQLQ NEKRISLHIT NQPIMFVKKH
960 970 980 990 1000
NNQTGYLLKY SPLSNQLPSN IHLQSLLTLK NNTVGLRLFN IHEVDSNTQS
1010 1020 1030 1040 1050
TTDTDKSTLF NELEISNFIE TGLSFLKLTK NNLIDKFSTR INKKFPIKCG
1060 1070 1080 1090 1100
ESEYSFINEK PSSIIFSNNG TNNIIDGENK GENNKTIETI QIKPHEIKSF
1110 1120 1130 1140 1150
TFNFNFQLDQ IIPNNNNNNN KYAINKELNN EYIEQSIENQ RYEYDFSFFP
1160 1170 1180 1190 1200
IKPTFYDDRG KYNRPNHLAL ILSLSIGIPA GILIIVIALV VTYKKRKNRK
1210 1220
TLTSSNSSSN LIQQEDQTDY SP
Length:1,222
Mass (Da):141,190
Last modified:May 24, 2005 - v1
Checksum:iFDA5A919C2C65880
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68499.1.
RefSeqiXP_642278.1. XM_637186.1.

Genome annotation databases

EnsemblProtistsiDDB0231614; DDB0231614; DDB_G0278653.
GeneIDi8621485.
KEGGiddi:DDB_G0278653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000023 Genomic DNA. Translation: EAL68499.1 .
RefSeqi XP_642278.1. XM_637186.1.

3D structure databases

ProteinModelPortali Q54YC4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231614.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231614 ; DDB0231614 ; DDB_G0278653 .
GeneIDi 8621485.
KEGGi ddi:DDB_G0278653.

Organism-specific databases

dictyBasei DDB_G0278653. manD.

Phylogenomic databases

eggNOGi COG0383.
InParanoidi Q54YC4.
OMAi FISGGWV.
PhylomeDBi Q54YC4.

Enzyme and pathway databases

Reactomei REACT_221646. Reactions specific to the complex N-glycan synthesis pathway.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 2 hits.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMAND_DICDI
AccessioniPrimary (citable) accession number: Q54YC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3