Q54Y32 (MPL3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAP kinase phosphatase with leucine-rich repeats protein 3
EC=3.1.3.16
EC=3.1.3.48

Gene names

Name:	mpl3
ORF Names:	DDB_G0278445

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probable phosphatase with dual specificity toward Ser/Thr and Tyr-containing proteins By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 8 LRR (leucine-rich) repeats. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Domain	Leucine-rich repeat Repeat
Molecular function	Hydrolase Protein phosphatase
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 856	855	MAP kinase phosphatase with leucine-rich repeats protein 3		PRO_0000332957
Regions
	Repeat	344 – 365	22	LRR 1
	Repeat	370 – 391	22	LRR 2
	Repeat	392 – 413	22	LRR 3
	Repeat	416 – 437	22	LRR 4
	Repeat	439 – 461	23	LRR 5
	Repeat	462 – 484	23	LRR 6
	Repeat	485 – 506	22	LRR 7
	Repeat	507 – 528	22	LRR 8
	Domain	632 – 772	141	Tyrosine-protein phosphatase
	Compositional bias	14 – 25	12	Poly-Gly
	Compositional bias	93 – 107	15	Poly-Asn
	Compositional bias	116 – 130	15	Poly-Thr
	Compositional bias	137 – 142	6	Poly-Gln
	Compositional bias	223 – 230	8	Poly-Gln
	Compositional bias	563 – 583	21	Poly-Asn
	Compositional bias	815 – 820	6	Poly-Asn
	Compositional bias	837 – 850	14	Poly-Ser
Sites
	Active site	717	1	Phosphocysteine intermediate By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54Y32 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: FA899072865A6CEA	FASTA	856	95,418
	10 20 30 40 50 60 MGNSHSSENG GNSGSGGGSG GGGSGYSGVS ENMIIDLEGR YHIDYKNRNY KKLKPQMFST 70 80 90 100 110 120 FFEHYEIVQA DSLINSDFIK NKTVKKNRDN SSNNNSNNNS NNNNNNNTLN NSTIITTTTT 130 140 150 160 170 180 TTTTSTPTTT IMITPPQQQQ QQRTSLDLTN RDISESSTPN EQQIRLAQEE TENQEIVESS 190 200 210 220 230 240 FLKSSPVPSP SSSVLKSFES DFQLNTDLTT ETFDDNSAEK KRQQQQQQQQ NEDSKQSSQQ 250 260 270 280 290 300 QTQKSKDKDE SAKIVNNKSS STTNIKPILA AAQTSRSTSI PAFNRNKTKE PTKQKIKKEH 310 320 330 340 350 360 STLRKNSLSS SNIITPNNTT NTNAKDGASY FNENSLMSVK SDIKIFSLDL SINRLENITN 370 380 390 400 410 420 DILSIMARFE IQELTLSTNF FQIIPDLQLV KSLTTVNLTR NKLSKLQTSV FIELPSLTSL 430 440 450 460 470 480 ILDRNFISSI PDDIDQIKNL KYLSIKHNAL EYLPNSLSNL SQLISLDLSQ NKLKTLPPNF 490 500 510 520 530 540 DDLINLRMVW LSYNQITSLP SMRKLVNLVT FDISSNKLLS LPKDFAYLVP SRIKQSYSDI 550 560 570 580 590 600 DIDEYDENIN TCNNINSNNN DSNNSNNNNN NNNDNNNNCN KNNILEMINS TELEGGGLGC 610 620 630 640 650 660 LKELNIRDNR ELISLPVEYK QVESLMTLVT SIPSEIIPGI FLGGLDSANN APILQTLGIT 670 680 690 700 710 720 HILLAIGDCE PFFPKTFKYY SIDDARDAPQ YDISQHFEQT NCFIESGRKS GGVLVHCRAG 730 740 750 760 770 780 ISRSSTLVIS YLMKYQRMTF KQAMDLVQSK RPQIQPNPGF KDQLLKYEAK LFCTNILNIS 790 800 810 820 830 840 SHNSNNKNNN SSNNRKSINN RKSNNIIITI NNSSNSNNNN STDNSNNSST STTPNLSSLS 850 SDSSSSASLS KLSISK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000023 Genomic DNA. Translation: EAL68395.1.
RefSeq	XP_642370.1. XM_637278.1.
3D structure databases
HSSP	HSSP built from PDB template 1M3G based on UniProtKB Q05923.
ProteinModelPortal	Q54Y32.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0238874; DDB0238874; DDB_G0278445.
GeneID	8621575.
KEGG	ddi:DDB_G0278445.
Organism-specific databases
dictyBase	DDB_G0278445. mpl3.
Phylogenomic databases
eggNOG	COG4886.
OMA	DSANNAP.
Family and domain databases
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
SMART	SM00195. DSPc. 1 hit. SM00369. LRR_TYP. 1 hit. [Graphical view]
PROSITE	PS51450. LRR. 9 hits. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPL3_DICDI

Accession

Primary (citable) accession number: Q54Y32

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)