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Protein

Probable ubiquitin-conjugating enzyme E2 C

Gene

ube2c

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_272936. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_279384. Senescence-Associated Secretory Phenotype (SASP).
REACT_281710. Regulation of APC/C activators between G1/S and early anaphase.
REACT_295029. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_312949. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_317678. APC/C:Cdc20 mediated degradation of Securin.
REACT_319046. Separation of Sister Chromatids.
REACT_322901. Phosphorylation of the APC/C.
REACT_332932. APC-Cdc20 mediated degradation of Nek2A.
REACT_338704. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_342159. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_344410. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_351052. Autodegradation of Cdh1 by Cdh1:APC/C.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 C (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein C
Ubiquitin-protein ligase C
Gene namesi
Name:ube2c
Synonyms:ubch10
ORF Names:DDB_G0278775
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278775. ube2c.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153Probable ubiquitin-conjugating enzyme E2 CPRO_0000328509Add
BLAST

Post-translational modificationi

Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Ubl conjugation

Interactioni

Subunit structurei

Component of the APC/C complex.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0304925.

Structurei

3D structure databases

ProteinModelPortaliQ54XS6.
SMRiQ54XS6. Positions 3-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiQ54XS6.
KOiK06688.
OMAiAELWDKD.
PhylomeDBiQ54XS6.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54XS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDHSVSKR LQSELMNLMM GPSPGISAFP DGDNIFNWIG TIQGAKDTVY
60 70 80 90 100
EQMEFKMSLK FPTDYPYKPP TVKFETQCFH PNVDNNGNIC LDILKDKWSP
110 120 130 140 150
VYNVRSLLIS IQSLLGEPNN ESPLNSYAAS LWSNQDEYKK VLDKRYQEAT

SRP
Length:153
Mass (Da):17,473
Last modified:May 24, 2005 - v1
Checksum:i4ACE8F6E07C6E6C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000024 Genomic DNA. Translation: EAL67989.1.
RefSeqiXP_641954.1. XM_636862.1.

Genome annotation databases

EnsemblProtistsiDDB0304925; DDB0304925; DDB_G0278775.
GeneIDi8621686.
KEGGiddi:DDB_G0278775.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000024 Genomic DNA. Translation: EAL67989.1.
RefSeqiXP_641954.1. XM_636862.1.

3D structure databases

ProteinModelPortaliQ54XS6.
SMRiQ54XS6. Positions 3-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0304925.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0304925; DDB0304925; DDB_G0278775.
GeneIDi8621686.
KEGGiddi:DDB_G0278775.

Organism-specific databases

dictyBaseiDDB_G0278775. ube2c.

Phylogenomic databases

eggNOGiCOG5078.
InParanoidiQ54XS6.
KOiK06688.
OMAiAELWDKD.
PhylomeDBiQ54XS6.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_272936. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_279384. Senescence-Associated Secretory Phenotype (SASP).
REACT_281710. Regulation of APC/C activators between G1/S and early anaphase.
REACT_295029. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_312949. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_317678. APC/C:Cdc20 mediated degradation of Securin.
REACT_319046. Separation of Sister Chromatids.
REACT_322901. Phosphorylation of the APC/C.
REACT_332932. APC-Cdc20 mediated degradation of Nek2A.
REACT_338704. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_342159. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_344410. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_351052. Autodegradation of Cdh1 by Cdh1:APC/C.

Miscellaneous databases

PROiQ54XS6.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiUBE2C_DICDI
AccessioniPrimary (citable) accession number: Q54XS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.