Reviewed, UniProtKB/Swiss-Prot Q54XS2 (ACON_DICDI)

Names and origin

Protein names

Recommended name:
    Probable aconitate hydratase, mitochondrial
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	aco2
Synonyms:	acnB
ORF Names:	DDB_G0278779

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	771 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 771		Probable aconitate hydratase, mitochondrial		PRO_0000328572
Sites
	Metal binding	372	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	435	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	438	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54XS2-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 152D8580014A1152	FASTA	771	83,542
	10 20 30 40 50 60 MNSLVKGISK VRSTRSFSTV SMSPLEPNKK LNYEGIDAKL KQFRLHHNKP LTLAEKIIYG 70 80 90 100 110 120 HLEDPSTKVE RGITYLKLHP DRVAMQDATA QMAVLQFMSA GLPETAVPTT IHCDHLIEAY 130 140 150 160 170 180 KGGEKDLEVA KDINKEVYDF LSTSAKKFGM GFWKPGSGII HQIVLENYAF PGGLMIGTDS 190 200 210 220 230 240 HTPNAGGLGM VAVGVGGADA VDVMAGIPWE LKAPKIIGVK LTGSLKGWSS PKDVILRVAD 250 260 270 280 290 300 ILTVKGGTGA IVEYFGSGVE SLSCTGMATI CNMGAEIGAT TSLFPFNKRM VDYLNSTGRS 310 320 330 340 350 360 NIANAANSFK HNLVADPNAH YDQLIELNLD TLEPYINGPF TPDLGHPLSK FAESVKTNNW 370 380 390 400 410 420 PAELKVGLIG SCTNSSYEDM SRSASVAQQA LDKGITAKAK FTITPGSEQI RATIERDGQM 430 440 450 460 470 480 KVLEKVGGVV LANACGPCIG QWKREDVPKG EKNSIITSYN RNFTGRNDSN VNTHAFVASP 490 500 510 520 530 540 EIVTALTIAG DITFNPMTDF LTDKDGNKFK LTPPTGDELP SRGFDAGENT YQPPSPNGQN 550 560 570 580 590 600 INVIVDSESS RLQLLQPFAP WDKKDLVDMQ VLIKVQGKCT TDHISMAGPW LKYRGHLDNI 610 620 630 640 650 660 SNNMLIGAIN SENGKANAVL NQFTGEIGPV PTVARDYKKR GVNWIVVGDE NYGEGSSREH 670 680 690 700 710 720 AALEPRHLGG KAILVKSFAR IHETNLKKQG ILPLTFANPS DYDKISGDDR ISIIGLKDLA 730 740 750 760 770 PGKQLTLIVK SAKQGSEFEI KANHTMNAGQ IEWFKAGSAL NYIKSEKAKK N

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000024 Genomic DNA. Translation: EAL67991.1.
RefSeq	XP_641958.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54XS2.
Genome annotation databases
GeneID	3393141.
GenomeReviews	Gene locus aco2 in contig CM000152_GR.
KEGG	ddi:DDB_0230168.
Organism-specific databases
dictyBase	DDB_G0278779. aco2.
Phylogenomic databases
OMA	PGKPLKC.
Enzyme and pathway databases
BRENDA	4.2.1.3. 424.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR006248. Aconitase_mito-like. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF5. Aconitase_mito. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01340. aconitase_mito. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_DICDI

Accession

Primary (citable) accession number: Q54XS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)