Reviewed, UniProtKB/Swiss-Prot Q54XS1 (PH4H_DICDI)

Names and origin

Protein names

Recommended name:
    Probable phenylalanine-4-hydroxylase
      Short name=PAH
    EC=1.14.16.1
Alternative name(s):
    Phe-4-monooxygenase

Gene names

Name:	pah
ORF Names:	DDB_G0278781

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-phenylalanine + tetrahydrobiopterin + O2 = L-tyrosine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Binds 1 Fe2+ ion By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 1/6.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Phenylalanine catabolism
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Probable phenylalanine-4-hydroxylase		PRO_0000328336
Regions
	Domain	23 – 89	67	ACT
Sites
	Metal binding	273	1	Iron By similarity
	Metal binding	278	1	Iron By similarity
	Metal binding	318	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54XS1-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: D02E03F28A873D1C	FASTA	441	50,415
	10 20 30 40 50 60 MESNTNSQGQ GIIPQSYHSS IFFSISKGSD KIGGLLEYLE IIKKHNINIT RIESRPSKTE 70 80 90 100 110 120 KKDYDFFLDL EYPTENNKEV EKVIKDLEEK GVKATTLQES SNQTYAPWFP RKISDLDLFA 130 140 150 160 170 180 NKVLEMGSDL TSDHPGASDP VYRERRREIA KIASTYKHGD EIPRIDYTEE EIKTWGVVYN 190 200 210 220 230 240 RLKELFPTNA CHQHAYIFPL LEQNCGYSPD NIPQLQDISN FLQECTGWRI RPVQGLLSAR 250 260 270 280 290 300 DFLNGLAFRV FHATQYIRHP SVPLYTPEPD CCHELLGHVP LLADPDFADF SQEIGLASIG 310 320 330 340 350 360 ASDEDIQLLS TCYWFTVEFG LCKEGDTIRA YGAGILSSTG EMEHFLTDKA KKLPFNPFDA 370 380 390 400 410 420 CNTEYPITTF QPLYYVAESF QKAKEQMRQF ADSFKKPFSI RYNPYTQSIE ILDNKDKLLN 430 440 ICNDIRNQSE ILADAISKLK A

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000024 Genomic DNA. Translation: EAL67992.1.
RefSeq	XP_641959.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3393142.
KEGG	ddi:DDB_0231664.
Organism-specific databases
dictyBase	DDB_G0278781. pah.
Phylogenomic databases
OMA	Q54XS1. NALAFRV.
Enzyme and pathway databases
BRENDA	1.14.16.1. 424.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PH4H_DICDI

Accession

Primary (citable) accession number: Q54XS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)