Q54XS1 (PH4H_DICDI) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 53.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Phenylalanine-4-hydroxylase Short name=PAH EC=1.14.16.1 EC=1.14.16.4 Alternative name(s): Phe-4-monooxygenase Tryptophan 5-hydroxylase Short name=TRH Tryptophan 5-monooxygenase | ||||
| Gene names |
| ||||
| Organism | Dictyostelium discoideum (Slime mold) | ||||
| Taxonomic identifier | 44689 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium |
Protein attributes
| Sequence length | 441 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Hydroxylates L-tryptophan to 5-hydroxy-L-tryptophan but does not hydroxylate L-tyrosine to L-DOPA. It uses D-threo-tetrahydrodictyopterin (DH4), also known as dictyoperin, as a cofactor. Ref.2 |
| Catalytic activity | L-phenylalanine + tetrahydrobiopterin + O2 = L-tyrosine + 4a-hydroxytetrahydrobiopterin. L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin. |
| Cofactor | Binds 1 Fe2+ ion By similarity. |
| Pathway | |
| Subunit structure | Homoetramer. Ref.2 |
| Miscellaneous | This enzyme uses tetrahydrodictyopterin (DH4), a D-threo isomer of biopterin, and not tetrahydrobiopterin (TH4) for it's catalytic activity. |
| Sequence similarities | Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain. |
| Biophysicochemical properties | Kinetic parameters: KM=620 µM for for L-Phe with BH4 as cofactor Ref.2 KM=90 µM for for L-Phe with DH4 as cofactor KM=49 µM for for BH4 KM=39 µM for for DH4 Vmax=660 nmol/min/mg enzyme with BH4 as cofactor (preincubated with L-Phe) Vmax=840 nmol/min/mg enzyme with DH4 as cofactor (preincubated with L-Phe) Vmax=1620 nmol/min/mg enzyme with BH4 as cofactor (preincubated with BH4) Vmax=1890 nmol/min/mg enzyme with DH4 as cofactor (preincubated with DH4) Temperature dependence: Optimum temperature is 40 degrees Celsius. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Phenylalanine catabolism |
| Ligand | Iron Metal-binding |
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: InterPro L-phenylalanine catabolic processInferred from direct assay Ref.2. Source: dictyBase protein tetramerizationInferred from direct assay Ref.2. Source: dictyBase tyrosine biosynthetic processInferred from direct assay Ref.2. Source: dictyBase |
| Molecular function | iron ion binding Inferred from electronic annotation. Source: InterPro phenylalanine 4-monooxygenase activityInferred from direct assay Ref.2. Source: dictyBase prephenate dehydratase activityInferred from electronic annotation. Source: InterPro tetrahydrobiopterin bindingInferred from direct assay Ref.2. Source: dictyBase tryptophan 5-monooxygenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. Kuspa A.Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4. |
| [2] | "The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum." Siltberg-Liberles J., Steen I.H., Svebak R.M., Martinez A. Gene 427:86-92(2008) [PubMed: 18835579] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AAFI02000024 Genomic DNA. Translation: EAL67992.1. |
| RefSeq | XP_641959.1. XM_636867.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1TOH based on UniProtKB P04177. |
| ProteinModelPortal | Q54XS1. |
| SMR | Q54XS1. Positions 107-440. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q54XS1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblProtists | DDB0231664; DDB0231664; DDB_G0278781. |
| GeneID | 8621691. |
| GenomeReviews | Gene locus pah in contig CM000152_GR. |
| KEGG | ddi:DDB_G0278781. |
Organism-specific databases | |
| dictyBase | DDB_G0278781. pah. |
Phylogenomic databases | |
| eggNOG | KOG3820. |
| GeneTree | EPrGT00050000005952. |
| HOGENOM | HBG484724. |
| OMA | PRAYENK. |
| PhylomeDB | Q54XS1. |
| ProtClustDB | CLSZ2729099. |
Family and domain databases | |
| InterPro | IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR018528. Preph_deHydtase_CS. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view] |
| Gene3D | G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit. |
| KO | K00500. |
| PANTHER | PTHR11473. Aaa_hydroxylase. 1 hit. |
| Pfam | PF00351. Biopterin_H. 1 hit. [Graphical view] |
| PIRSF | PIRSF000336. TH. 1 hit. |
| PRINTS | PR00372. FYWHYDRXLASE. |
| SUPFAM | SSF56534. Aaa_hydroxylase. 1 hit. |
| PROSITE | PS00367. BH4_AAA_HYDROXYL_1. 1 hit. PS51410. BH4_AAA_HYDROXYL_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PH4H_DICDI | ||||||||
| Accession | Primary (citable) accession number: Q54XS1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Dictyostelium discoideum Dictyostelium discoideum: entries, gene names and cross-references to dictyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with