Reviewed, UniProtKB/Swiss-Prot Q54XC1 (5NTC_DICDI)

Names and origin

Protein names

Recommended name:
    Cytosolic purine 5'-nucleotidase
    EC=3.1.3.5
Alternative name(s):
    5'-nucleotidase cytosolic II

Gene names

Name:	nt5c2
ORF Names:	DDB_G0279053

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the 5'(3')-deoxyribonucleotidase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phagocytic vesicle Inferred from direct assay. Source: dictyBase
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Cytosolic purine 5'-nucleotidase		PRO_0000356849
Regions
	Region	299 – 307	9	Substrate binding Potential
	Compositional bias	4 – 15	12	Poly-Asn
Sites
	Active site	122	1	Nucleophile By similarity
	Active site	124	1	Proton donor By similarity
	Metal binding	122	1	Magnesium By similarity
	Metal binding	124	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	444	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54XC1-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: ECC63D2313E3577F	FASTA	592	68,352
	10 20 30 40 50 60 MAENNNNNNN NNNNNVSTPP HQKPHLTTGL RTSSSGLLMD KRRQDEEKFS AEQVAMGKKS 70 80 90 100 110 120 PTKLFSAYSL NDLSNPPTDE ELNNYKDLPA SSLPPIHKRE KLRRVFVNRD IKLDRIEFFG 130 140 150 160 170 180 FDMDYTLAVY NSPDFEELAY DMVLDKLIDI GYPKSIRKLK YDPNFPTRGL FLDRELGNLL 190 200 210 220 230 240 KIDSFGNIII CVHGRTTLSK NRTAEFYPSM RVSSDEIARN RFYLLNTLFT LPEACLYADL 250 260 270 280 290 300 VDHLERESGL RLTEEVADEQ QQMNSPPLSS LGSESVRIDE SNHQPEGDLS FSNLFQDVRT 310 320 330 340 350 360 ACDLVHNDGS LKTRVLDDLP RYIRKTPDMP VFFDRLRQNK NKVFLLTNSE FYYTNKVMSY 370 380 390 400 410 420 MMNGYNPNYQ SWRDYFDVII VGADKPRFFS EGTTIREVDT ETGNLRITNV KDRFEQGKVY 430 440 450 460 470 480 HGGSLSLFQK LTGAKGSRVL YIGDHIFADI IKSKKTHGWR NLLVVPELQH ELEVMNQQKD 490 500 510 520 530 540 TTIHLMNLEF IRAEIYRGLD SESTTPPDIE VLHQHLKNTN DKLNFAYNKY FGSLFKNGSK 550 560 570 580 590 STFFSMQVQR YADLYTSDYL NLLNYPLFYH FCANSLPLPH ESSSFSSFDT SN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000026 Genomic DNA. Translation: EAL67948.1.
RefSeq	XP_641940.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3393029.
GenomeReviews	Gene locus nt5c2 in contig CM000152_GR.
KEGG	ddi:DDB_0230202.
Organism-specific databases
dictyBase	DDB_G0279053. nt5c2.
Phylogenomic databases
OMA	IGAKGKD.
Family and domain databases
InterPro	IPR008380. HAD-SF_hydro_IG_5-nucl. IPR016695. Pur_nucleotidase. [Graphical view]
PANTHER	PTHR12103. HAD-SF_hydro_IG_5-nucl. 1 hit.
Pfam	PF05761. 5_nucleotid. 1 hit. [Graphical view]
PIRSF	PIRSF017434. Purine_5'-nucleotidase. 1 hit.
TIGRFAMs	TIGR02244. HAD-IG-Ncltidse. 1 hit.
ProtoNet	Search...

Entry information

Entry name

5NTC_DICDI

Accession

Primary (citable) accession number: Q54XC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)