Reviewed, UniProtKB/Swiss-Prot Q54X95 (SYMC_DICDI)

Names and origin

Protein names

Recommended name:
    Probable methionyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metS
ORF Names:	DDB_G0279113

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	Probable methionyl-tRNA synthetase, cytoplasmic		PRO_0000328567
Regions
	Domain	573 – 680	108	tRNA-binding
	Motif	25 – 35	11	"HIGH" region By similarity
	Motif	346 – 350	5	"KMSKS" region By similarity
Sites
	Binding site	349	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54X95-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 058DFE76A1857801	FASTA	736	83,132
	10 20 30 40 50 60 MSKPSNTPPL PKDGERNILI TSALPYVNNV PHLGNIIGCV LSADVYARYC RLKNYNCIYI 70 80 90 100 110 120 CGTDEYGTAT ETKALSEGCT PKEICDKYHE IHKEIYEWFN ISFDKFGRTS TNSQTEIAQD 130 140 150 160 170 180 IFNKIKDNGY TLTQEIEQLY CEQTCKMFLA DRFVEGTCPH CKFEDARGDQ CDGCSKLLNP 190 200 210 220 230 240 TELINPRCKV CSKPPVIKST KHIFIDLPQL QQQVDQFVET NSKGGNWSEN SIAITNTWVK 250 260 270 280 290 300 GELKPRCITR DLKWGTPVPM EEFKDKVFYV WFDAPIGYIS ITAEYTNEWE KWWKNPENVK 310 320 330 340 350 360 LVQFMGKDNV PFHTVIFPAS LIGSKDNYTL LNNLSTTEFL NYETGKFSKS RNTGVFGDGA 370 380 390 400 410 420 KATGIPSEVW RFYLLNNRPE SSDSIFSWDD FNFKNNELLN NFGNLVNRVL KMLNTNAAFN 430 440 450 460 470 480 GVVPKIGELN EVDKKLVQEV DEHLVQYFQK LEEISLKEGL KIAMSISKLG NTYMQDNKPW 490 500 510 520 530 540 DLSGKDNERC GQVLAILINL IKLLSTLLEP YIPSLTDKVH QQLNVEPTKY STHFDINAIP 550 560 570 580 590 600 AGHEISKEIL PLVKKIEADD LKKWRTKFSG IGPEFPIDMK IATVLEVNDH PSAENLYVIK 610 620 630 640 650 660 LSLGGDQTKT AVSGIKANFE KSQLIGKKLA VVLNLKPSKF KGVLSEAMIL VADDGQATKE 670 680 690 700 710 720 SLSFLVPSNP QSIEAGSKIA GKGMSIKPKP TIDYQKEFLH YDLTCIDKII SYNKSQLFIN 730 QNEPLVSEKI GNGKVR

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000027 Genomic DNA. Translation: EAL67895.1.
RefSeq	XP_641872.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54X95.
Genome annotation databases
GeneID	3392965.
GenomeReviews	Gene locus metS in contig CM000152_GR.
KEGG	ddi:DDB_0231297.
Organism-specific databases
dictyBase	DDB_G0279113. metS.
Phylogenomic databases
OMA	CEAVPES.
Enzyme and pathway databases
BRENDA	6.1.1.10. 424.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMC_DICDI

Accession

Primary (citable) accession number: Q54X95

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)