Reviewed, UniProtKB/Swiss-Prot Q54X73 (ACOC_DICDI)

Names and origin

Protein names

Recommended name:
    Probable cytoplasmic aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	aco1
Synonyms:	acnA
ORF Names:	DDB_G0279159

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 894	894	Probable cytoplasmic aconitate hydratase		PRO_0000328573
Sites
	Metal binding	438	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	504	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	507	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54X73-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 8CE9854114B55202	FASTA	894	97,991
	10 20 30 40 50 60 MTTNNPFDKV KDVLKSQDQT YNFYNLSKLQ DPRIEKLPYS IRILLESAVR NCDNFEVHEK 70 80 90 100 110 120 DVENILNWEN TANKVEIPFK PARVLLQDFT GVPAVVDLAA MRDAMKRLGG DPAKINPLVP 130 140 150 160 170 180 VDLVIDHSVQ VDVSRTVDAL EQNQKIEFHR NHERFSFLKW GAQAFDGLFI APPGSGIVHQ 190 200 210 220 230 240 VNLEYIAREV MNGTGNLLYP DSVVGTDSHT TMINGLGVCG WGVGGIEAEA VMLGQPMSMV 250 260 270 280 290 300 LPEVVGYKFV GKLPDIATAT DLVLTVTNEL RKKGVVGKFV EFYGEGVSTL SVQDRATISN 310 320 330 340 350 360 MAPEYGATMG FFPADENTID YLASTGRSNT KIEYIKNYLS SQGLMCNYKS QSHPIFTTTM 370 380 390 400 410 420 ELDLSTVVPS LSGPKRPHDR ISLNSMKQDF NSCLSSPVGF KGFGLTADQI QKKATFTFKD 430 440 450 460 470 480 KQYTIGHGAV TIAAITSCTN TSNPSVMLGA GLLAKNAVEH GLEVAPYIKT SLSPGSGVVT 490 500 510 520 530 540 EYFSHSGLQE PLNKLGFDLT GYGCMTCIGN SGELAEPLAE AITKEDLVVA GVLSGNRNFE 550 560 570 580 590 600 GRIHPLLRAN YLASPPLVVA YALAGTVDID FETTPLGVSK KTGQPVFLRD IWPSKDLIQQ 610 620 630 640 650 660 TIKSSVLPDM YERVYSNVND GNKSWNELKV PTGLLYPWDE KSTYIHNPPF FKTMELTVSK 670 680 690 700 710 720 RPAITNAYCL LNLGDSITTD HISPAGNINR KSSAARYLES KGVKPEDFNT YGSRRGNDEI 730 740 750 760 770 780 MVRGTFANTR IVNKLAPAVG PQTTYVPTGE LMFISDAAEK YQSEGHQLIV LAGSDYGSGS 790 800 810 820 830 840 SRDWAAKGPY LQGIKCVIAI SFERIHRSNL VGMGIIPLQF QPGQNASTLG LTGKEQFNIE 850 860 870 880 890 LPTDKSLIKT GQTVKVTTNC GKSFETILRF DTPIEVEYWA NNGILSYVLR KLLH

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000028 Genomic DNA. Translation: EAL67861.1.
RefSeq	XP_641837.1.
3D structure databases
HSSP	HSSP built from PDB template 2B3Y based on UniProtKB P21399.
SMR	Q54X73. Positions 4-892.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54X73.
Genome annotation databases
GeneID	8621900.
KEGG	ddi:DDB_0229908.
Organism-specific databases
dictyBase	DDB_G0279159. aco1.
Phylogenomic databases
eggNOG	KOG0452.
HOGENOM	HBG289738.
OMA	YSKAQGM.
PhylomeDB	Q54X73.
Enzyme and pathway databases
BRENDA	4.2.1.3. 424.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACOC_DICDI

Accession

Primary (citable) accession number: Q54X73

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)