Q54X49 (METE_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
ORF Names:	DDB_G0279211

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.
Cofactor	Zinc By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 825	824	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase		PRO_0000342368
Sites
	Metal binding	708	1	Zinc By similarity
	Metal binding	710	1	Zinc By similarity
	Metal binding	793	1	Zinc By similarity
	Site	2	1	Not acetylated

Sequences

	Sequence		Length	Mass (Da)
	Q54X49 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0AD6B7B9F57F5E67	FASTA	825	94,518
	10 20 30 40 50 60 MVSSSNLGFP RMGENRELKK LVENYWQGKV EETQFQKELK EIRINHWKLQ KEAGIEIIPS 70 80 90 100 110 120 NDFSLYDQVL DHIHLFGAIP KRYTPVVESE IEDGSNGLRT YFAMGRGYQT SKAATQVSET 130 140 150 160 170 180 NTQGAFASKV EKSIDVGSME MKKWFDTNYH YIVPEFEDSQ QFKLTNYGGY SEPKPIHEFL 190 200 210 220 230 240 EAKSIDIETR PVVLGPISFL LLGKSSDSSK PYSSKFDSLV HLNALLGVYE ELFKQFEKIG 250 260 270 280 290 300 VKSVQIDEPV LCFDLFETEL IKNAYTTAFN VIHKAAPNIN ILIATYFGEI RENIDLITSL 310 320 330 340 350 360 PVNGIHIDTI RSSSSEVESV LTKIPTSWTI SIGIIDGRNV WKNDLTKSLV TLKNIIAKTG 370 380 390 400 410 420 SDRILLAPSC SLLHCPHSLT REIGRVESQV LDWLAFSLEK LKEISFLTYA LNKCTFTTEN 430 440 450 460 470 480 TITFPEDTCS KIKEYYQLNK ESNQKRRESK LIHNDIVKKR VTEITPSMLK RENPFPVRRE 490 500 510 520 530 540 AQRKRLTTLP ALFPTTTIGS FPQTKEVRLA RSNFKSKKIT PEAYDQFIRD EIRKCIKVQE 550 560 570 580 590 600 ECELDVLVHG EFERTDMVEY FGEYLAGFVF TQNGWVQSYG SRCVKPPIIY GDVNRPVPMT 610 620 630 640 650 660 LEYTTFAQTL TTKPMKGMLT GPVTILQWSF VRDDQPRSET CFQIGLAIRD EVTDLENKGI 670 680 690 700 710 720 ACIQIDEPAI REGLPLRLSD WNQYLKWAID SFLLSSTGVK DSTQIHSHMC YSDFNDIFES 730 740 750 760 770 780 IQRMDTDVLT IENSKSDLKL LKAFEKYGYT NEIGPGLYDI HSPRIPSVED MKDRVEQMLK 790 800 810 820 YLSTNLLWIN PDCGLKTREP ETTRLALINM VKVAKQFREI YANKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Veltman D.M., Insall R.H. Submitted (JAN-2010) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-11; 82-99; 113-129; 164-183; 312-323; 432-440; 461-471; 678-686; 724-735; 747-764 AND 805-812, CLEAVAGE OF INITIATOR METHIONINE, LACK OF N-TERMINAL ACETYLATION, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000030 Genomic DNA. Translation: EAL67754.1.
RefSeq	XP_641728.1. XM_636636.1.
3D structure databases
HSSP	HSSP built from PDB template 1U1J based on UniProtKB O50008.
ProteinModelPortal	Q54X49.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54X49.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0230069; DDB0230069; DDB_G0279211.
GeneID	8621924.
GenomeReviews	Gene locus metE in contig CM000152_GR.
KEGG	ddi:DDB_G0279211.
Organism-specific databases
dictyBase	DDB_G0279211.
Phylogenomic databases
eggNOG	KOG2263.
GeneTree	EPrGT00050000005903.
HOGENOM	HBG287495.
OMA	LWLSKSH.
PhylomeDB	Q54X49.
ProtClustDB	CLSZ2430700.
Family and domain databases
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_DICDI

Accession

Primary (citable) accession number: Q54X49

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)