Q54WU3 (AMPM1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase 1
Short name=MAP 1
Short name=MetAP 1
EC=3.4.11.18

Alternative name(s):
Peptidase M 1

Gene names

Name:	metap1
ORF Names:	DDB_G0279433

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Methionine aminopeptidase 1		PRO_0000328513
Sites
	Metal binding	202	1	Cobalt 1 By similarity
	Metal binding	213	1	Cobalt 1 By similarity
	Metal binding	213	1	Cobalt 2 By similarity
	Metal binding	276	1	Cobalt 2 By similarity
	Metal binding	309	1	Cobalt 2 By similarity
	Metal binding	340	1	Cobalt 1 By similarity
	Metal binding	340	1	Cobalt 2 By similarity
	Binding site	185	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54WU3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 141BCCF2CF09A2BD	FASTA	367	41,185
	10 20 30 40 50 60 MEGILCASPG CGKPAKLQCP TCVNLKLETP SHFCSQECFK TFWPLHKMYH QKGQPENLPS 70 80 90 100 110 120 QFRNYKFTGP LRPTNITPMR KAPEGIELPD YAIGSIPISE RVADRKNMAN IIHTPEEIEI 130 140 150 160 170 180 MRQLGKMSRE VLDIAGNAAK VGMTTEELDI IVHNAVIERG AYPSPLNYYK FPKSCCTSLN 190 200 210 220 230 240 EVICHGIPDE RPLRDGDILN VDVTLYWKGF HSDLNETYLI GNVDERGKNL VKCAYDCLEL 250 260 270 280 290 300 AVAMCKPGTL YRELGDAIQK HANKQGFSVV KNFCGHGIGR LFHCNPTVPH YSKNKAVGAM 310 320 330 340 350 360 KVGHVFTIEP MINEGTWQDE IWPDSWTAVT ADGKRSAQFE HTLVITETGC EVLTKRTNGS YIDRHFK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000031 Genomic DNA. Translation: EAL67655.1.
RefSeq	XP_641624.1. XM_636532.1.
3D structure databases
HSSP	HSSP built from PDB template 2B3H based on UniProtKB P53582.
ProteinModelPortal	Q54WU3.
SMR	Q54WU3. Positions 65-358.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54WU3.
Protein family/group databases
MEROPS	M24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0235405; DDB0235405; DDB_G0279433.
GeneID	8622030.
GenomeReviews	Gene locus metap1 in contig CM000152_GR.
KEGG	ddi:DDB_G0279433.
Organism-specific databases
dictyBase	DDB_G0279433. metap1.
Phylogenomic databases
eggNOG	KOG2738.
GeneTree	EPrGT00050000000956.
HOGENOM	HBG299384.
OMA	QEGMRKV.
PhylomeDB	Q54WU3.
ProtClustDB	CLSZ2429083.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KO	K01265.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM1_DICDI

Accession

Primary (citable) accession number: Q54WU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)