Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q54WU3

- MAP11_DICDI

UniProt

Q54WU3 - MAP11_DICDI

Protein

Methionine aminopeptidase 1

Gene

metap1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei185 – 1851SubstrateUniRule annotation
    Metal bindingi202 – 2021Divalent metal cation 1UniRule annotation
    Metal bindingi213 – 2131Divalent metal cation 1UniRule annotation
    Metal bindingi213 – 2131Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi276 – 2761Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei283 – 2831SubstrateUniRule annotation
    Metal bindingi309 – 3091Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi340 – 3401Divalent metal cation 1UniRule annotation
    Metal bindingi340 – 3401Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Protein family/group databases

    MEROPSiM24.017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 1UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 1UniRule annotation
    Short name:
    MetAP 1UniRule annotation
    Alternative name(s):
    Peptidase M 1UniRule annotation
    Gene namesi
    Name:metap1
    ORF Names:DDB_G0279433
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0279433. metap1.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytosolic ribosome Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Methionine aminopeptidase 1PRO_0000328513Add
    BLAST

    Interactioni

    Subunit structurei

    Associates with the 60S ribosomal subunit of the 80S translational complex.UniRule annotation

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0205763.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54WU3.
    SMRiQ54WU3. Positions 65-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni6 – 5045Zinc finger-like; important for proper ribosome associationUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0024.
    KOiK01265.
    OMAiMSACEAV.
    PhylomeDBiQ54WU3.

    Family and domain databases

    Gene3Di3.90.230.10. 1 hit.
    HAMAPiMF_01974. MetAP_1.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002467. Pept_M24A_MAP1.
    [Graphical view]
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 1 hit.
    TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
    PROSITEiPS00680. MAP_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54WU3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGILCASPG CGKPAKLQCP TCVNLKLETP SHFCSQECFK TFWPLHKMYH    50
    QKGQPENLPS QFRNYKFTGP LRPTNITPMR KAPEGIELPD YAIGSIPISE 100
    RVADRKNMAN IIHTPEEIEI MRQLGKMSRE VLDIAGNAAK VGMTTEELDI 150
    IVHNAVIERG AYPSPLNYYK FPKSCCTSLN EVICHGIPDE RPLRDGDILN 200
    VDVTLYWKGF HSDLNETYLI GNVDERGKNL VKCAYDCLEL AVAMCKPGTL 250
    YRELGDAIQK HANKQGFSVV KNFCGHGIGR LFHCNPTVPH YSKNKAVGAM 300
    KVGHVFTIEP MINEGTWQDE IWPDSWTAVT ADGKRSAQFE HTLVITETGC 350
    EVLTKRTNGS YIDRHFK 367
    Length:367
    Mass (Da):41,185
    Last modified:May 24, 2005 - v1
    Checksum:i141BCCF2CF09A2BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000031 Genomic DNA. Translation: EAL67655.1.
    RefSeqiXP_641624.1. XM_636532.1.

    Genome annotation databases

    EnsemblProtistsiDDB0235405; DDB0235405; DDB_G0279433.
    GeneIDi8622030.
    KEGGiddi:DDB_G0279433.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000031 Genomic DNA. Translation: EAL67655.1 .
    RefSeqi XP_641624.1. XM_636532.1.

    3D structure databases

    ProteinModelPortali Q54WU3.
    SMRi Q54WU3. Positions 65-358.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0205763.

    Protein family/group databases

    MEROPSi M24.017.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0235405 ; DDB0235405 ; DDB_G0279433 .
    GeneIDi 8622030.
    KEGGi ddi:DDB_G0279433.

    Organism-specific databases

    dictyBasei DDB_G0279433. metap1.

    Phylogenomic databases

    eggNOGi COG0024.
    KOi K01265.
    OMAi MSACEAV.
    PhylomeDBi Q54WU3.

    Miscellaneous databases

    PROi Q54WU3.

    Family and domain databases

    Gene3Di 3.90.230.10. 1 hit.
    HAMAPi MF_01974. MetAP_1.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002467. Pept_M24A_MAP1.
    [Graphical view ]
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 1 hit.
    TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
    PROSITEi PS00680. MAP_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiMAP11_DICDI
    AccessioniPrimary (citable) accession number: Q54WU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3