SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q54WU3

- MAP11_DICDI

UniProt

Q54WU3 - MAP11_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Methionine aminopeptidase 1

Gene
metap1, DDB_G0279433
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val) By similarity.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei185 – 1851Substrate By similarity
Metal bindingi202 – 2021Divalent metal cation 1 By similarity
Metal bindingi213 – 2131Divalent metal cation 1 By similarity
Metal bindingi213 – 2131Divalent metal cation 2; catalytic By similarity
Metal bindingi276 – 2761Divalent metal cation 2; catalytic; via tele nitrogen By similarity
Binding sitei283 – 2831Substrate By similarity
Metal bindingi309 – 3091Divalent metal cation 2; catalytic By similarity
Metal bindingi340 – 3401Divalent metal cation 1 By similarity
Metal bindingi340 – 3401Divalent metal cation 2; catalytic By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.017.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 1 (EC:3.4.11.18)
Short name:
MAP 1
Short name:
MetAP 1
Alternative name(s):
Peptidase M 1
Gene namesi
Name:metap1
ORF Names:DDB_G0279433
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0279433. metap1.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytosolic ribosome Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Methionine aminopeptidase 1UniRule annotationPRO_0000328513Add
BLAST

Interactioni

Subunit structurei

Associates with the 60S ribosomal subunit of the 80S translational complex By similarity.

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0205763.

Structurei

3D structure databases

ProteinModelPortaliQ54WU3.
SMRiQ54WU3. Positions 65-358.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 5045Zinc finger-like; important for proper ribosome association By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0024.
KOiK01265.
OMAiMSACEAV.
PhylomeDBiQ54WU3.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54WU3-1 [UniParc]FASTAAdd to Basket

« Hide

MEGILCASPG CGKPAKLQCP TCVNLKLETP SHFCSQECFK TFWPLHKMYH    50
QKGQPENLPS QFRNYKFTGP LRPTNITPMR KAPEGIELPD YAIGSIPISE 100
RVADRKNMAN IIHTPEEIEI MRQLGKMSRE VLDIAGNAAK VGMTTEELDI 150
IVHNAVIERG AYPSPLNYYK FPKSCCTSLN EVICHGIPDE RPLRDGDILN 200
VDVTLYWKGF HSDLNETYLI GNVDERGKNL VKCAYDCLEL AVAMCKPGTL 250
YRELGDAIQK HANKQGFSVV KNFCGHGIGR LFHCNPTVPH YSKNKAVGAM 300
KVGHVFTIEP MINEGTWQDE IWPDSWTAVT ADGKRSAQFE HTLVITETGC 350
EVLTKRTNGS YIDRHFK 367
Length:367
Mass (Da):41,185
Last modified:May 24, 2005 - v1
Checksum:i141BCCF2CF09A2BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000031 Genomic DNA. Translation: EAL67655.1.
RefSeqiXP_641624.1. XM_636532.1.

Genome annotation databases

EnsemblProtistsiDDB0235405; DDB0235405; DDB_G0279433.
GeneIDi8622030.
KEGGiddi:DDB_G0279433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000031 Genomic DNA. Translation: EAL67655.1 .
RefSeqi XP_641624.1. XM_636532.1.

3D structure databases

ProteinModelPortali Q54WU3.
SMRi Q54WU3. Positions 65-358.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0205763.

Protein family/group databases

MEROPSi M24.017.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0235405 ; DDB0235405 ; DDB_G0279433 .
GeneIDi 8622030.
KEGGi ddi:DDB_G0279433.

Organism-specific databases

dictyBasei DDB_G0279433. metap1.

Phylogenomic databases

eggNOGi COG0024.
KOi K01265.
OMAi MSACEAV.
PhylomeDBi Q54WU3.

Miscellaneous databases

PROi Q54WU3.

Family and domain databases

Gene3Di 3.90.230.10. 1 hit.
HAMAPi MF_01974. MetAP_1.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 1 hit.
TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
PROSITEi PS00680. MAP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMAP11_DICDI
AccessioniPrimary (citable) accession number: Q54WU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi