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Q54WU3

- MAP11_DICDI

UniProt

Q54WU3 - MAP11_DICDI

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Protein

Methionine aminopeptidase 1

Gene

metap1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei185 – 1851SubstrateUniRule annotation
Metal bindingi202 – 2021Divalent metal cation 1UniRule annotation
Metal bindingi213 – 2131Divalent metal cation 1UniRule annotation
Metal bindingi213 – 2131Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi276 – 2761Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
Binding sitei283 – 2831SubstrateUniRule annotation
Metal bindingi309 – 3091Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi340 – 3401Divalent metal cation 1UniRule annotation
Metal bindingi340 – 3401Divalent metal cation 2; catalyticUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.017.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 1UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 1UniRule annotation
Short name:
MetAP 1UniRule annotation
Alternative name(s):
Peptidase M 1UniRule annotation
Gene namesi
Name:metap1
ORF Names:DDB_G0279433
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0279433. metap1.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytosolic ribosome Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Methionine aminopeptidase 1PRO_0000328513Add
BLAST

Interactioni

Subunit structurei

Associates with the 60S ribosomal subunit of the 80S translational complex.UniRule annotation

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0205763.

Structurei

3D structure databases

ProteinModelPortaliQ54WU3.
SMRiQ54WU3. Positions 65-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 5045Zinc finger-like; important for proper ribosome associationUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0024.
InParanoidiQ54WU3.
KOiK01265.
OMAiMSACEAV.
PhylomeDBiQ54WU3.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54WU3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGILCASPG CGKPAKLQCP TCVNLKLETP SHFCSQECFK TFWPLHKMYH
60 70 80 90 100
QKGQPENLPS QFRNYKFTGP LRPTNITPMR KAPEGIELPD YAIGSIPISE
110 120 130 140 150
RVADRKNMAN IIHTPEEIEI MRQLGKMSRE VLDIAGNAAK VGMTTEELDI
160 170 180 190 200
IVHNAVIERG AYPSPLNYYK FPKSCCTSLN EVICHGIPDE RPLRDGDILN
210 220 230 240 250
VDVTLYWKGF HSDLNETYLI GNVDERGKNL VKCAYDCLEL AVAMCKPGTL
260 270 280 290 300
YRELGDAIQK HANKQGFSVV KNFCGHGIGR LFHCNPTVPH YSKNKAVGAM
310 320 330 340 350
KVGHVFTIEP MINEGTWQDE IWPDSWTAVT ADGKRSAQFE HTLVITETGC
360
EVLTKRTNGS YIDRHFK
Length:367
Mass (Da):41,185
Last modified:May 24, 2005 - v1
Checksum:i141BCCF2CF09A2BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000031 Genomic DNA. Translation: EAL67655.1.
RefSeqiXP_641624.1. XM_636532.1.

Genome annotation databases

EnsemblProtistsiDDB0235405; DDB0235405; DDB_G0279433.
GeneIDi8622030.
KEGGiddi:DDB_G0279433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000031 Genomic DNA. Translation: EAL67655.1 .
RefSeqi XP_641624.1. XM_636532.1.

3D structure databases

ProteinModelPortali Q54WU3.
SMRi Q54WU3. Positions 65-358.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0205763.

Protein family/group databases

MEROPSi M24.017.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0235405 ; DDB0235405 ; DDB_G0279433 .
GeneIDi 8622030.
KEGGi ddi:DDB_G0279433.

Organism-specific databases

dictyBasei DDB_G0279433. metap1.

Phylogenomic databases

eggNOGi COG0024.
InParanoidi Q54WU3.
KOi K01265.
OMAi MSACEAV.
PhylomeDBi Q54WU3.

Miscellaneous databases

PROi Q54WU3.

Family and domain databases

Gene3Di 3.90.230.10. 1 hit.
HAMAPi MF_01974. MetAP_1.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 1 hit.
TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
PROSITEi PS00680. MAP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiMAP11_DICDI
AccessioniPrimary (citable) accession number: Q54WU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3