Q54WS9 (Y9461_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase DDB_G0279461
EC=3.1.3.16

Gene names

ORF Names:

DDB_G0279461

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	In the C-terminal section; belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Domain	Coiled coil
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1006	1006	Probable protein phosphatase DDB_G0279461		PRO_0000369245
Regions
	Domain	639 – 1006	368	PP2C-like
	Coiled coil	65 – 122	58	Potential
	Coiled coil	450 – 516	67	Potential
	Compositional bias	223 – 234	12	Poly-Asn
	Compositional bias	244 – 256	13	Poly-Asn
	Compositional bias	291 – 296	6	Poly-Gln
	Compositional bias	316 – 352	37	Poly-Asn
	Compositional bias	372 – 381	10	Poly-Asn
	Compositional bias	554 – 560	7	Poly-Asn
	Compositional bias	613 – 621	9	Poly-Asn
	Compositional bias	622 – 639	18	Poly-Thr
Sites
	Metal binding	784	1	Manganese 1 By similarity
	Metal binding	784	1	Manganese 2 By similarity
	Metal binding	785	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	956	1	Manganese 2 By similarity
	Metal binding	996	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54WS9 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: 173B5733030E9B62	FASTA	1,006	111,161
	10 20 30 40 50 60 MMVPSLSTSI SSPALFKNRE GGEEGNDGGL EQLQNQVNDL GDLNFNEEGD YNNDQQPTNE 70 80 90 100 110 120 EEGTADNELE SLMSLVNDNN NNNNNTSGID DDNNNDIDDN NNNNNNNNNN NNNNNNNKEG 130 140 150 160 170 180 LNDLFISSIN VSTLLNDLDQ LSDTHSHASV SNQSSNGSVR RGYDIKTQRS VGTKGQGGSS 190 200 210 220 230 240 GSSPRSNSLR VYRTFPYRGD HHFSWLNNIR DVDSSEDPNS CHNSNNNNKN NKNNQPSSTH 250 260 270 280 290 300 DQTNINNNNN NNCNDNEKPI KPNSTNGIRH SRKYKGLQQL DFLGSNIILP QQQQPQPIED 310 320 330 340 350 360 NQQVVPQPFQ VDAESNYNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNDNE NNQHPINPVN 370 380 390 400 410 420 TQNTQQNVTH SNNNNNNNNN NTAPPNQWSE LIESLGYGSQ SDEQKTFGST LDDLNNANSC 430 440 450 460 470 480 SDLKDLIVDG ISINNETSPS QSQEITDFSK IDNLNKNINI NNNNNTDSQQ PLPSIDVNFS 490 500 510 520 530 540 HNNNNNNNDN DNNNNNNNNN NNNNNNNNNN NIQDIQIPYH QNDQDYNIQE GNDINNDNYE 550 560 570 580 590 600 IRVSNNDDDN DSSNNNNNNN SKFQENLNLL NTGMGGRICK TDQKKQLSRT ITFADPSSIL 610 620 630 640 650 660 SFFGSIPTDE DQNNNNNKNK NTTTTTTTTN TTTTTTTTTS ASAAAQNKLD LLSSLSAGQS 670 680 690 700 710 720 SGGVGGGNNV LNSSSTPAIK VNHQHKVTEQ NRTSSKISLS TIFPKLPPFT NQQSPPTLSP 730 740 750 760 770 780 SKYYYPLLQP EPTTLIRGFS SAADINKRGL KRAKKPMEME DVYLTQYPLG DDQDSQIALF 790 800 810 820 830 840 AIFDGHSGKG CAVAAKEIFP NILLKYIKST KNENGGKPIY DMRGVFLNAF KEVDAQLSKF 850 860 870 880 890 900 EYEGATATVC LVWRAGHQRF VQSANVGDST AFLSYGNETL FLSKDHRATD PEEIQRIKND 910 920 930 940 950 960 GITLTEGQTR INGLMVSRAL GDHFIKHLNC GLSGEPYVSP PISITPFHSH LIVASDGLWD 970 980 990 1000 VISGNRAMEI VKVQQTEEKM SNSLLQCAIG SIKAKDNISI IVVTLQ

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000031 Genomic DNA. Translation: EAL67669.2.
RefSeq	XP_641638.2. XM_636546.2.
3D structure databases
ProteinModelPortal	Q54WS9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0304651; DDB0304651; DDB_G0279461.
GeneID	8622044.
KEGG	ddi:DDB_G0279461.
Organism-specific databases
dictyBase	DDB_G0279461.
Phylogenomic databases
eggNOG	COG0631.
InParanoid	Q54WS9.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y9461_DICDI

Accession

Primary (citable) accession number: Q54WS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 14, 2009
Last modified:	March 6, 2013
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)