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Q54WD9 (SYYM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable tyrosine--tRNA ligase, mitochondrial
EC=6.1.1.1
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS
Gene names
Name:mtyrS
ORF Names:DDB_G0279715
OrganismDictyostelium discoideum (Slime mold)
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtyrosyl-tRNA aminoacylation

Inferred from sequence or structural similarity. Source: dictyBase

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

tyrosine-tRNA ligase activity

Inferred from sequence or structural similarity. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 482Probable tyrosine--tRNA ligase, mitochondrialPRO_0000331420

Regions

Domain416 – 48065S4 RNA-binding
Motif90 – 9910"HIGH" region By similarity
Motif279 – 2835"KMSKS" region By similarity
Compositional bias66 – 8015Asn-rich

Sites

Binding site2821ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54WD9 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: DF332E96B18755AA

FASTA48254,283
        10         20         30         40         50         60 
MIRNFTKGLL INNLNINKFG STKIRPFCST ITKNDKNNVI NILKKRGFIH QMTASDIEMI 

        70         80         90        100        110        120 
KMTSTNNNNN NDNNNNNNNN KVSLYAGFDP TADSLHIGNL LTLMVMLHFK RHGHNPIALM 

       130        140        150        160        170        180 
GGATALIGDP SGKSSDRVML TEEFIEGNLK HIRENITAVL GEDTLVVNNI EWNKTMDVIS 

       190        200        210        220        230        240 
FLREVGSYFR VGSMIKKDFI QNRLSDSNEN GISFTEFCYS LFQANDFAHL FLKHGCSIQI 

       250        260        270        280        290        300 
GGSDQWGNIT AGCDLIRKKL KGNAQGITIP LLTNSAGKKL GKSEGNSIWL SPHRTSPYKF 

       310        320        330        340        350        360 
YQYWIQVSDE DVERLLKLFT LIPLEEISIL VQKHNENPHL RLGQKTIAEH VTRLIHGQKG 

       370        380        390        400        410        420 
VEEALNTTEL LFGESNKLLT PNASTTKIGD FDIGYLLSRV NFIELERSKF VDNQTEPILN 

       430        440        450        460        470        480 
LFAQISDASK SKVKQLLQSK SIYLNNIPVA SNSQFIQSSD LIANDFIYLK SGKKIYYLIK 


FI

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFI02000032 Genomic DNA. Translation: EAL67632.1.
RefSeqXP_641614.1. XM_636522.1.

3D structure databases

HSSPHSSP built from PDB template 2TS1 based on UniProtKB P00952.
ProteinModelPortalQ54WD9.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ54WD9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231247; DDB0231247; DDB_G0279715.
GeneID8622192.
GenomeReviewsGene locus mtyrS in contig CM000152_GR.
KEGGddi:DDB_G0279715.

Organism-specific databases

dictyBaseDDB_G0279715. mtyrS.

Phylogenomic databases

eggNOGKOG2623.
GeneTreeEPrGT00050000001351.
HOGENOMHBG288125.
OMAHIRENIT.
PhylomeDBQ54WD9.
ProtClustDBCLSZ2497224.

Family and domain databases

InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-synth.
IPR024088. Tyr-tRNA-synth_bac-type.
IPR024107. Tyr-tRNA-synth_bac_1.
[Graphical view]
Gene3DG3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01866.
PANTHERPTHR11766. Tyr_tRNA-synt_1b. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01040. TRNASYNTHTYR.
TIGRFAMsTIGR00234. TyrS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYYM_DICDI
AccessionPrimary (citable) accession number: Q54WD9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

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