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Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

trmt5

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei204 – 2041S-adenosyl-L-methionineUniRule annotation
Binding sitei292 – 2921S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5 homologUniRule annotation
Gene namesi
Name:trmt5
ORF Names:DDB_G0279739
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0279739. trmt5.

Subcellular locationi

  • Mitochondrion matrix UniRule annotation
  • Nucleus UniRule annotation
  • Cytoplasm UniRule annotation

  • Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460tRNA (guanine(37)-N1)-methyltransferasePRO_0000414133Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi44689.DDB0237582.

Structurei

3D structure databases

ProteinModelPortaliQ54WD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 2442S-adenosyl-L-methionine bindingUniRule annotation
Regioni271 – 2722S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2520.
InParanoidiQ54WD6.
KOiK15429.
OMAiPVAKKNC.
PhylomeDBiQ54WD6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03152. TRM5.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR025792. tRNA_Gua_MeTrfase_euk.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54WD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLNKEVFN QTKKVLGLIV SNKLVNGFSK QFKSYMFQSP KFKPIVSTDN
60 70 80 90 100
DDKKMIYLCD TLKKETDIPD QLKTFIKEND IKVIEKDISL NYNNFSYEQV
110 120 130 140 150
LKTLLPKDVG IPFSFERIGH IIHVNLKDEQ LPFKYIIGQA ILDKNIQVKT
160 170 180 190 200
VLNKVGEIDT VFRTFKIEIL AGEPDLVAEI KENECIFRFN FEEVYWNSRL
210 220 230 240 250
QYEHMELVNT FKKEDIICDM FAGVGPFALP AAKIKKCKVY ANDLNPSSVK
260 270 280 290 300
YMKENAKTNR LESKVEISNL DARDFVKSLV EKSIPFTHVV MNLPSTSIEF
310 320 330 340 350
LDVFRDIFLN STIPPPIPPP IINCYTFTKL DESSDLIKDT IKNVENVIGA
360 370 380 390 400
KVPSDYVCYE VRDVAPKKSM MRITFRMPTI LPYVGSLTTA STTTTPTTSN
410 420 430 440 450
TNTSTTTSTT STSTTTTEST NTNNSANNVE DKNNKKRNST EDSNETNETD
460
SIDTNKKLKN
Length:460
Mass (Da):52,313
Last modified:May 24, 2005 - v1
Checksum:i074060D23690D668
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000032 Genomic DNA. Translation: EAL67549.1.
RefSeqiXP_641522.1. XM_636430.1.

Genome annotation databases

EnsemblProtistsiDDB0237582; DDB0237582; DDB_G0279739.
GeneIDi8622195.
KEGGiddi:DDB_G0279739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000032 Genomic DNA. Translation: EAL67549.1.
RefSeqiXP_641522.1. XM_636430.1.

3D structure databases

ProteinModelPortaliQ54WD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0237582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0237582; DDB0237582; DDB_G0279739.
GeneIDi8622195.
KEGGiddi:DDB_G0279739.

Organism-specific databases

dictyBaseiDDB_G0279739. trmt5.

Phylogenomic databases

eggNOGiCOG2520.
InParanoidiQ54WD6.
KOiK15429.
OMAiPVAKKNC.
PhylomeDBiQ54WD6.

Miscellaneous databases

PROiQ54WD6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03152. TRM5.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR025792. tRNA_Gua_MeTrfase_euk.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiTRM5_DICDI
AccessioniPrimary (citable) accession number: Q54WD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.