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Q54WD6 (TRM5_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine(37)-N1)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5 homolog
Gene names
Name:trmt5
ORF Names:DDB_G0279739
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding By similarity. HAMAP-Rule MF_03152

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_03152

Subunit structure

Monomer By similarity. HAMAP-Rule MF_03152

Subcellular location

Mitochondrion matrix By similarity. Nucleus By similarity. Cytoplasm By similarity. Note: Predominantly in the mitochondria and in the nucleus By similarity. HAMAP-Rule MF_03152

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
Mitochondrion
Nucleus
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460tRNA (guanine(37)-N1)-methyltransferase HAMAP-Rule MF_03152
PRO_0000414133

Regions

Region243 – 2442S-adenosyl-L-methionine binding By similarity
Region271 – 2722S-adenosyl-L-methionine binding By similarity

Sites

Binding site2041S-adenosyl-L-methionine By similarity
Binding site2921S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54WD6 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 074060D23690D668

FASTA46052,313
        10         20         30         40         50         60 
MNVLNKEVFN QTKKVLGLIV SNKLVNGFSK QFKSYMFQSP KFKPIVSTDN DDKKMIYLCD 

        70         80         90        100        110        120 
TLKKETDIPD QLKTFIKEND IKVIEKDISL NYNNFSYEQV LKTLLPKDVG IPFSFERIGH 

       130        140        150        160        170        180 
IIHVNLKDEQ LPFKYIIGQA ILDKNIQVKT VLNKVGEIDT VFRTFKIEIL AGEPDLVAEI 

       190        200        210        220        230        240 
KENECIFRFN FEEVYWNSRL QYEHMELVNT FKKEDIICDM FAGVGPFALP AAKIKKCKVY 

       250        260        270        280        290        300 
ANDLNPSSVK YMKENAKTNR LESKVEISNL DARDFVKSLV EKSIPFTHVV MNLPSTSIEF 

       310        320        330        340        350        360 
LDVFRDIFLN STIPPPIPPP IINCYTFTKL DESSDLIKDT IKNVENVIGA KVPSDYVCYE 

       370        380        390        400        410        420 
VRDVAPKKSM MRITFRMPTI LPYVGSLTTA STTTTPTTSN TNTSTTTSTT STSTTTTEST 

       430        440        450        460 
NTNNSANNVE DKNNKKRNST EDSNETNETD SIDTNKKLKN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000032 Genomic DNA. Translation: EAL67549.1.

3D structure databases

ProteinModelPortalQ54WD6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0206004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0237582; DDB0237582; DDB_G0279739.
KEGGddi:DDB_G0279739.

Organism-specific databases

dictyBaseDDB_G0279739. trmt5.

Phylogenomic databases

eggNOGCOG2520.
InParanoidQ54WD6.
KOK15429.
OMAENGMETE.
PhylomeDBQ54WD6.
ProtClustDBCLSZ2497221.

Family and domain databases

HAMAPMF_03152. TRM5.
InterProIPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view]
PfamPF02475. Met_10. 1 hit.
[Graphical view]
PROSITEPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54WD6.

Entry information

Entry nameTRM5_DICDI
AccessionPrimary (citable) accession number: Q54WD6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase