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Q54WD6

- TRM5_DICDI

UniProt

Q54WD6 - TRM5_DICDI

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Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

trmt5

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei204 – 2041S-adenosyl-L-methionineUniRule annotation
Binding sitei292 – 2921S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5 homologUniRule annotation
Gene namesi
Name:trmt5
ORF Names:DDB_G0279739
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0279739. trmt5.

Subcellular locationi

Mitochondrion matrix UniRule annotation. Nucleus UniRule annotation. Cytoplasm UniRule annotation
Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-HAMAP
  2. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460tRNA (guanine(37)-N1)-methyltransferasePRO_0000414133Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0206004.

Structurei

3D structure databases

ProteinModelPortaliQ54WD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 2442S-adenosyl-L-methionine bindingUniRule annotation
Regioni271 – 2722S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2520.
InParanoidiQ54WD6.
KOiK15429.
OMAiCFIRASE.
PhylomeDBiQ54WD6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03152. TRM5.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54WD6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNVLNKEVFN QTKKVLGLIV SNKLVNGFSK QFKSYMFQSP KFKPIVSTDN
60 70 80 90 100
DDKKMIYLCD TLKKETDIPD QLKTFIKEND IKVIEKDISL NYNNFSYEQV
110 120 130 140 150
LKTLLPKDVG IPFSFERIGH IIHVNLKDEQ LPFKYIIGQA ILDKNIQVKT
160 170 180 190 200
VLNKVGEIDT VFRTFKIEIL AGEPDLVAEI KENECIFRFN FEEVYWNSRL
210 220 230 240 250
QYEHMELVNT FKKEDIICDM FAGVGPFALP AAKIKKCKVY ANDLNPSSVK
260 270 280 290 300
YMKENAKTNR LESKVEISNL DARDFVKSLV EKSIPFTHVV MNLPSTSIEF
310 320 330 340 350
LDVFRDIFLN STIPPPIPPP IINCYTFTKL DESSDLIKDT IKNVENVIGA
360 370 380 390 400
KVPSDYVCYE VRDVAPKKSM MRITFRMPTI LPYVGSLTTA STTTTPTTSN
410 420 430 440 450
TNTSTTTSTT STSTTTTEST NTNNSANNVE DKNNKKRNST EDSNETNETD
460
SIDTNKKLKN
Length:460
Mass (Da):52,313
Last modified:May 24, 2005 - v1
Checksum:i074060D23690D668
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000032 Genomic DNA. Translation: EAL67549.1.
RefSeqiXP_641522.1. XM_636430.1.

Genome annotation databases

EnsemblProtistsiDDB0237582; DDB0237582; DDB_G0279739.
GeneIDi8622195.
KEGGiddi:DDB_G0279739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000032 Genomic DNA. Translation: EAL67549.1 .
RefSeqi XP_641522.1. XM_636430.1.

3D structure databases

ProteinModelPortali Q54WD6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0206004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0237582 ; DDB0237582 ; DDB_G0279739 .
GeneIDi 8622195.
KEGGi ddi:DDB_G0279739.

Organism-specific databases

dictyBasei DDB_G0279739. trmt5.

Phylogenomic databases

eggNOGi COG2520.
InParanoidi Q54WD6.
KOi K15429.
OMAi CFIRASE.
PhylomeDBi Q54WD6.

Miscellaneous databases

PROi Q54WD6.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03152. TRM5.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view ]
Pfami PF02475. Met_10. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiTRM5_DICDI
AccessioniPrimary (citable) accession number: Q54WD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3