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Q54WD6

- TRM5_DICDI

UniProt

Q54WD6 - TRM5_DICDI

Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

trmt5

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei204 – 2041S-adenosyl-L-methionineUniRule annotation
    Binding sitei292 – 2921S-adenosyl-L-methionineUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
    Alternative name(s):
    M1G-methyltransferaseUniRule annotation
    tRNA [GM37] methyltransferaseUniRule annotation
    tRNA methyltransferase 5 homologUniRule annotation
    Gene namesi
    Name:trmt5
    ORF Names:DDB_G0279739
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0279739. trmt5.

    Subcellular locationi

    Mitochondrion matrix UniRule annotation. Nucleus UniRule annotation. Cytoplasm UniRule annotation
    Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460tRNA (guanine(37)-N1)-methyltransferasePRO_0000414133Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0206004.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54WD6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni243 – 2442S-adenosyl-L-methionine bindingUniRule annotation
    Regioni271 – 2722S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2520.
    InParanoidiQ54WD6.
    KOiK15429.
    OMAiCFIRASE.
    PhylomeDBiQ54WD6.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03152. TRM5.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025792. tRNA_Gua_MeTrfase_euk.
    IPR003402. tRNA_Trfase_Trm5/Tyw2.
    [Graphical view]
    PfamiPF02475. Met_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54WD6-1 [UniParc]FASTAAdd to Basket

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    MNVLNKEVFN QTKKVLGLIV SNKLVNGFSK QFKSYMFQSP KFKPIVSTDN    50
    DDKKMIYLCD TLKKETDIPD QLKTFIKEND IKVIEKDISL NYNNFSYEQV 100
    LKTLLPKDVG IPFSFERIGH IIHVNLKDEQ LPFKYIIGQA ILDKNIQVKT 150
    VLNKVGEIDT VFRTFKIEIL AGEPDLVAEI KENECIFRFN FEEVYWNSRL 200
    QYEHMELVNT FKKEDIICDM FAGVGPFALP AAKIKKCKVY ANDLNPSSVK 250
    YMKENAKTNR LESKVEISNL DARDFVKSLV EKSIPFTHVV MNLPSTSIEF 300
    LDVFRDIFLN STIPPPIPPP IINCYTFTKL DESSDLIKDT IKNVENVIGA 350
    KVPSDYVCYE VRDVAPKKSM MRITFRMPTI LPYVGSLTTA STTTTPTTSN 400
    TNTSTTTSTT STSTTTTEST NTNNSANNVE DKNNKKRNST EDSNETNETD 450
    SIDTNKKLKN 460
    Length:460
    Mass (Da):52,313
    Last modified:May 24, 2005 - v1
    Checksum:i074060D23690D668
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000032 Genomic DNA. Translation: EAL67549.1.
    RefSeqiXP_641522.1. XM_636430.1.

    Genome annotation databases

    EnsemblProtistsiDDB0237582; DDB0237582; DDB_G0279739.
    GeneIDi8622195.
    KEGGiddi:DDB_G0279739.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000032 Genomic DNA. Translation: EAL67549.1 .
    RefSeqi XP_641522.1. XM_636430.1.

    3D structure databases

    ProteinModelPortali Q54WD6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0206004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0237582 ; DDB0237582 ; DDB_G0279739 .
    GeneIDi 8622195.
    KEGGi ddi:DDB_G0279739.

    Organism-specific databases

    dictyBasei DDB_G0279739. trmt5.

    Phylogenomic databases

    eggNOGi COG2520.
    InParanoidi Q54WD6.
    KOi K15429.
    OMAi CFIRASE.
    PhylomeDBi Q54WD6.

    Miscellaneous databases

    PROi Q54WD6.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03152. TRM5.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025792. tRNA_Gua_MeTrfase_euk.
    IPR003402. tRNA_Trfase_Trm5/Tyw2.
    [Graphical view ]
    Pfami PF02475. Met_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51684. SAM_MT_TRM5_TYW2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiTRM5_DICDI
    AccessioniPrimary (citable) accession number: Q54WD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 14, 2011
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3