Q54W36 (DHKH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hybrid signal transduction histidine kinase H
EC=2.7.13.3

Gene names

Name:	dhkH
ORF Names:	DDB_G0279913

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a receptor histidine kinase for a signal transduction pathway. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Post-translational modification	Activation probably requires transfer of a phosphate group between a histidine in the kinase core (transmitter) domain and an aspartate of the receiver domain By similarity.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAS (PER-ARNT-SIM) domain. Contains 1 response regulatory domain.
Sequence caution	The sequence AAK54089.2 differs from that shown. Reason: Frameshift at position 243.

Ontologies

Keywords
Biological process	Two-component regulatory system
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transducer Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1378	1378	Hybrid signal transduction histidine kinase H		PRO_0000328338
Regions
	Domain	243 – 314	72	PAS
	Domain	498 – 805	308	Histidine kinase
	Domain	1244 – 1364	121	Response regulatory
	Coiled coil	212 – 242	31	Potential
	Compositional bias	298 – 321	24	Poly-Asn
	Compositional bias	415 – 419	5	Poly-Asn
	Compositional bias	444 – 448	5	Poly-Asn
	Compositional bias	661 – 682	22	Poly-Asn
	Compositional bias	697 – 713	17	Poly-His
	Compositional bias	814 – 822	9	Poly-Gln
	Compositional bias	849 – 859	11	Poly-Asn
	Compositional bias	906 – 918	13	Poly-Asn
	Compositional bias	919 – 932	14	Poly-Thr
	Compositional bias	998 – 1010	13	Poly-Gln
	Compositional bias	1103 – 1115	13	Poly-Asn
	Compositional bias	1150 – 1189	40	Poly-Asn
Amino acid modifications
	Modified residue	501	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	1297	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	685	1	N → T in AAK54089. Ref.2
	Sequence conflict	699 – 700	2	Missing in AAK54089. Ref.2
	Sequence conflict	1339	1	L → F in AAK54089. Ref.2
	Sequence conflict	1367	1	R → G in AAK54089. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q54W36 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: EECF8D05DA08DC79	FASTA	1,378	156,521
	10 20 30 40 50 60 MNIGDDKSEV DVIIDSSSIT LNNNESSNNN TNIGDVLKFE SGLYNSFECE LINQVFNSVP 70 80 90 100 110 120 IMMGVCDLYD NSNSDFQIQD SQNYDFKFVI SNRCSYDNLH MFLDKGKGLD GHYSAKELHL 130 140 150 160 170 180 PAYFITLWIE NMLRSLRKKK SVKFMYPRYV DNGETPSSDD FYSQKIVWKK STMCFMGQVL 190 200 210 220 230 240 VPLDDINSNS NNNSNQKQTA CRFFFTSEEV TKEKFKKEEL INDFKSRLET LENKIDQRVD 250 260 270 280 290 300 ERIETRFKYV LESIPQMVWV TDNHGKIEFV NRQWKDYLGI DHSGQYLNWG SISYQQHNNN 310 320 330 340 350 360 NNNNNNNNNN NNNNNNNNSN NKSPIINSPN TTSPTNTQID FDSQWHYSLK EMKRFEMEIL 370 380 390 400 410 420 LQSMSGEYRW FLVRAEPYIE PNLGHPSSPL PSIISACVDN CDIMTDDNSS GILINNNNNI 430 440 450 460 470 480 QQPYLNNDNI SGDNVNNTPT CIDNNNNNID GSNNSVNSTG TEQLDIKWIG TCTDVNDQKT 490 500 510 520 530 540 AQDRIENAEK SKALFLQTMS HEMRTPLAGI MGINSWLSTS SPQLTSEQLD GCHTIDMCAE 550 560 570 580 590 600 ALLVLINNIL DLSKLEENKI ILEETEFYPT KIVEDSVDIL SSQAEQKKLD IIFQLKYNCL 610 620 630 640 650 660 SKVVGDFYRI RQVLTNLISN SVKFTPANGQ IIVGCEIYHE TTPSTRKRSS LDSIEITIPC 670 680 690 700 710 720 NHNNSNNSNN NHNHNNNNNN NNHLNCSGSF NNNGFNHGHH HHHHHHHHHH HHHDKHCDQK 730 740 750 760 770 780 IIVPGKYGKL LFWVIDNGIG IPEEGREKLF QTFSQYDAST TRKYGGSGLG LAISKRLTQL 790 800 810 820 830 840 LGGDIWFESQ KGKGSSFHFL VEVFFPDYPT IYNQQLQQQQ QQPNLPHQFN IGTSAPQPID 850 860 870 880 890 900 SILTSYSSNN SNNNINNNNI TTSVISQSPI VDKSNLCTYI FLLSTNQVLV NSLSQWINEW 910 920 930 940 950 960 IGNATNNNNS NNDNNNNNTT STTTTTTTTT ATINTNINQQ VKILYDIDSI EQISNSAIQS 970 980 990 1000 1010 1020 GKRLDFLFLI EDTFWNNYSD KISNSQEIIE SLINQNYQQQ QQQQQEQRQQ HNIKNVILSF 1030 1040 1050 1060 1070 1080 SNSAQIYGNS IILKKPIKYS PLKDCIYTNL LYFKSIYSVN SFDVIIATQM SSSVSPSSLS 1090 1100 1110 1120 1130 1140 SSPSIQGLTN SSLSINNINI SGNNSNNNIN NNNNNSGSST PKKLKKSNSD QSIHFSPSLT 1150 1160 1170 1180 1190 1200 SSSLPSLDLN NNNNINNNNN INNNNNINNN NNNNNNNNNN NNNNNNNLNH YNSDSILSSD 1210 1220 1230 1240 1250 1260 LSPQQHQYHH PNPLLANYQK KRRNSVVNDT DIPLEMTGIR YPLKIMVAED SLVNQKVACR 1270 1280 1290 1300 1310 1320 FLTKLGYKKE EIIFVVNGQQ AIDHIENVEM VDVILMDMQM PEVDGCEATT RIRMRYPTTG 1330 1340 1350 1360 1370 PHIIGLTANA FNEDKDKCLL SGMCHYLAKP VKMDILAVEL KRAWLIRNKF RVCLCAVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The histidine kinases of Dictyostelium." Anjard C., Loomis W.F. (In) Inouye M., Dutta R. (eds.); Histidine kinases in signal transduction, pp.1-1, Academic press, San Diego (2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-1378. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000035 Genomic DNA. Translation: EAL67428.1. AF362370 Genomic DNA. Translation: AAK54089.2. Frameshift.
RefSeq	XP_641414.1. XM_636322.1.
3D structure databases
HSSP	HSSP built from PDB template 1OXK based on UniProtKB P39928.
ProteinModelPortal	Q54W36.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0219944; DDB0219944; DDB_G0279913.
GeneID	8622297.
GenomeReviews	Gene locus dhkH in contig CM000152_GR.
KEGG	ddi:DDB_G0279913.
Organism-specific databases
dictyBase	DDB_G0279913. dhkH.
Phylogenomic databases
eggNOG	KOG0519.
GeneTree	EPrGT00050000001666.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011006. CheY-like_superfamily. IPR000014. PAS. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 2 hits.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00091. PAS. 1 hit. SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF52172. CheY_like. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. PS50112. PAS. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHKH_DICDI

Accession

Primary (citable) accession number: Q54W36
Secondary accession number(s): Q95PH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)