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Protein

Methionine aminopeptidase 1D, mitochondrial

Gene

metap1d

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232SubstrateUniRule annotation1
Metal bindingi249Divalent metal cation 1UniRule annotation1
Metal bindingi260Divalent metal cation 1UniRule annotation1
Metal bindingi260Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi323Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei330SubstrateUniRule annotation1
Metal bindingi355Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi389Divalent metal cation 1UniRule annotation1
Metal bindingi389Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Protein family/group databases

MEROPSiM24.001

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 1D, mitochondrialUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 1DUniRule annotation
Short name:
MetAP 1DUniRule annotation
Alternative name(s):
Methionyl aminopeptidase type 1D, mitochondrial
Peptidase M 1DUniRule annotation
Gene namesi
Name:metap1d
Synonyms:map1d
ORF Names:DDB_G0280127
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0280127 map1d

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 58MitochondrionUniRule annotationAdd BLAST58
ChainiPRO_000032851459 – 404Methionine aminopeptidase 1D, mitochondrialAdd BLAST346

Proteomic databases

PaxDbiQ54VU7

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0235404

Structurei

3D structure databases

ProteinModelPortaliQ54VU7
SMRiQ54VU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 35Poly-Ser9
Compositional biasi38 – 42Poly-Asn5

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2738 Eukaryota
COG0024 LUCA
InParanoidiQ54VU7
KOiK01265
OMAiDGWMGIL
PhylomeDBiQ54VU7

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54VU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKILKNIIN KSSINNVFKT SFNGGISSSS SSSSSYLNNN NNIIKSYNVQ
60 70 80 90 100
QKQQQRYYSS FEDDLSPKKL KEKILENETE EIRDFVRSQR LTKKTASPLE
110 120 130 140 150
GMNRKERRKM TTKLYRNPDN LIRGGIVSPQ PLIPAHIKKP KYVLGEPVID
160 170 180 190 200
FEIDDPIEIH TAESIEHMRV VGKMAKEVLE YAGTLVRPGI TTDEIDKLVH
210 220 230 240 250
QNIIDRGAYP SPLGYKGFPK SICTSINEVL CHGIPDDRPL EFGDIVKIDV
260 270 280 290 300
TLYYNGYHGD TCATFPVGEI DSSSKRLIEA TEKALYAAIG EVKDGALFNK
310 320 330 340 350
IGKKIQLVAN KYSLSVTPEF TGHGIGQLFH TAPFVFQCAN EFDSVMKEGM
360 370 380 390 400
IFTIEPVLVE STSPYAEWKM WDDKWTVSSR EGGWSAQFEH TILVTKDGYE

ILTK
Length:404
Mass (Da):45,549
Last modified:May 24, 2005 - v1
Checksum:iAC369296B3E58FD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000035 Genomic DNA Translation: EAL67290.1
RefSeqiXP_641258.1, XM_636166.1

Genome annotation databases

EnsemblProtistsiEAL67290; EAL67290; DDB_G0280127
GeneIDi8622390
KEGGiddi:DDB_G0280127

Similar proteinsi

Entry informationi

Entry nameiMAP12_DICDI
AccessioniPrimary (citable) accession number: Q54VU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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