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Q54VQ5

- GADA_DICDI

UniProt

Q54VQ5 - GADA_DICDI

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Protein
Glutamate decarboxylase A
Gene
gadA, DDB_G0280199
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Generates GABA from glutamate. GABA induces the release of acbA from prespore cells and induces the exposure of tagC on the surface of prestalk cells where it can convert acbA to SDF-2. Glutamate acts as a competitive inhibitor.1 Publication

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Pyridoxal phosphate By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621Substrate By similarity
Binding sitei83 – 831Substrate By similarity
Binding sitei210 – 2101Pyridoxal phosphate By similarity
Binding sitei273 – 2731Pyridoxal phosphate By similarity

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: dictyBase
  2. pyridoxal phosphate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glutamate catabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase A (EC:4.1.1.15)
Gene namesi
Name:gadA
ORF Names:DDB_G0280199
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0280199. gadA.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: dictyBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutant cells grow and develop well, forming normally proportioned fruiting bodies, but the number of viable spores is reduced to about half.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Glutamate decarboxylase A
PRO_0000391329Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei274 – 2741N6-(pyridoxal phosphate)lysine By similarity

Expressioni

Tissue specificityi

Exclusively expressed in prespore cells late in development.1 Publication

Developmental stagei

Expressed only after 10 hours of development and its mRNA accumulates to peak at 18 hours when cells are initiating fruiting body formation. Highly expressed during phagocytosis of non-pathogenic bacteria.2 Publications

Interactioni

Subunit structurei

Homohexamer By similarity.

Protein-protein interaction databases

STRINGi44689.DDB_0231446.

Structurei

3D structure databases

ProteinModelPortaliQ54VQ5.
SMRiQ54VQ5. Positions 18-447.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Pyridoxal phosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0076.
InParanoidiQ54VQ5.
KOiK01580.
OMAiKIHERTR.
PhylomeDBiQ54VQ5.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54VQ5-1 [UniParc]FASTAAdd to Basket

« Hide

MSLHHVKTNK YKGFYDSYAS PPATKEISKY SLKEESNKPE VIRDLIIDEL    50
LLDGNAKQNL ATFCQTDLDK EIHVLMDKCI DKNMIDKDEY PQTAEIESRC 100
VHILADLWNS PESSETIGCS TTGSSEAAML GGMALKWRWR ENRKKQGKPF 150
DKPNIVTGPV QICWHKFALY FDIELREVPM EHDRYIMTPE EAIKRCDENT 200
IGVIPTLGVT FTLQYEDVKG ISEALDKFEK ETGLDIPIHV DAASGGFVAP 250
FLQPEIVWDF RLPRVKSING SGHKFGLSPL GVGWVVWRGK NDIHKDLVFD 300
VNYLGGNMPT FSLNFSRPGG QIVCQYYNFL RHGRSGYTAV HQACLDHGIF 350
ISKQVKKHGI FDIVYDGTGA LPGACWKLKK DAKVKFNLFD LSDRMRNRGW 400
QIASYTLPNL PDIVVQRVLI RHGFSHDMAM LLIEDFNRSI EYFDKHPMVQ 450
SISKDEGQSF HH 462
Length:462
Mass (Da):52,621
Last modified:May 24, 2005 - v1
Checksum:i5DFE4AB49DD215CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000035 Genomic DNA. Translation: EAL67326.1.
RefSeqiXP_641300.1. XM_636208.1.

Genome annotation databases

EnsemblProtistsiDDB0231446; DDB0231446; DDB_G0280199.
GeneIDi8622433.
KEGGiddi:DDB_G0280199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000035 Genomic DNA. Translation: EAL67326.1 .
RefSeqi XP_641300.1. XM_636208.1.

3D structure databases

ProteinModelPortali Q54VQ5.
SMRi Q54VQ5. Positions 18-447.
ModBasei Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231446.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231446 ; DDB0231446 ; DDB_G0280199 .
GeneIDi 8622433.
KEGGi ddi:DDB_G0280199.

Organism-specific databases

dictyBasei DDB_G0280199. gadA.

Phylogenomic databases

eggNOGi COG0076.
InParanoidi Q54VQ5.
KOi K01580.
OMAi KIHERTR.
PhylomeDBi Q54VQ5.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
InterProi IPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view ]
PANTHERi PTHR11999:SF1. PTHR11999:SF1. 1 hit.
Pfami PF00282. Pyridoxal_deC. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Expression patterns of cell-type-specific genes in Dictyostelium."
    Iranfar N., Fuller D., Sasik R., Hwa T., Laub M., Loomis W.F.
    Mol. Biol. Cell 12:2590-2600(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  3. "GABA induces terminal differentiation of Dictyostelium through a GABAB receptor."
    Anjard C., Loomis W.F.
    Development 133:2253-2261(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  4. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
    Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
    BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiGADA_DICDI
AccessioniPrimary (citable) accession number: Q54VQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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