Reviewed, UniProtKB/Swiss-Prot Q54VM2 (MDHC_DICDI)

Names and origin

Protein names

Recommended name:
    Probable malate dehydrogenase 3
    EC=1.1.1.37

Gene names

Name:	mdhC
ORF Names:	DDB_G0280255

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Probable malate dehydrogenase 3		PRO_0000312366
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
Sites
	Active site	188	1	Proton acceptor By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	106	1	NAD By similarity
	Binding site	113	1	NAD By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54VM2-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: B98891A299AB53FF	FASTA	333	35,986
	10 20 30 40 50 60 MSNEVINVLI TGAAGQIAYS LIFNVCKGDM FGLDQRIKLH LLDIPQMVDS LKGIVMEIQD 70 80 90 100 110 120 GAYPLIADTV ITADVKEAFT GVHYAILVGA MPRREGMERA DLLKANAAIF KVQGKALAEH 130 140 150 160 170 180 ANKNVKVLVV GNPANTNALI AQVSANGIPK ENFTCLTRLD QNRAKSQIAL KAGVNVKDVH 190 200 210 220 230 240 NVIIWGNHSS TQYPDYRCGY INLSTGKTPI STAIKDEKWL QGEFISTVQK RGAAVIAARK 250 260 270 280 290 300 LSSAASAAKA ITDHMHDWVL GTAEGEYVSM GVYSDGSYGV PEGLIFSFPV KCANGKYTIV 310 320 330 QGLQMDDLSK NLINLTTEEL VAEKTTALQF LSE

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000035 Genomic DNA. Translation: EAL67354.1.
RefSeq	XP_641333.1.
3D structure databases
HSSP	HSSP built from PDB template 4MDH based on UniProtKB P11708.
SMR	Q54VM2. Positions 5-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54VM2.
Genome annotation databases
GeneID	8622467.
KEGG	ddi:DDB_0230187.
Organism-specific databases
dictyBase	DDB_G0280255. mdhC.
Phylogenomic databases
eggNOG	KOG1496.
HOGENOM	HBG289884.
OMA	DQPIILV.
PhylomeDB	Q54VM2.
Enzyme and pathway databases
BRENDA	1.1.1.37. 424.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR011274. Malate_DH_NAD-dep_euk. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHC_DICDI

Accession

Primary (citable) accession number: Q54VM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)