Reviewed, UniProtKB/Swiss-Prot Q54VI4 (GSTA3_DICDI)

Names and origin

Protein names

Recommended name:
    Putative glutathione S-transferase alpha-3
    EC=2.5.1.18
Alternative name(s):
    GST class-alpha 3

Gene names

Name:	gsta3
ORF Names:	DDB_G0280317

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 204	203	Putative glutathione S-transferase alpha-3		PRO_0000350740
Regions
	Domain	2 – 79	78	GST N-terminal
	Domain	81 – 202	122	GST C-terminal
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q54VI4-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 16D7B61EDC21DCDA	FASTA	204	23,606
	10 20 30 40 50 60 MTKPQLSYFK VRALGQFPRV LLSYLSIDYD NNYIDKIDEN IIDDLKYGQL PLYTDSNGFK 70 80 90 100 110 120 LVQSMAISKY IASQHDFVGK TPEEKALVDE TLAAVNIDVF TFIIRVFRGV EEKEKIQEII 130 140 150 160 170 180 IPRFFAKWNQ ILGEKKYLAG GNSYTLADLY VYVAYEYIGY VLPFAADLLY NGKFPHLDSL 190 200 KEHFESNKGV AEYLKNRPIT ERKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Patel H., Brunton V.G., Frame M.C. Submitted (APR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10; 37-80; 114-123; 128-135 AND 174-188, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000035 Genomic DNA. Translation: EAL67385.1.
RefSeq	XP_641371.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3392446.
GenomeReviews	Gene locus gsta3 in contig CM000152_GR.
KEGG	ddi:DDB_0231429.
Organism-specific databases
dictyBase	DDB_G0280317. gsta3.
Phylogenomic databases
OMA	NIAHNIE.
Family and domain databases
InterPro	IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA3_DICDI

Accession

Primary (citable) accession number: Q54VI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)