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Q54VA2 (FUMH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase, mitochondrial

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:fumH
ORF Names:DDB_G0280495
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O. HAMAP-Rule MF_00743

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP-Rule MF_00743

Subcellular location

Isoform 1: Mitochondrion By similarity HAMAP-Rule MF_00743.

Isoform 2: Cytoplasm By similarity HAMAP-Rule MF_00743.

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q54VA2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q54VA2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1919Mitochondrion Potential
Chain20 – 485466Fumarate hydratase, mitochondrial HAMAP-Rule MF_00743
PRO_0000331360

Regions

Region150 – 1534B site By similarity
Region160 – 1623Substrate binding By similarity

Sites

Binding site1201Substrate By similarity

Natural variations

Alternative sequence1 – 1919Missing in isoform 2.
VSP_033185

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: A70941925A48FBBE

FASTA48552,963
        10         20         30         40         50         60 
MLSASRKLNN QQFLKTIRNM TTFRSEFDTF GEVKVNDEKY WGAQTQRSLE NFDIGGESEK 

        70         80         90        100        110        120 
MPLMVVRSFG ILKRCAAIVN KKYGLDATIA DNIAKAATEV VEGKLDDQFP LVVFQTGSGT 

       130        140        150        160        170        180 
QSNMNANEVI SNRAIELMTG KRDFSKKLVH PNDHVNKSQS SNDTFPTCMH IAAAISINEK 

       190        200        210        220        230        240 
LVPALEMLLA AMRTKQNEFN HIIKIGRTHL QDATPLTLGQ EFSGYCTQIE YGIQRIKDTL 

       250        260        270        280        290        300 
PRLYNLAQGG TAVGTGLNTP VGFDVDIASE VAKFTGLPFK TAPNKFEALA AHDAMVEVSG 

       310        320        330        340        350        360 
ALNTVAVSLM KIANDIRFLG SGPRCGLGEL ILPENEPGSS IMPGKVNPTQ CEAMTMVCAQ 

       370        380        390        400        410        420 
VMGNNTTVSI AGSNGHFELN VFKPVIIKNV LSSIRLIADA SVSFTKHCVV GIKADEKRID 

       430        440        450        460        470        480 
QLLHESLMLV TALNPYIGYD KAAKAAKKAH KEKTTLKEAC LSLGFTTSEE FDKWVDPSKM 


IGSMK 

« Hide

Isoform 2 [UniParc].

Checksum: F428A15A5F9FB22B
Show »

FASTA46650,718

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000036 Genomic DNA. Translation: EAL67226.1.
AAFI02000036 Genomic DNA. Translation: EAL67227.1.

3D structure databases

ProteinModelPortalQ54VA2.
SMRQ54VA2. Positions 23-482.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231397.

Proteomic databases

PRIDEQ54VA2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231397; DDB0231397; DDB_G0280495. [Q54VA2-1]
KEGGddi:DDB_G0280495.

Organism-specific databases

dictyBaseDDB_G0280495. fumH.

Phylogenomic databases

eggNOGCOG0114.
KOK01679.
OMARIEKDTM.
PhylomeDBQ54VA2.
ProtClustDBCLSZ2430620.

Enzyme and pathway databases

UniPathwayUPA00223; UER01007.

Family and domain databases

Gene3D1.10.275.10. 1 hit.
HAMAPMF_00743. FumaraseC.
InterProIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERPTHR11444. PTHR11444. 1 hit.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
SUPFAMSSF48557. SSF48557. 1 hit.
TIGRFAMsTIGR00979. fumC_II. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54VA2.

Entry information

Entry nameFUMH_DICDI
AccessionPrimary (citable) accession number: Q54VA2
Secondary accession number(s): Q54VA1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase