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Q54VA2

- FUMH_DICDI

UniProt

Q54VA2 - FUMH_DICDI

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Protein

Fumarate hydratase, mitochondrial

Gene

fumH

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-malate = fumarate + H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201SubstrateBy similarity

GO - Molecular functioni

  1. fumarate hydratase activity Source: dictyBase

GO - Biological processi

  1. fumarate metabolic process Source: dictyBase
  2. response to bacterium Source: dictyBase
  3. tricarboxylic acid cycle Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase, mitochondrial (EC:4.2.1.2)
Short name:
Fumarase
Gene namesi
Name:fumH
ORF Names:DDB_G0280495
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0280495. fumH.

Subcellular locationi

Isoform 1 : Mitochondrion By similarity
Isoform 2 : Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
  2. mitochondrion Source: dictyBase
  3. phagocytic vesicle Source: dictyBase
  4. tricarboxylic acid cycle enzyme complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1919MitochondrionSequence AnalysisAdd
BLAST
Chaini20 – 485466Fumarate hydratase, mitochondrialPRO_0000331360Add
BLAST

Proteomic databases

PRIDEiQ54VA2.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231397.

Structurei

3D structure databases

ProteinModelPortaliQ54VA2.
SMRiQ54VA2. Positions 23-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1534B siteBy similarity
Regioni160 – 1623Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0114.
InParanoidiQ54VA2.
KOiK01679.
OMAiMESFNIH.
PhylomeDBiQ54VA2.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q54VA2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSASRKLNN QQFLKTIRNM TTFRSEFDTF GEVKVNDEKY WGAQTQRSLE
60 70 80 90 100
NFDIGGESEK MPLMVVRSFG ILKRCAAIVN KKYGLDATIA DNIAKAATEV
110 120 130 140 150
VEGKLDDQFP LVVFQTGSGT QSNMNANEVI SNRAIELMTG KRDFSKKLVH
160 170 180 190 200
PNDHVNKSQS SNDTFPTCMH IAAAISINEK LVPALEMLLA AMRTKQNEFN
210 220 230 240 250
HIIKIGRTHL QDATPLTLGQ EFSGYCTQIE YGIQRIKDTL PRLYNLAQGG
260 270 280 290 300
TAVGTGLNTP VGFDVDIASE VAKFTGLPFK TAPNKFEALA AHDAMVEVSG
310 320 330 340 350
ALNTVAVSLM KIANDIRFLG SGPRCGLGEL ILPENEPGSS IMPGKVNPTQ
360 370 380 390 400
CEAMTMVCAQ VMGNNTTVSI AGSNGHFELN VFKPVIIKNV LSSIRLIADA
410 420 430 440 450
SVSFTKHCVV GIKADEKRID QLLHESLMLV TALNPYIGYD KAAKAAKKAH
460 470 480
KEKTTLKEAC LSLGFTTSEE FDKWVDPSKM IGSMK
Length:485
Mass (Da):52,963
Last modified:May 24, 2005 - v1
Checksum:iA70941925A48FBBE
GO
Isoform 2 (identifier: Q54VA2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Show »
Length:466
Mass (Da):50,718
Checksum:iF428A15A5F9FB22B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919Missing in isoform 2. CuratedVSP_033185Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000036 Genomic DNA. Translation: EAL67226.1.
AAFI02000036 Genomic DNA. Translation: EAL67227.1.
RefSeqiXP_001134561.1. XM_001134561.1.
XP_641207.1. XM_636115.1.

Genome annotation databases

EnsemblProtistsiDDB0231397; DDB0231397; DDB_G0280495. [Q54VA2-1]
GeneIDi8622588.
KEGGiddi:DDB_G0280495.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000036 Genomic DNA. Translation: EAL67226.1 .
AAFI02000036 Genomic DNA. Translation: EAL67227.1 .
RefSeqi XP_001134561.1. XM_001134561.1.
XP_641207.1. XM_636115.1.

3D structure databases

ProteinModelPortali Q54VA2.
SMRi Q54VA2. Positions 23-482.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231397.

Proteomic databases

PRIDEi Q54VA2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231397 ; DDB0231397 ; DDB_G0280495 . [Q54VA2-1 ]
GeneIDi 8622588.
KEGGi ddi:DDB_G0280495.

Organism-specific databases

dictyBasei DDB_G0280495. fumH.

Phylogenomic databases

eggNOGi COG0114.
InParanoidi Q54VA2.
KOi K01679.
OMAi MESFNIH.
PhylomeDBi Q54VA2.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01007 .

Miscellaneous databases

PROi Q54VA2.

Family and domain databases

Gene3Di 1.10.275.10. 1 hit.
HAMAPi MF_00743. FumaraseC.
InterProi IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view ]
PANTHERi PTHR11444. PTHR11444. 1 hit.
Pfami PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view ]
PRINTSi PR00149. FUMRATELYASE.
SUPFAMi SSF48557. SSF48557. 1 hit.
TIGRFAMsi TIGR00979. fumC_II. 1 hit.
PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiFUMH_DICDI
AccessioniPrimary (citable) accession number: Q54VA2
Secondary accession number(s): Q54VA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3