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Q54UT2

- DGK_DICDI

UniProt

Q54UT2 - DGK_DICDI

Protein

Deoxyguanosine kinase

Gene

dgk

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Purine-specific deoxyribonuleoside kinase that phosphorylates preferentially deoxyguanosine, as part of the deoxyribonucleotide salvage pathway.

    Catalytic activityi

    ATP + deoxyguanosine = ADP + dGMP.1 Publication

    Kineticsi

    1. KM=1.4 µM for deoxyguanosine1 Publication
    2. KM=206 µM for deoxyadenosine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631SubstrateBy similarity
    Binding sitei75 – 751SubstrateBy similarity
    Binding sitei86 – 861SubstrateBy similarity
    Active sitei114 – 1141Proton acceptorSequence Analysis
    Binding sitei115 – 1151SubstrateBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei180 – 1801SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 479ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. deoxyguanosine kinase activity Source: dictyBase
    3. phosphotransferase activity, alcohol group as acceptor Source: InterPro

    GO - Biological processi

    1. deoxyguanosine salvage Source: dictyBase
    2. deoxyribonucleoside monophosphate biosynthetic process Source: GOC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKQ54UT2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyguanosine kinase (EC:2.7.1.113)
    Short name:
    dGK
    Alternative name(s):
    DddGK
    Gene namesi
    Name:dgk
    ORF Names:DDB_G0280843
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0280843. dgk.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Deoxyguanosine kinasePRO_0000327522Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0218264.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54UT2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi272 – 2798Poly-Asn

    Sequence similaritiesi

    Belongs to the DCK/DGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1428.
    KOiK03954.
    OMAiWVFAENC.
    PhylomeDBiQ54UT2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q54UT2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFRRSLMFMI SNNKNTNMVS SINTTNKVNN FSKIIILEGN ISAGKTYLSS    50
    KLGDLLGYKV FLEPTATNPY LSLFYKEPSK YALIMQKWLL NQRYNTFLNA 100
    LQYSLENEQG VILDRSVYSD WVFAENCRSE GLISAEGFKE YNSIRDRFLS 150
    NIPIPNVTLF LDVDPKQCLQ RIQNRKRDCE QSIPLSYLSG LDNCYKKFLI 200
    EMKSKGSNVI ILDWNNFGDI NLVLNEINND NFNNFNNSNN SKFNDVNYKK 250
    QQLISDIENE KNNLKEMKFF LNENNNNNNQ EKIKS 285
    Length:285
    Mass (Da):33,193
    Last modified:March 18, 2008 - v2
    Checksum:i1857E13F82E733C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY669384 mRNA. Translation: AAV85947.1.
    AAFI02000025 Genomic DNA. Translation: EAL67033.2.
    RefSeqiXP_641009.2. XM_635917.2.

    Genome annotation databases

    EnsemblProtistsiDDB0233982; DDB0233982; DDB_G0280843.
    GeneIDi8621793.
    KEGGiddi:DDB_G0280843.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY669384 mRNA. Translation: AAV85947.1 .
    AAFI02000025 Genomic DNA. Translation: EAL67033.2 .
    RefSeqi XP_641009.2. XM_635917.2.

    3D structure databases

    ProteinModelPortali Q54UT2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0218264.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0233982 ; DDB0233982 ; DDB_G0280843 .
    GeneIDi 8621793.
    KEGGi ddi:DDB_G0280843.

    Organism-specific databases

    dictyBasei DDB_G0280843. dgk.

    Phylogenomic databases

    eggNOGi COG1428.
    KOi K03954.
    OMAi WVFAENC.
    PhylomeDBi Q54UT2.

    Enzyme and pathway databases

    SABIO-RK Q54UT2.

    Miscellaneous databases

    PROi Q54UT2.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10513. PTHR10513. 1 hit.
    Pfami PF01712. dNK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
      Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
      J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiDGK_DICDI
    AccessioniPrimary (citable) accession number: Q54UT2
    Secondary accession number(s): Q14EL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 54 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3