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Protein

Deoxyguanosine kinase

Gene

dgk

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Purine-specific deoxyribonuleoside kinase that phosphorylates preferentially deoxyguanosine, as part of the deoxyribonucleotide salvage pathway.

Catalytic activityi

ATP + deoxyguanosine = ADP + dGMP.1 Publication

Kineticsi

  1. KM=1.4 µM for deoxyguanosine1 Publication
  2. KM=206 µM for deoxyadenosine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631SubstrateBy similarity
    Binding sitei75 – 751SubstrateBy similarity
    Binding sitei86 – 861SubstrateBy similarity
    Active sitei114 – 1141Proton acceptorSequence Analysis
    Binding sitei115 – 1151SubstrateBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei180 – 1801SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 479ATPBy similarity

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • deoxyguanosine kinase activity Source: dictyBase

    GO - Biological processi

    • deoxyguanosine salvage Source: dictyBase
    • deoxyribonucleoside monophosphate biosynthetic process Source: GOC
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.113. 1939.
    SABIO-RKQ54UT2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyguanosine kinase (EC:2.7.1.113)
    Short name:
    dGK
    Alternative name(s):
    DddGK
    Gene namesi
    Name:dgk
    ORF Names:DDB_G0280843
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0280843. dgk.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Deoxyguanosine kinasePRO_0000327522Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB0233982.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54UT2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi272 – 2798Poly-Asn

    Sequence similaritiesi

    Belongs to the DCK/DGK family.Curated

    Phylogenomic databases

    eggNOGiCOG1428.
    InParanoidiQ54UT2.
    KOiK03954.
    OMAiHRDYLDE.
    PhylomeDBiQ54UT2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000705. DNK. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q54UT2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFRRSLMFMI SNNKNTNMVS SINTTNKVNN FSKIIILEGN ISAGKTYLSS
    60 70 80 90 100
    KLGDLLGYKV FLEPTATNPY LSLFYKEPSK YALIMQKWLL NQRYNTFLNA
    110 120 130 140 150
    LQYSLENEQG VILDRSVYSD WVFAENCRSE GLISAEGFKE YNSIRDRFLS
    160 170 180 190 200
    NIPIPNVTLF LDVDPKQCLQ RIQNRKRDCE QSIPLSYLSG LDNCYKKFLI
    210 220 230 240 250
    EMKSKGSNVI ILDWNNFGDI NLVLNEINND NFNNFNNSNN SKFNDVNYKK
    260 270 280
    QQLISDIENE KNNLKEMKFF LNENNNNNNQ EKIKS
    Length:285
    Mass (Da):33,193
    Last modified:March 18, 2008 - v2
    Checksum:i1857E13F82E733C5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY669384 mRNA. Translation: AAV85947.1.
    AAFI02000025 Genomic DNA. Translation: EAL67033.2.
    RefSeqiXP_641009.2. XM_635917.2.

    Genome annotation databases

    EnsemblProtistsiDDB0233982; DDB0233982; DDB_G0280843.
    GeneIDi8621793.
    KEGGiddi:DDB_G0280843.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY669384 mRNA. Translation: AAV85947.1.
    AAFI02000025 Genomic DNA. Translation: EAL67033.2.
    RefSeqiXP_641009.2. XM_635917.2.

    3D structure databases

    ProteinModelPortaliQ54UT2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi44689.DDB0233982.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblProtistsiDDB0233982; DDB0233982; DDB_G0280843.
    GeneIDi8621793.
    KEGGiddi:DDB_G0280843.

    Organism-specific databases

    dictyBaseiDDB_G0280843. dgk.

    Phylogenomic databases

    eggNOGiCOG1428.
    InParanoidiQ54UT2.
    KOiK03954.
    OMAiHRDYLDE.
    PhylomeDBiQ54UT2.

    Enzyme and pathway databases

    BRENDAi2.7.1.113. 1939.
    SABIO-RKQ54UT2.

    Miscellaneous databases

    PROiQ54UT2.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR002624. DNK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10513. PTHR10513. 1 hit.
    PfamiPF01712. dNK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000705. DNK. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
      Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
      J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiDGK_DICDI
    AccessioniPrimary (citable) accession number: Q54UT2
    Secondary accession number(s): Q14EL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: March 18, 2008
    Last modified: June 24, 2015
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.