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Q54UT2 (DGK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyguanosine kinase

Short name=dGK
EC=2.7.1.113
Alternative name(s):
DddGK
Gene names
Name:dgk
ORF Names:DDB_G0280843
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Purine-specific deoxyribonuleoside kinase that phosphorylates preferentially deoxyguanosine, as part of the deoxyribonucleotide salvage pathway.

Catalytic activity

ATP + deoxyguanosine = ADP + dGMP. Ref.1

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the DCK/DGK family.

Biophysicochemical properties

Kinetic parameters:

KM=1.4 µM for deoxyguanosine Ref.1

KM=206 µM for deoxyadenosine

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Deoxyguanosine kinase
PRO_0000327522

Regions

Nucleotide binding39 – 479ATP By similarity
Compositional bias272 – 2798Poly-Asn

Sites

Active site1141Proton acceptor Potential
Binding site631Substrate By similarity
Binding site751Substrate By similarity
Binding site861Substrate By similarity
Binding site1151Substrate By similarity
Binding site1201Substrate By similarity
Binding site1801Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54UT2 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: 1857E13F82E733C5

FASTA28533,193
        10         20         30         40         50         60 
MFRRSLMFMI SNNKNTNMVS SINTTNKVNN FSKIIILEGN ISAGKTYLSS KLGDLLGYKV 

        70         80         90        100        110        120 
FLEPTATNPY LSLFYKEPSK YALIMQKWLL NQRYNTFLNA LQYSLENEQG VILDRSVYSD 

       130        140        150        160        170        180 
WVFAENCRSE GLISAEGFKE YNSIRDRFLS NIPIPNVTLF LDVDPKQCLQ RIQNRKRDCE 

       190        200        210        220        230        240 
QSIPLSYLSG LDNCYKKFLI EMKSKGSNVI ILDWNNFGDI NLVLNEINND NFNNFNNSNN 

       250        260        270        280 
SKFNDVNYKK QQLISDIENE KNNLKEMKFF LNENNNNNNQ EKIKS 

« Hide

References

« Hide 'large scale' references
[1]"Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases."
Sandrini M.P.B., Soederbom F., Mikkelsen N.E., Piskur J.
J. Mol. Biol. 369:653-664(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY669384 mRNA. Translation: AAV85947.1.
AAFI02000025 Genomic DNA. Translation: EAL67033.2.

3D structure databases

ProteinModelPortalQ54UT2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0218264.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0233982; DDB0233982; DDB_G0280843.
KEGGddi:DDB_G0280843.

Organism-specific databases

dictyBaseDDB_G0280843. dgk.

Phylogenomic databases

eggNOGCOG1428.
OMAHRDYLDE.
PhylomeDBQ54UT2.
ProtClustDBCLSZ2430595.

Enzyme and pathway databases

SABIO-RKQ54UT2.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR002624. Deoxynucleoside_kinase.
IPR015828. NADH_UbQ_OxRdtase_42KD_su.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10513. PTHR10513. 1 hit.
PTHR10513:SF2. PTHR10513:SF2. 1 hit.
PfamPF01712. dNK. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

PROQ54UT2.

Entry information

Entry nameDGK_DICDI
AccessionPrimary (citable) accession number: Q54UT2
Secondary accession number(s): Q14EL5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 18, 2008
Last modified: April 16, 2014
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase