Reviewed, UniProtKB/Swiss-Prot Q54UH8 (SERA_DICDI)

Names and origin

Protein names

Recommended name:
    D-3-phosphoglycerate dehydrogenase
      Short name=3-PGDH
    EC=1.1.1.95

Gene names

Name:	serA
ORF Names:	DDB_G0281071

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	D-3-phosphoglycerate dehydrogenase		PRO_0000328330
Regions
	Domain	339 – 399	61	ACT
Sites
	Active site	240	1	By similarity
	Active site	269	1	By similarity
	Active site	292	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54UH8-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 3A0EDFB5796ED000	FASTA	407	44,749
	10 20 30 40 50 60 MDPTTSFPKD KIKILLLENI HIAAIKQFEE QGFQVESISS SLPEDKIIEK IKDVHVLGLR 70 80 90 100 110 120 SKTKVTEKIL SEAKRLLAIG CFCIGTDQVD LIEAEKRGVP VFNSPFCNSR SVAELIICEI 130 140 150 160 170 180 ITLSRKLGDR STEMHNKIWR KESANCHEIR GKTLGIIGYG HIGSQLSVLA EAMGMSVLYY 190 200 210 220 230 240 DIARRLPLGN SKMCPDMKTL LENSNFVTLH VPDTKETVGL IGEEEINTMK KGSYLLNASR 250 260 270 280 290 300 GKVVQIPHLA NALRSGHLAG AAVDVYPEEP SANCKDWECE LQKCPNTILT PHIGGSTEEA 310 320 330 340 350 360 QEAIGLEVSD LIVQFINSGA SAGSVNFPEI ALPVSPSTHR ILNIHNNKPG VLRDINNILS 370 380 390 400 EFNVSAQVLS TRKQIGYIIA DVDSEASKEI KKKISSLPNS IKTRVLY

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000040 Genomic DNA. Translation: EAL66832.1.
RefSeq	XP_640798.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3391863.
KEGG	ddi:DDB_0230052.
Organism-specific databases
dictyBase	DDB_G0281071. serA.
Phylogenomic databases
OMA	Q54UH8. TEGELGY.
Enzyme and pathway databases
BRENDA	1.1.1.95. 424.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF01842. ACT. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_DICDI

Accession

Primary (citable) accession number: Q54UH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)