Q54UF7 (LDHD_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative D-lactate dehydrogenase
Short name=D-LDH
EC=1.1.1.28

Gene names

Name:	ldhA
ORF Names:	DDB_G0281101

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lactate fermentation to propionate and acetate Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	phagocytic vesicle Inferred from direct assay Ref.2. Source: dictyBase
Molecular function	D-lactate dehydrogenase activity Inferred from sequence or structural similarity. Source: dictyBase NAD binding Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Putative D-lactate dehydrogenase		PRO_0000312345
Regions
	Nucleotide binding	153 – 154	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity
	Binding site	206	1	NAD; via carbonyl oxygen By similarity
	Binding site	259	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54UF7 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 375261C87FBACC69	FASTA	340	37,907
	10 20 30 40 50 60 MKITLFSSKP YWVKWFNELN KFSYEINYVT SACDIKSVNE AKGSEAVCCF VNDDLSKEVI 70 80 90 100 110 120 ETLHSNGTKV ILMRCAGFNK VDLDTANKLG IPVLRVPAYS PNAVSEYALS LIMALNRKTH 130 140 150 160 170 180 KAHDRVRDAN FEINGMEGFN MVSKVYGIVG TGNIGEQLCR VLKLGFGAKV IAYDIIENKA 190 200 210 220 230 240 VTDIGIEYVK TLDEIWKQCD VISLHTPLNS QTKYMVNSES IEKMRDGVMI INVSRGALVN 250 260 270 280 290 300 ASDAIVGLKS GKISSLGMDV YENETDYFYQ DHNGSIIKDD NLSLLISYPN VMITSHQAWY 310 320 330 340 TKEAISCICG TSLQNFVDFR SNQIKKSNLV NNPISSQPTQ

References

[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000040 Genomic DNA. Translation: EAL66847.1.
RefSeq	XP_640819.1. XM_635727.1.
3D structure databases
HSSP	HSSP built from PDB template 1J4A based on UniProtKB P26297.
ProteinModelPortal	Q54UF7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54UF7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0238140; DDB0238140; DDB_G0281101.
GeneID	8622873.
GenomeReviews	Gene locus ldhA in contig CM000152_GR.
KEGG	ddi:DDB_G0281101.
Organism-specific databases
dictyBase	DDB_G0281101. ldhA.
Phylogenomic databases
eggNOG	KOG0068.
GeneTree	EPrGT00050000005269.
HOGENOM	HBG731446.
OMA	ANDNINK.
PhylomeDB	Q54UF7.
ProtClustDB	CLSZ2430567.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_DICDI

Accession

Primary (citable) accession number: Q54UF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)