Reviewed, UniProtKB/Swiss-Prot Q54U72 (IMPA1_DICDI)

Names and origin

Protein names

Recommended name:
    Inositol monophosphatase
    EC=3.1.3.25
Alternative name(s):
    Inositol-1(or 4)-monophosphatase
      Short name=IMPase
      Short name=IMP

Gene names

Name:	impa1
ORF Names:	DDB_G0281239

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides By similarity.
Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inhibited by Li+ By similarity.
Pathway	Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	inositol-1(or 4)-monophosphatase activity Inferred from electronic annotation. Source: EC lithium ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Inositol monophosphatase		PRO_0000328360
Regions
	Region	92 – 95	4	Substrate binding By similarity
Sites
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 1 By similarity
	Metal binding	90	1	Magnesium 2 By similarity
	Metal binding	93	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	93	1	Magnesium 2 By similarity
	Metal binding	220	1	Magnesium 2 By similarity
	Binding site	71	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54U72-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 6C21EF15B750AFFD	FASTA	272	29,899
	10 20 30 40 50 60 MENITLDQYL QSAVDVVKEI GPMILKNYNS RSKQIEYKGA IDLVTDTDKA VEEHIIKTLT 70 80 90 100 110 120 TKYPHTKILG EESTKDGIYN WGNEPTWVID PIDGTTNFVH RFPLFCVSIA LSINKEIVVA 130 140 150 160 170 180 CLYAPVLDEL FTATKGGGAF LNGESISVSS VEHLSQSIIS TNVGYDRSDK GIEFMLTNFK 190 200 210 220 230 240 NILKDNVQAL RFSGTAAWEM ASVSCGRVDS FYEWGIHPWD IAAASLLITE AGGVVVDPSG 250 260 270 GKCDMESRKV LCGNPNIVNK LSKLLIEKPT SN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000040 Genomic DNA. Translation: EAL66916.1.
RefSeq	XP_640904.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54U72.
Genome annotation databases
GeneID	3391991.
GenomeReviews	Gene locus impa1 in contig CM000152_GR.
KEGG	ddi:DDB_0235253.
Organism-specific databases
dictyBase	DDB_G0281239. impa1.
Phylogenomic databases
OMA	CQGSAAL.
Enzyme and pathway databases
BRENDA	3.1.3.25. 424.
Family and domain databases
InterPro	IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES.
ProDom	PD023420. Inositol_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMPA1_DICDI

Accession

Primary (citable) accession number: Q54U72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)