Q54U65 (7TMK2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Seven transmembrane domain-containing serine/threonine-protein kinase 2
EC=2.7.11.1

Gene names

Name:	7tmk2
ORF Names:	DDB_G0281331

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Seven transmembrane domain-containing serine/threonine-protein kinase 2		PRO_0000362005
Regions
	Topological domain	1 – 5	5	Extracellular Potential
	Transmembrane	6 – 26	21	Helical; Name=1; Potential
	Topological domain	27 – 42	16	Cytoplasmic Potential
	Transmembrane	43 – 63	21	Helical; Name=2; Potential
	Topological domain	64 – 76	13	Extracellular Potential
	Transmembrane	77 – 97	21	Helical; Name=3; Potential
	Topological domain	98 – 121	24	Cytoplasmic Potential
	Transmembrane	122 – 142	21	Helical; Name=4; Potential
	Topological domain	143 – 185	43	Extracellular Potential
	Transmembrane	186 – 206	21	Helical; Name=5; Potential
	Topological domain	207 – 224	18	Cytoplasmic Potential
	Transmembrane	225 – 245	21	Helical; Name=6; Potential
	Topological domain	246 – 265	20	Extracellular Potential
	Transmembrane	266 – 286	21	Helical; Name=7; Potential
	Topological domain	287 – 704	418	Cytoplasmic Potential
	Domain	317 – 682	366	Protein kinase
	Nucleotide binding	323 – 331	9	ATP By similarity
	Compositional bias	453 – 460	8	Poly-Asn
	Compositional bias	465 – 471	7	Poly-Gln
	Compositional bias	563 – 568	6	Poly-Thr
Sites
	Active site	506	1	Proton acceptor By similarity
	Binding site	350	1	ATP By similarity
Amino acid modifications
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54U65 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: B7C0D02A3387EA5B	FASTA	704	80,382
	10 20 30 40 50 60 MPSKEFIIPL ILLCFYSVNG FVAVISSLVE LFIHKASWNS IKIFFYSLLI LQCLCRCIII 70 80 90 100 110 120 GWGMIETVQG GEFYSNFPSL LFISYAGLVA LQMIQFLPND NQYLLLSEGK KNNHKVKVGT 130 140 150 160 170 180 NILIFFNLFM YFGMFLLFGI AEKQVGNSTS FNHHGNHNST TSTSTDEIPL VSTEVGELYL 190 200 210 220 230 240 FGDKDPIYIV LDCFYFVCLL LLLIFHSYVG WKTYKRNKDL FGIKLNVIHL ILLICIFIRS 250 260 270 280 290 300 LLVIIDPSSP NNSILHIDTE SWLIYIYTIS YYVVGEIIPG MLLIVIEFLL PYHKRKDFIN 310 320 330 340 350 360 IGGELSSYQD VWKSENIAIH ELLGMGGSGA MVHKCTVKKG PLRGGTFAVK VMKDCTNEDI 370 380 390 400 410 420 ESLENEIRVY EKLKSPYIVS YQGSSKVVGS SGQIFEIRLF MEYIPHTLDK YLLARSVCGD 430 440 450 460 470 480 SNGGGSSRNL KNYFLHMQEY QSSPQSSISY YQNNNNNNNN SPLIQPQQQQ QYVYPYYFRY 490 500 510 520 530 540 SQVVWYLYQI SIGLDNLHAN KIAHRDLKSN NIFVTLSESE VKICKIGDFD ISRSFNNPKV 550 560 570 580 590 600 LNVLSNPTAL QQQKLNNFFQ SATTTTTTEQ TTEPIEVAAT TTPQTIHSAF QHDILSFGFI 610 620 630 640 650 660 VLDFLTLSNY SCFDSKFDTS NYYDDDKFNN KSNTKIKKPD LPDYIKKDEK LWEPVIQLYK 670 680 690 700 SCTSDDPNKR PSSLGVKFHL ANLYKDIIES GTEVWSIEKD SSSK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000040 Genomic DNA. Translation: EAL66962.1.
RefSeq	XP_640911.1. XM_635819.1.
3D structure databases
ProteinModelPortal	Q54U65.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0220006; DDB0220006; DDB_G0281331.
GeneID	8622973.
GenomeReviews	Gene locus 7tmk2 in contig CM000152_GR.
KEGG	ddi:DDB_G0281331.
Organism-specific databases
dictyBase	DDB_G0281331.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

7TMK2_DICDI

Accession

Primary (citable) accession number: Q54U65

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)