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Protein

NEDD8-conjugating enzyme Ubc12

Gene

ube2m

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Accepts the ubiquitin-like protein nedd8 from the uba3-nae1 E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei159Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-8951664 Neddylation
R-DDI-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00885

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-conjugating enzyme Ubc12 (EC:2.3.2.-)
Alternative name(s):
NEDD8 carrier protein
Ubiquitin-conjugating enzyme E2M
Gene namesi
Name:ube2m
ORF Names:DDB_G0281725
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0281725 ube2m

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003283981 – 230NEDD8-conjugating enzyme Ubc12Add BLAST230

Proteomic databases

PaxDbiQ54TI6

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0238042

Structurei

3D structure databases

ProteinModelPortaliQ54TI6
SMRiQ54TI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 20Poly-Gln10

Domaini

Both the N-terminal docking peptide and the catalytic core domain must bind the uba3-nae1 complex simultaneously for optimal transfer of nedd8.By similarity

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0420 Eukaryota
ENOG410XS81 LUCA
InParanoidiQ54TI6
KOiK10579
OMAiMIKIWSM
PhylomeDBiQ54TI6

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q54TI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRLKELQKK QQQQQQQQQQ AAAPATNGTD AVTTEPTDVK RQNSNDLKEI
60 70 80 90 100
RKQKSKDSYF SLKTKQSSES GSKRANPAEL RAQKDIDEME VPTGCAVSFK
110 120 130 140 150
DTNDILNFNL SITPTDGLYQ SATFQFTINI PSTYPYDPPK VHCDTLVYHP
160 170 180 190 200
NIDLEGHVCL NILRQDWMPV LNIGTVIFGL MTLFLEPNPD DPLNKDAAQL
210 220 230
MIDNKKTFEA NVRQSLRGGY ISNRQFPKLL
Length:230
Mass (Da):26,066
Last modified:May 24, 2005 - v1
Checksum:i439C54512D5DF5F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000042 Genomic DNA Translation: EAL66624.1
RefSeqiXP_640605.1, XM_635513.1

Genome annotation databases

EnsemblProtistsiEAL66624; EAL66624; DDB_G0281725
GeneIDi8623215
KEGGiddi:DDB_G0281725

Similar proteinsi

Entry informationi

Entry nameiUBC12_DICDI
AccessioniPrimary (citable) accession number: Q54TI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 25, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health