Q54T86 (DWWA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WW domain-containing protein A

Gene names

Name:	dwwA
ORF Names:	DDB_G0281827

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in regulation of actin cytoskeleton organization and cytokinesis. Ref.1
Subunit structure	Interacts with calmodulin in the absence of Ca2+. Ref.1
Subcellular location	Nucleus. Nucleus › nucleolus. Cytoplasm › cell cortex. Cytoplasm › cytoskeleton. Note: In living cells localizes to the cell cortex and nucleus. In formaldehyde fixed cells localizes to the nucleolus. Ref.1
Developmental stage	Expressed during vegetative growth. Expression decreases rapidly during early development and is not detected after 12 hours of development. Ref.1
Disruption phenotype	Cells fail to complete the final step of cytokinesis and form multinucleated cells when grown on a solid surface. Null mutants show excessive accumulation of F-actin around the periphery of the ventral surface. When grown in suspension, mutants lacking dwwA complete cytokinesis normally. Mutants lacking the N-terminal C2 domain fail to localize to the cell cortex. Ref.1
Sequence similarities	Contains 1 C2 domain. Ref.1 Contains 2 WW domains.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Cytoskeleton Nucleus
Domain	Repeat
Ligand	Calmodulin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Inferred from mutant phenotype Ref.1. Source: dictyBase cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis Inferred from mutant phenotype Ref.1. Source: dictyBase protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cell cortex Inferred from direct assay Ref.1. Source: dictyBase cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: dictyBase
Molecular_function	calmodulin binding Inferred from direct assay Ref.1. Source: dictyBase ubiquitin-protein ligase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	WW domain-containing protein A		PRO_0000293628
Regions
	Domain	24 – 144	121	C2 Ref.1
	Domain	325 – 359	35	WW 1
	Domain	519 – 552	34	WW 2
	Compositional bias	380 – 407	28	Poly-Asn
	Compositional bias	497 – 503	7	Poly-Gln
Experimental info
	Sequence conflict	209	1	L → S in BAC56858. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54T86 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: F297EBB82BD15C78	FASTA	568	64,751
	10 20 30 40 50 60 MSNKNPLNNS NGSNSSTIVN SDKFLVKIHS LSVSHTSKSS NIYLVGDFDQ FKQFKTETKK 70 80 90 100 110 120 SSSTHGSCIY NEFAMEFQYE TKYIHKLDHK HFKICVYKKK SIGSDKYIGS FGVDLYTLAT 130 140 150 160 170 180 GPISHDVVFQ KDNTGVGRLQ FRLEMNHITD IQISFKSILL SNLMKQSNYQ IDYSLNKINP 190 200 210 220 230 240 LKSSIIENTI CPSWYNQSSI LMNISLKDLV DGSIFFNLKD TKSSKVIGEL KLPVKSIFSF 250 260 270 280 290 300 VEGDIKIVKT LLYNEKQNKI CDACIEIQFN NIPQLAQLRG GIQTEQGIRG AESFFSGVPL 310 320 330 340 350 360 PKIIGEISNA STSDGYSKEA QLQHVKLPDG WESRIDPVSG KVFYLNHNNK TTSWISPLEH 370 380 390 400 410 420 APVKKRTPTV VISNTTILDN NNNNNNNNNN NNNNNNNNNN NINNTNNIQQ KQQAQQQPVQ 430 440 450 460 470 480 PPPQPVQQQV QQPQQKEKEK EKEINAEDYK ISRPKKTPAT PEEAAVIIQR TFRNHKKQSY 490 500 510 520 530 540 NKTIRNSKTI IPPNNVQQQQ PQQTEKPSIY QVFGQQIDQG LPNGWEVRQD QFGRVFYVDH 550 560 INRATTWTRP TVKHPKQHQQ ATLVQQRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DWWA, a novel protein containing two WW domains and an IQ motif, is required for scission of the residual cytoplasmic bridge during cytokinesis in Dictyostelium." Nagasaki A., Uyeda T.Q.P. Mol. Biol. Cell 15:435-446(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CALMODULIN, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB089315 mRNA. Translation: BAC56858.1. AAFI02000043 Genomic DNA. Translation: EAL66473.1.
RefSeq	XP_640503.1. XM_635411.1.
3D structure databases
HSSP	HSSP built from PDB template 1WR4 based on UniProtKB Q8CFI0.
ProteinModelPortal	Q54T86.
SMR	Q54T86. Positions 520-550.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q54T86. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0216188; DDB0216188; DDB_G0281827.
GeneID	8623318.
KEGG	ddi:DDB_G0281827.
Organism-specific databases
dictyBase	DDB_G0281827. dwwA.
Phylogenomic databases
eggNOG	NOG283080.
OMA	ATGPISH.
ProtClustDB	CLSZ2430519.
Family and domain databases
InterPro	IPR001202. WW_dom. [Graphical view]
Pfam	PF00397. WW. 2 hits. [Graphical view]
SMART	SM00456. WW. 2 hits. [Graphical view]
SUPFAM	SSF51045. WW_Rsp5_WWP. 2 hits.
PROSITE	PS50004. C2. False negative. PS01159. WW_DOMAIN_1. 1 hit. PS50020. WW_DOMAIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DWWA_DICDI

Accession

Primary (citable) accession number: Q54T86
Secondary accession number(s): Q86SB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)