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Protein

Probable ubiquitin carboxyl-terminal hydrolase

Gene

uch1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or nedd8 (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103NucleophileBy similarity1
Active sitei177Proton donorBy similarity1
Sitei192Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DDI-5689603 UCH proteinases
R-DDI-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-DDI-8951664 Neddylation

Protein family/group databases

MEROPSiC12.A11

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase (EC:3.4.19.12)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:uch1
Synonyms:uchl
ORF Names:DDB_G0282007
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282007 uch1

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003311281 – 255Probable ubiquitin carboxyl-terminal hydrolaseAdd BLAST255

Proteomic databases

PaxDbiQ54T48

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0304593

Structurei

3D structure databases

ProteinModelPortaliQ54T48
SMRiQ54T48
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 21Interaction with ubiquitinBy similarity6
Regioni227 – 232Interaction with ubiquitinBy similarity6

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiKOG1415 Eukaryota
ENOG4111HNA LUCA
InParanoidiQ54T48
KOiK05609
OMAiVYGMEPE
PhylomeDBiQ54T48

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
PANTHERiPTHR10589 PTHR10589, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PRINTSiPR00707 UBCTHYDRLASE

Sequencei

Sequence statusi: Complete.

Q54T48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVDQELIET QKNWIPLEAN PEVLTTFMQS LGVSKDWEFC DIYGIDEGLL
60 70 80 90 100
EMVPSPCVAV ILLFPITNEY EDKRYKLEKE IEEKGQVLSD KVYFMKQYIG
110 120 130 140 150
NACGTIGVIH SVLNNANVIE FNENGFFKQF LDKTTSLSTE ERAISLLKNS
160 170 180 190 200
EIEKSHEISA LQGQSNVPQE DEPVVLHFVS FVHVDGHLYE LDGRKPFAIN
210 220 230 240 250
HGESSAETLL KDTANVLQKM IDEDPKEIRF NLMGLVKKPN EESEEEEEKE

KEETK
Length:255
Mass (Da):29,106
Last modified:May 24, 2005 - v1
Checksum:iA0B7E6B2540C24C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000044 Genomic DNA Translation: EAL66425.1
RefSeqiXP_640403.1, XM_635311.1

Genome annotation databases

EnsemblProtistsiEAL66425; EAL66425; DDB_G0282007
GeneIDi8623358
KEGGiddi:DDB_G0282007

Similar proteinsi

Entry informationi

Entry nameiUCHL_DICDI
AccessioniPrimary (citable) accession number: Q54T48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
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