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Q54T48

- UCHL_DICDI

UniProt

Q54T48 - UCHL_DICDI

Protein

Probable ubiquitin carboxyl-terminal hydrolase

Gene

uch1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or nedd8 By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei103 – 1031NucleophileBy similarity
    Active sitei177 – 1771Proton donorBy similarity
    Sitei192 – 1921Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.A11.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ubiquitin carboxyl-terminal hydrolase (EC:3.4.19.12)
    Alternative name(s):
    Ubiquitin thioesterase
    Gene namesi
    Name:uch1
    Synonyms:uchl
    ORF Names:DDB_G0282007
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0282007. uch1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 255255Probable ubiquitin carboxyl-terminal hydrolasePRO_0000331128Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0205083.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54T48.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni16 – 216Interaction with ubiquitinBy similarity
    Regioni227 – 2326Interaction with ubiquitinBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG327708.
    KOiK05609.
    OMAiKEMEVAH.
    PhylomeDBiQ54T48.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.

    Sequencei

    Sequence statusi: Complete.

    Q54T48-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVDQELIET QKNWIPLEAN PEVLTTFMQS LGVSKDWEFC DIYGIDEGLL    50
    EMVPSPCVAV ILLFPITNEY EDKRYKLEKE IEEKGQVLSD KVYFMKQYIG 100
    NACGTIGVIH SVLNNANVIE FNENGFFKQF LDKTTSLSTE ERAISLLKNS 150
    EIEKSHEISA LQGQSNVPQE DEPVVLHFVS FVHVDGHLYE LDGRKPFAIN 200
    HGESSAETLL KDTANVLQKM IDEDPKEIRF NLMGLVKKPN EESEEEEEKE 250
    KEETK 255
    Length:255
    Mass (Da):29,106
    Last modified:May 24, 2005 - v1
    Checksum:iA0B7E6B2540C24C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000044 Genomic DNA. Translation: EAL66425.1.
    RefSeqiXP_640403.1. XM_635311.1.

    Genome annotation databases

    EnsemblProtistsiDDB0304593; DDB0304593; DDB_G0282007.
    GeneIDi8623358.
    KEGGiddi:DDB_G0282007.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000044 Genomic DNA. Translation: EAL66425.1 .
    RefSeqi XP_640403.1. XM_635311.1.

    3D structure databases

    ProteinModelPortali Q54T48.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0205083.

    Protein family/group databases

    MEROPSi C12.A11.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0304593 ; DDB0304593 ; DDB_G0282007 .
    GeneIDi 8623358.
    KEGGi ddi:DDB_G0282007.

    Organism-specific databases

    dictyBasei DDB_G0282007. uch1.

    Phylogenomic databases

    eggNOGi NOG327708.
    KOi K05609.
    OMAi KEMEVAH.
    PhylomeDBi Q54T48.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiUCHL_DICDI
    AccessioniPrimary (citable) accession number: Q54T48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3