Q54T48 (UCHL_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase
EC=3.4.19.12

Alternative name(s):
Ubiquitin thioesterase

Gene names

Name:	uch1
Synonyms:	uchl
ORF Names:	DDB_G0282007

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or nedd8 By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase C12 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Probable ubiquitin carboxyl-terminal hydrolase		PRO_0000331128
Regions
	Region	16 – 21	6	Interaction with ubiquitin By similarity
	Region	227 – 232	6	Interaction with ubiquitin By similarity
Sites
	Active site	103	1	Nucleophile By similarity
	Active site	177	1	Proton donor By similarity
	Site	192	1	Important for enzyme activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54T48 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: A0B7E6B2540C24C3	FASTA	255	29,106
	10 20 30 40 50 60 MSVDQELIET QKNWIPLEAN PEVLTTFMQS LGVSKDWEFC DIYGIDEGLL EMVPSPCVAV 70 80 90 100 110 120 ILLFPITNEY EDKRYKLEKE IEEKGQVLSD KVYFMKQYIG NACGTIGVIH SVLNNANVIE 130 140 150 160 170 180 FNENGFFKQF LDKTTSLSTE ERAISLLKNS EIEKSHEISA LQGQSNVPQE DEPVVLHFVS 190 200 210 220 230 240 FVHVDGHLYE LDGRKPFAIN HGESSAETLL KDTANVLQKM IDEDPKEIRF NLMGLVKKPN 250 EESEEEEEKE KEETK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000044 Genomic DNA. Translation: EAL66425.1.
RefSeq	XP_640403.1. XM_635311.1.
3D structure databases
HSSP	HSSP built from PDB template 1UCH based on UniProtKB P15374.
ProteinModelPortal	Q54T48.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0205083.
Protein family/group databases
MEROPS	C12.A11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0304593; DDB0304593; DDB_G0282007.
GeneID	8623358.
KEGG	ddi:DDB_G0282007.
Organism-specific databases
dictyBase	DDB_G0282007. uch1.
Phylogenomic databases
eggNOG	NOG327708.
KO	K05609.
OMA	QTEAPNI.
ProtClustDB	CLSZ2729025.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PRINTS	PR00707. UBCTHYDRLASE.
ProtoNet	Search...

Entry information

Entry name

UCHL_DICDI

Accession

Primary (citable) accession number: Q54T48

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)