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Q54T48 (UCHL_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ubiquitin carboxyl-terminal hydrolase

EC=3.4.19.12
Alternative name(s):
Ubiquitin thioesterase
Gene names
Name:uch1
Synonyms:uchl
ORF Names:DDB_G0282007
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or nedd8 By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the peptidase C12 family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionubiquitin-specific protease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255Probable ubiquitin carboxyl-terminal hydrolase
PRO_0000331128

Regions

Region16 – 216Interaction with ubiquitin By similarity
Region227 – 2326Interaction with ubiquitin By similarity

Sites

Active site1031Nucleophile By similarity
Active site1771Proton donor By similarity
Site1921Important for enzyme activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54T48 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: A0B7E6B2540C24C3

FASTA25529,106
        10         20         30         40         50         60 
MSVDQELIET QKNWIPLEAN PEVLTTFMQS LGVSKDWEFC DIYGIDEGLL EMVPSPCVAV 

        70         80         90        100        110        120 
ILLFPITNEY EDKRYKLEKE IEEKGQVLSD KVYFMKQYIG NACGTIGVIH SVLNNANVIE 

       130        140        150        160        170        180 
FNENGFFKQF LDKTTSLSTE ERAISLLKNS EIEKSHEISA LQGQSNVPQE DEPVVLHFVS 

       190        200        210        220        230        240 
FVHVDGHLYE LDGRKPFAIN HGESSAETLL KDTANVLQKM IDEDPKEIRF NLMGLVKKPN 

       250 
EESEEEEEKE KEETK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000044 Genomic DNA. Translation: EAL66425.1.
RefSeqXP_640403.1. XM_635311.1.

3D structure databases

ProteinModelPortalQ54T48.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0205083.

Protein family/group databases

MEROPSC12.A11.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0304593; DDB0304593; DDB_G0282007.
GeneID8623358.
KEGGddi:DDB_G0282007.

Organism-specific databases

dictyBaseDDB_G0282007. uch1.

Phylogenomic databases

eggNOGNOG327708.
OMAKEMEVAH.
PhylomeDBQ54T48.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSPR00707. UBCTHYDRLASE.
ProtoNetSearch...

Entry information

Entry nameUCHL_DICDI
AccessionPrimary (citable) accession number: Q54T48
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase