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Q54T48

- UCHL_DICDI

UniProt

Q54T48 - UCHL_DICDI

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Protein
Probable ubiquitin carboxyl-terminal hydrolase
Gene
uch1, uchl, DDB_G0282007
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or nedd8 By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Nucleophile By similarity
Active sitei177 – 1771Proton donor By similarity
Sitei192 – 1921Important for enzyme activity By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.A11.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase (EC:3.4.19.12)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:uch1
Synonyms:uchl
ORF Names:DDB_G0282007
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282007. uch1.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Probable ubiquitin carboxyl-terminal hydrolase
PRO_0000331128Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0205083.

Structurei

3D structure databases

ProteinModelPortaliQ54T48.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 216Interaction with ubiquitin By similarity
Regioni227 – 2326Interaction with ubiquitin By similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.

Phylogenomic databases

eggNOGiNOG327708.
KOiK05609.
OMAiKEMEVAH.
PhylomeDBiQ54T48.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

Q54T48-1 [UniParc]FASTAAdd to Basket

« Hide

MSVDQELIET QKNWIPLEAN PEVLTTFMQS LGVSKDWEFC DIYGIDEGLL    50
EMVPSPCVAV ILLFPITNEY EDKRYKLEKE IEEKGQVLSD KVYFMKQYIG 100
NACGTIGVIH SVLNNANVIE FNENGFFKQF LDKTTSLSTE ERAISLLKNS 150
EIEKSHEISA LQGQSNVPQE DEPVVLHFVS FVHVDGHLYE LDGRKPFAIN 200
HGESSAETLL KDTANVLQKM IDEDPKEIRF NLMGLVKKPN EESEEEEEKE 250
KEETK 255
Length:255
Mass (Da):29,106
Last modified:May 24, 2005 - v1
Checksum:iA0B7E6B2540C24C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000044 Genomic DNA. Translation: EAL66425.1.
RefSeqiXP_640403.1. XM_635311.1.

Genome annotation databases

EnsemblProtistsiDDB0304593; DDB0304593; DDB_G0282007.
GeneIDi8623358.
KEGGiddi:DDB_G0282007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000044 Genomic DNA. Translation: EAL66425.1 .
RefSeqi XP_640403.1. XM_635311.1.

3D structure databases

ProteinModelPortali Q54T48.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0205083.

Protein family/group databases

MEROPSi C12.A11.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0304593 ; DDB0304593 ; DDB_G0282007 .
GeneIDi 8623358.
KEGGi ddi:DDB_G0282007.

Organism-specific databases

dictyBasei DDB_G0282007. uch1.

Phylogenomic databases

eggNOGi NOG327708.
KOi K05609.
OMAi KEMEVAH.
PhylomeDBi Q54T48.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PRINTSi PR00707. UBCTHYDRLASE.
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiUCHL_DICDI
AccessioniPrimary (citable) accession number: Q54T48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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