Reviewed, UniProtKB/Swiss-Prot Q54SP4 (DHKD_DICDI)

Names and origin

Protein names

Recommended name:
    Hybrid signal transduction histidine kinase D
    EC=2.7.13.3

Gene names

Name:	dhkD
ORF Names:	DDB_G0282289

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1546 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts as a receptor histidine kinase for a signal transduction pathway. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Domain	Atypical domain architecture: contains 2 histidine kinase/receiver domain modules.
Sequence similarities	Contains 2 histidine kinase domains. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain. Contains 2 response regulatory domains.
Sequence caution	The sequence AAB84171.1 differs from that shown. Reason: Frameshift at position 703.

Ontologies

Keywords
Biological process	Two-component regulatory system
Domain	Coiled coil Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transducer Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1546	1546	Hybrid signal transduction histidine kinase D		PRO_0000328270
Regions
	Domain	65 – 131	67	PAS
	Domain	139 – 193	55	PAC
	Domain	218 – 440	223	Histidine kinase 1
	Domain	571 – 686	116	Response regulatory 1
	Domain	747 – 1010	264	Histidine kinase 2
	Domain	1359 – 1483	125	Response regulatory 2
	Coiled coil	36 – 63	28	Potential
	Compositional bias	29 – 32	4	Poly-Gln
	Compositional bias	465 – 479	15	Poly-Gln
	Compositional bias	499 – 512	14	Poly-Asp
	Compositional bias	1012 – 1020	9	Poly-Ser
	Compositional bias	1045 – 1120	76	Poly-Asn
	Compositional bias	1123 – 1132	10	Poly-Asn
	Compositional bias	1136 – 1146	11	Poly-Asn
	Compositional bias	1201 – 1212	12	Poly-Asn
	Compositional bias	1240 – 1250	11	Poly-Asn
	Compositional bias	1322 – 1337	16	Poly-Asn
	Compositional bias	1501 – 1527	27	Poly-Asn
Amino acid modifications
	Modified residue	221	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	619	1	4-aspartylphosphate By similarity
	Modified residue	750	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	1412	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	114 – 115	2	RW → SR in AAK50005. Ref.1
	Sequence conflict	114 – 115	2	RW → SR in AAB84171. Ref.3
	Sequence conflict	147	1	G → C in AAB84171. Ref.3
	Sequence conflict	292	1	V → A in AAB84171. Ref.3
	Sequence conflict	349	1	V → G in AAB84171. Ref.3
	Sequence conflict	471	1	Missing in AAB84171. Ref.3
	Sequence conflict	520	1	K → R in AAB84171. Ref.3
	Sequence conflict	1150	1	G → R in AAK50005. Ref.1
	Sequence conflict	1158	1	N → T in AAK50005. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54SP4-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 095D000FE94C245E	FASTA	1,546	172,908
	10 20 30 40 50 60 MQDISTTTAS TNYLKADGDT GAVFSITSQQ QQIHYMRKQK PDHEKTREEL IEEINHLRAV 70 80 90 100 110 120 SNSNKNARIM LDEMYQFVAL LDVNGNLLDV NEPALQGGGM IRSSIQGIPF WDCRWWATSQ 130 140 150 160 170 180 ENIDNVREAV HKASKGEFIR YETEIFGKSA GTEKITIDFS LMPLFNDKGE VSLILPEGRN 190 200 210 220 230 240 ITEKRLGELE IERKNNELRS LYEKIKELDE LKTQFFANVS HELRTPLALI VGPTDKLLKD 250 260 270 280 290 300 ENVDINVRKD LEIVARNARG LLKIVNNLLD ISRLEAGKMN LNYSMVNLGQ TVHLIASCFE 310 320 330 340 350 360 ILAREKSLDF SIITPSEPMM AAIDADKMQR VITNLISNAF KFTPSGGAVK CILEKFDLSP 370 380 390 400 410 420 NKPGFQIVVS DTGPGIPDNL HEIIFERFRQ VDGSSTRKHG GTGLGLSIVK EFVTLHGGTV 430 440 450 460 470 480 TIHNISTGGA QFTLRLPLTP NMDEHLYKKT ISDQNISEIQ QQLYQQQQQE QQQQQQQQQN 490 500 510 520 530 540 LQQPKLILNN IDNKYKINDD DNDDDDDDDD EDGLNFNILK KTNTSSDIAS NALKDNMGSI 550 560 570 580 590 600 MGVHAIAQQA VEELTEKQFY QSQENIHNKP IVLVVEDNPE MNRFIAELLS KYYFVVTAFD 610 620 630 640 650 660 GVEGIEKTRA ITPDLIVTDC MMPRMSGDEM VEQLRSDEQF DNIPILLLTA KADENLRIKL 670 680 690 700 710 720 LQNGVSDYVN KPFSSEELVA RVVNLMTMKK AKQFLQEELS SANTDLQELI NQLTNKKRDL 730 740 750 760 770 780 QTLVGELEKE RNLLNNTNKS KDEFFMNLSH ELRTPLNGIL GWCQLLLYDI DSGGSSGGGS 790 800 810 820 830 840 GSISGDDSTV RTGLETIERC ASSQNQLIND LLDMSLIIGD KFSLILGDVD LPILIENAIS 850 860 870 880 890 900 SILVTAQSKK ISVHSNIQGE GEEESSILRN IVGDKARLQQ VIWNLLSNSI KFTKEKGRIH 910 920 930 940 950 960 LNLKVVNQIP SREVLGFCSN PMFNTINRHG DRWILFTITD NGKGIPKQFL PSVFDRFKQA 970 980 990 1000 1010 1020 DCSSTRSYGG LGIGLSITQN IIHLHKGAVY ANSEGENKGS QFTVVLPIIK GSSSSNSSPS 1030 1040 1050 1060 1070 1080 NQLSCSSSPP LIKKPFFSQP TNYINNNNNN NNNNNNNNNN NNNNNNNNNN NNNYNNYNNN 1090 1100 1110 1120 1130 1140 NSNNNNNSNN NNNNNNNNNN NNNNNNNNNN NNNNNLNINN LINNNHNNNN NNYHHNNNNN 1150 1160 1170 1180 1190 1200 NNSNNNEKLG KRQREEINNN ENNNVDLRLR TPPPPPTSSI SENFLVNSSN LIPSPALNPI 1210 1220 1230 1240 1250 1260 NNNNNSNNNN NNIGDIKKIN LEISQIPSTN LNPKLSLVIN NNNNNNNNNN SLLKTIITNA 1270 1280 1290 1300 1310 1320 INNNINNSNN DDNSNNTTIP TPPSSAINMI NSLPLPSPSP SFILPTLSPF SSPLSELKSS 1330 1340 1350 1360 1370 1380 SNNNNNNNNN SNNNNNNSMS PNLRSPKANS NKNLLGGIQI MLVDDLEETT HLFSSMLYKL 1390 1400 1410 1420 1430 1440 GAKRIDTFQR VSDAYAFLCD SSKPQPDIIL SDLTMPFEDG YSMVRKLRDR EKINTQNKKT 1450 1460 1470 1480 1490 1500 PIIALTASVS SSDKEKVLKS GFDLHCSKPV NFLELSNSIL TLIEKYNTTV NIDLIKEQEQ 1510 1520 1530 1540 NNNNNNNNNN NNNNNNNNNN NNINNGNDDD SLLLTDSRPC KKANSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The histidine kinases of Dictyostelium." Anjard C., Loomis W.F. (In) Inouye M., Dutta R. (eds.); Histidine kinases in signal transduction, pp.1-1, Academic press, San Diego (2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"dhkD, a histidine kinase gene of Dictyostelium discoideum." Singleton C.K., Zinda M. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-744.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF361475 Genomic DNA. Translation: AAK50005.1. AAFI02000047 Genomic DNA. Translation: EAL66266.1. AF029704 mRNA. Translation: AAB84171.1. Frameshift.
RefSeq	XP_640257.1.
3D structure databases
HSSP	HSSP built from PDB template 1YSR based on UniProtKB P0A5Z8.
ModBase	Search...
Genome annotation databases
GeneID	8623516.
KEGG	ddi:DDB_0215384.
Organism-specific databases
dictyBase	DDB_G0282289. dhkD.
Phylogenomic databases
eggNOG	KOG0519.
PhylomeDB	Q54SP4.
Enzyme and pathway databases
BRENDA	2.7.13.3. 424.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011006. CheY-like. IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 2 hits.
Pfam	PF02518. HATPase_c. 2 hits. PF00512. HisKA. 2 hits. PF00072. Response_reg. 2 hits. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 2 hits. SM00388. HisKA. 2 hits. SM00086. PAC. 1 hit. SM00091. PAS. 1 hit. SM00448. REC. 2 hits. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS50109. HIS_KIN. 2 hits. PS50113. PAC. 1 hit. PS50112. PAS. False negative. PS50110. RESPONSE_REGULATORY. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHKD_DICDI

Accession

Primary (citable) accession number: Q54SP4
Secondary accession number(s): O15783, Q95PI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)