Reviewed, UniProtKB/Swiss-Prot Q54SD5 (PEM2_DICDI)

Names and origin

Protein names

Recommended name:
    Probable methylene-fatty-acyl-phospholipid synthase
    EC=2.1.1.16

Gene names

Name:	pem2
ORF Names:	DDB_G0282527

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid methylene fatty acid.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the PEMT/PEM2 methyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-methyltransferase activity Inferred from electronic annotation. Source: InterPro methylene-fatty-acyl-phospholipid synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Probable methylene-fatty-acyl-phospholipid synthase		PRO_0000328081
Regions
	Transmembrane	22 – 42	21	Potential
	Transmembrane	65 – 85	21	Potential
	Transmembrane	98 – 118	21	Potential
	Transmembrane	141 – 161	21	Potential
	Transmembrane	168 – 188	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54SD5-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 826826A1A1B55E0A	FASTA	213	24,515
	10 20 30 40 50 60 MIVEHAIDYI DYLMNYVDFT EKYFLLTIAC VVFNPTWWNI TARMEYKTKF MTKICGSKEN 70 80 90 100 110 120 GCYLLAFLIF SLGILRDWLF SEALIRQPIF QEFDRFEVEV LSYILYGFGG ILVLAAYLKL 130 140 150 160 170 180 GITGTYLGDY FGILMKERVT GFPFNVMNNP MYNGSVMLFI AHALSYKSVA GLVLSFVVYV 190 200 210 VYKFALIFEE SFTNYIYSTA AANAAKKNKS KSK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000047 Genomic DNA. Translation: EAL66123.1.
RefSeq	XP_640104.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3391184.
KEGG	ddi:DDBDRAFT_0204815.
Organism-specific databases
dictyBase	DDB_G0282527. pem2.
Phylogenomic databases
OMA	Q54SD5. VARWEHK.
Enzyme and pathway databases
BRENDA	2.1.1.16. 424.
Family and domain databases
InterPro	IPR007318. PEMT. [Graphical view]
PANTHER	PTHR15458. PEMT. 1 hit.
Pfam	PF04191. PEMT. 1 hit. [Graphical view]
PIRSF	PIRSF005444. PEMT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PEM2_DICDI

Accession

Primary (citable) accession number: Q54SD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)