Reviewed, UniProtKB/Swiss-Prot Q54SC9 (HEXA2_DICDI)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase subunit A2
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase subunit A2
    Beta-N-acetylhexosaminidase subunit A2

Gene names

Name:	hexa2
ORF Names:	DDB_G0282539

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 541	520	Beta-hexosaminidase subunit A2		PRO_0000331236
Sites
	Active site	314	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54SC9-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: F670234F1DD3D075	FASTA	541	61,533
	10 20 30 40 50 60 MINKFLTIFL IFSIVIIKVL SQSSNEQPLN VVPYPQEVTM IGCNIPLSVG SISIKSNIES 70 80 90 100 110 120 TILSISISRY QSLFFPFVSN NVLKDSSSNI ELSLIIASDD ETLELGIDES YFLLVNQDTY 130 140 150 160 170 180 QIKANTIYGA MRGLETFKQM VVYDVVENSY SLTCAEVVDY PTYQWRGLLV DNARHFLPKN 190 200 210 220 230 240 MVLHIIDSMG YNKFNTMHWH LIDTVAFPVE SKTYPKLTEA LLGPGAIITH DDILEVVAYA 250 260 270 280 290 300 KTYGIRVIPE FDVPGHSASW GVGYPELLSN CPGYPQSSIP LDCSNPYTYS FLENFFSEIA 310 320 330 340 350 360 PLFQDSYFHT GGDELVIDCW ANDTSIQKWM KTNNYNTSDA FQYFEDQLDV ILKSINRTKI 370 380 390 400 410 420 AWNDVLQHGV KFDKETTLVQ TWTNINDLRD VLAAGYKTIT SFFFYLDRQS PTGNHYHYEW 430 440 450 460 470 480 QDTWEDFYAS DPRLNITSNA ENILGGEATM FGEQVSTVNW DARVWPRAIG ISERLWSATE 490 500 510 520 530 540 INNITLALPR IGQFSCDMSR RGISSGPLFP DFCSLPDDLS FSFKPVYQLS KDEIKLILKK K

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000047 Genomic DNA. Translation: EAL66129.1.
RefSeq	XP_640110.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54SC9.
Genome annotation databases
GeneID	3391190.
GenomeReviews	Gene locus hexa2 in contig CM000152_GR.
KEGG	ddi:DDBDRAFT_0204822.
Organism-specific databases
dictyBase	DDB_G0282539. hexa2.
Phylogenomic databases
OMA	NIFCAGK.
Family and domain databases
InterPro	IPR001540. Glyco_hydro_20. IPR015883. Glyco_hydro_20_cat-core. IPR013781. Glyco_hydro_sg_catalytic. IPR015882. HexNAc-like_b. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR22600. Glyco_hydro_20. 1 hit.
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PRINTS	PR00738. GLHYDRLASE20.
ProtoNet	Search...

Entry information

Entry name

HEXA2_DICDI

Accession

Primary (citable) accession number: Q54SC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)