Reviewed, UniProtKB/Swiss-Prot Q54SA1 (PLDZ_DICDI)

Names and origin

Protein names

Recommended name:
    Phospholipase D Z
    EC=3.1.4.4
Alternative name(s):
    Phosphatase D3
      Short name=PLD 3

Gene names

Name:	pldZ
Synonyms:	pld3
ORF Names:	DDB_G0282579

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes membrane phospholipids, such as PtdCho (phosphatidylcholine), producing the free headgroup and PtdOH (phosphatidic acid; signaling molecule on its own) By similarity.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Enzyme regulation	Inhibited by butan-1-ol.
Sequence similarities	Belongs to the phospholipase D family. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 422	404	Phospholipase D Z		PRO_0000367474
Regions
	Domain	148 – 175	28	PLD phosphodiesterase 1
	Domain	357 – 383	27	PLD phosphodiesterase 2
Sites
	Active site	153	1	Potential
	Active site	155	1	Potential
	Active site	160	1	Potential
	Active site	362	1	Potential
	Active site	364	1	Potential
	Active site	369	1	Potential
Amino acid modifications
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Glycosylation	320	1	N-linked (GlcNAc...) Potential
	Glycosylation	378	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54SA1-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 844CFF1EE8CAA356	FASTA	422	47,101
	10 20 30 40 50 60 MMMKLLFLIA LFGCVVNSIR INNDNGLSFQ SNCLGGSIQI AESIPLALNL QSNLSTHDAW 70 80 90 100 110 120 MELITNAKKS IDMGIFYMTL TDGGQLDPVY GGQLGLDIYK ALVDANSRGV SIRIVQNQPS 130 140 150 160 170 180 SSMPDTDTQN LAKLGVQVRS INWPSLVGAG ILHTKVIVVD QVSAYLGSAN LDWRSLAQVK 190 200 210 220 230 240 ELGVLFQNCP SMVSDTEIAF QQYWDAAVVT ELPSDWGVQY QAAYNQTNMA SLLLNGNEKF 250 260 270 280 290 300 EMFLAVSPPQ FVSTDRTGDI DALVSAMNGA TKTICISVMD YIPASLYNSP NTFWPVMDNA 310 320 330 340 350 360 LRAAAYNRGV QVRMLISHWN HTNYAIPQWL HSLDQVNNID VRWFVVPDFP NEPQVPFTRV 370 380 390 400 410 420 NHAKYMVTDE QSYVGTSNWS EDYYTNTGGL SYNIYNDEFT SQLQSIFDRD WNSPYSFPVT TY

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000047 Genomic DNA. Translation: EAL66149.1.
RefSeq	XP_640138.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3391210.
GenomeReviews	Gene locus pldZ in contig CM000152_GR.
KEGG	ddi:DDB_0231505.
Organism-specific databases
dictyBase	DDB_G0282579. pldZ.
Phylogenomic databases
OMA	VFERDWN.
Family and domain databases
InterPro	IPR013582. PLD_envelope. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
Pfam	PF08371. PLD_envelope. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDZ_DICDI

Accession

Primary (citable) accession number: Q54SA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)