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Protein

Cyclic GMP-binding protein D

Gene

gbpD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP (By similarity). Induces the formation of substrate-attached pseudopodia, that leads to increased adhesion and thereby negatively influencing cell speed and polarity.By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi698 – 857cNMP 1Add BLAST160
Nucleotide bindingi1105 – 1218cNMP 2Add BLAST114
Nucleotide bindingi1182 – 1303cNMP 3Add BLAST122

GO - Molecular functioni

  • nucleotide binding Source: UniProtKB-KW
  • Rap guanyl-nucleotide exchange factor activity Source: dictyBase

GO - Biological processi

  • cell-substrate adhesion Source: dictyBase
  • cGMP-mediated signaling Source: dictyBase
  • establishment or maintenance of cell polarity Source: dictyBase
  • positive regulation of cellular component movement Source: dictyBase
  • regulation of chemotaxis Source: dictyBase
  • regulation of lateral pseudopodium assembly Source: dictyBase
  • small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandcGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-354192 Integrin alphaIIb beta3 signaling
R-DDI-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-DDI-392517 Rap1 signalling
R-DDI-5673001 RAF/MAP kinase cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic GMP-binding protein D
Alternative name(s):
Guanine nucleotide exchange factor for rap1
Ras guanine nucleotide exchange factor U
RasGEF domain-containing protein U
Gene namesi
Name:gbpD
Synonyms:gefU, rapgef
ORF Names:DDB_G0282373
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282373 gbpD

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003531041 – 1312Cyclic GMP-binding protein DAdd BLAST1312

Proteomic databases

PaxDbiQ54S40
PRIDEiQ54S40

Expressioni

Developmental stagei

Increases after starvation is initiated with a peak between 8-12 hours of starvation.2 Publications

Interactioni

GO - Molecular functioni

  • Rap guanyl-nucleotide exchange factor activity Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0215332

Structurei

3D structure databases

ProteinModelPortaliQ54S40
SMRiQ54S40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 155N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST130
Domaini353 – 582Ras-GEFPROSITE-ProRule annotationAdd BLAST230
Domaini940 – 1006GRAMAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi216 – 227Poly-AsnAdd BLAST12
Compositional biasi267 – 272Poly-Gly6
Compositional biasi311 – 326Poly-AsnAdd BLAST16
Compositional biasi604 – 607Poly-Ser4
Compositional biasi613 – 624Poly-GlyAdd BLAST12
Compositional biasi666 – 670Poly-Ser5
Compositional biasi759 – 766Poly-Gly8
Compositional biasi893 – 898Poly-Gly6
Compositional biasi1047 – 1062Poly-GlnAdd BLAST16
Compositional biasi1167 – 1201Poly-AsnAdd BLAST35

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3417 Eukaryota
ENOG410XR96 LUCA
InParanoidiQ54S40
OMAiNRISSWV

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd06224 REM, 1 hit
Gene3Di1.10.840.10, 1 hit
2.30.29.30, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR004182 GRAM
IPR011993 PH-like_dom_sf
IPR000651 Ras-like_Gua-exchang_fac_N
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF02893 GRAM, 1 hit
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
SMARTiView protein in SMART
SM00568 GRAM, 1 hit
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 1 hit
SUPFAMiSSF48366 SSF48366, 2 hits
SSF51206 SSF51206, 4 hits
PROSITEiView protein in PROSITE
PS50042 CNMP_BINDING_3, 2 hits
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q54S40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYPFYKPD DETNIIYNIN INKKEGFSAI KSCSLAKLIE KLTSTKQLHT
60 70 80 90 100
YFSSAFFLTY RDFTTPLEII NLLISRYTGP PPDSSKDSLR LFEIEVDIVQ
110 120 130 140 150
ANVLSIFRTL IGTLIQVDFD TPKLSSSIIE FFNSLPESVK NELFLEFFKA
160 170 180 190 200
KKMARPPGSI PKPVNSNIIS SAASTMRFSY SVSPKTQSPN STNNVLSGLL
210 220 230 240 250
SNNGSNTMNG INGTSNNNVN SNNNNNNGTT NISSPNFDPS RSSMKMGKGI
260 270 280 290 300
MKLLQSNIAN FNNQQNGGGG GGSNNGTSPQ SSPSSTLSST NSLFNQQPSL
310 320 330 340 350
SMLNDDGSVQ NNNNNNNNNN NNNNVNEIST INVQLPPSTP PINGIPGEKT
360 370 380 390 400
AFLPEAIAKE LTIMEWELIT ALSVSDITSK EWNKSVNIQN LTTWFNRISS
410 420 430 440 450
WVSTKIISKE TPEERAIIIE AFINIANFAK ELKNYNCVME ILGSLHGSSI
460 470 480 490 500
SRLKSSWALL SQKTNDMFQI LNNLMTTDGN FKNYRKILTT VLPNEPCIPY
510 520 530 540 550
LGLFLTDYTY LDESNPSLST DSNLINIDRI FLISTRVQEF FQLFTNCNYT
560 570 580 590 600
FISNMSVRDA ILGEKVWDEN EIFRLSKIRE ETSSLQSLKE SNGIGNSNST
610 620 630 640 650
SGGSSSSLVN KDGSGGGGGS GGGGSVNSKG DGKGDGKDNR DGRGDGNSNC
660 670 680 690 700
GNGSGISSKD KDLLLSSSSS AASHTVRRKN FVTKYRMSFT GNDPLPSVSS
710 720 730 740 750
TLSEREWKIL TTNSKTIIYP RGKTVLSVGE TNTNIYRVIS GRVKVETLKS
760 770 780 790 800
FNSSDADLGA GGGGGGDNRG EDNSLMAIKA RNRLSLNLPA QDDGYYVEEG
810 820 830 840 850
EIFGQESFLY TDRPMLSNII VDSGECELME IEKSFVLQLF ASEHQLSATF
860 870 880 890 900
YKFIGVIQAQ LLKSIYINFT SNYGNGGSGG SGSNGNGGGS SNGAGGGGLS
910 920 930 940 950
SSVSIGNLNS PNINRIDFKR RSTIFETPSS LDILRDGNDS SFRTKFGLSQ
960 970 980 990 1000
DEVIIKRYQC KHNNMNGTLY ITKHNLCFEG KFLGINKNKT IPFDRTDKIL
1010 1020 1030 1040 1050
TVDKNIMTVT TKEKVKKFTF KSHDDLNEGY GISVQIWANH LNINVKQQLQ
1060 1070 1080 1090 1100
LQQQQLQQQQ QQPSQQPSQQ QSQSSQLQQS VSASSTTPPV PNSPKVGRGE
1110 1120 1130 1140 1150
SFSNIKDLPS KDEWNQILKG TKPLTFKKGE ILICEGVEYQ KMFQIVKGEC
1160 1170 1180 1190 1200
SVVKSLLPTP LDQNIFNNIN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN
1210 1220 1230 1240 1250
NSIFKNNSSI SNTNSNTSID GGVGGGLSPQ TLVDPKNSVI VSKLTHGSIF
1260 1270 1280 1290 1300
GEMSFLLPGG SATSLVVSSD EVEVYIIESY FLHILLKSKP YLASKFYKYL
1310
ACVLETRVRN LT
Length:1,312
Mass (Da):143,659
Last modified:May 24, 2005 - v1
Checksum:i044A4E51DBA2192E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti621G → A in AAM34042 (PubMed:12011437).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF481924 Genomic DNA Translation: AAM34042.1
AAFI02000047 Genomic DNA Translation: EAL66046.1
RefSeqiXP_640199.1, XM_635107.1

Genome annotation databases

EnsemblProtistsiEAL66046; EAL66046; DDB_G0282373
GeneIDi8623739
KEGGiddi:DDB_G0282373

Entry informationi

Entry nameiGBPD_DICDI
AccessioniPrimary (citable) accession number: Q54S40
Secondary accession number(s): Q8MVR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 24, 2005
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

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