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Q54S40

- GBPD_DICDI

UniProt

Q54S40 - GBPD_DICDI

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Protein
Cyclic GMP-binding protein D
Gene
gbpD, gefU, rapgef, DDB_G0282373
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP By similarity. Induces the formation of substrate-attached pseudopodia, that leads to increased adhesion and thereby negatively influencing cell speed and polarity.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi698 – 857160cNMP 1
Add
BLAST
Nucleotide bindingi1105 – 1218114cNMP 2
Add
BLAST
Nucleotide bindingi1182 – 1303122cNMP 3
Add
BLAST

GO - Molecular functioni

  1. Rap guanyl-nucleotide exchange factor activity Source: dictyBase
  2. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. cell-substrate adhesion Source: dictyBase
  2. establishment or maintenance of cell polarity Source: dictyBase
  3. positive regulation of cellular component movement Source: dictyBase
  4. regulation of Rap GTPase activity Source: GOC
  5. regulation of chemotaxis Source: dictyBase
  6. regulation of lateral pseudopodium assembly Source: dictyBase
  7. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic GMP-binding protein D
Alternative name(s):
Guanine nucleotide exchange factor for rap1
Ras guanine nucleotide exchange factor U
RasGEF domain-containing protein U
Gene namesi
Name:gbpD
Synonyms:gefU, rapgef
ORF Names:DDB_G0282373
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282373. gbpD.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13121312Cyclic GMP-binding protein D
PRO_0000353104Add
BLAST

Expressioni

Developmental stagei

Increases after starvation is initiated with a peak between 8-12 hours of starvation.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0215332.

Structurei

3D structure databases

ProteinModelPortaliQ54S40.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 155130N-terminal Ras-GEF
Add
BLAST
Domaini353 – 582230Ras-GEF
Add
BLAST
Domaini940 – 100667GRAM
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi216 – 22712Poly-Asn
Add
BLAST
Compositional biasi267 – 2726Poly-Gly
Compositional biasi311 – 32616Poly-Asn
Add
BLAST
Compositional biasi604 – 6074Poly-Ser
Compositional biasi613 – 62412Poly-Gly
Add
BLAST
Compositional biasi666 – 6705Poly-Ser
Compositional biasi759 – 7668Poly-Gly
Compositional biasi893 – 8986Poly-Gly
Compositional biasi1047 – 106216Poly-Gln
Add
BLAST
Compositional biasi1167 – 120135Poly-Asn
Add
BLAST

Sequence similaritiesi

Contains 1 GRAM domain.
Contains 1 Ras-GEF domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3417.
OMAiEAFINIA.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
2.60.120.10. 3 hits.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR004182. GRAM.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF02893. GRAM. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF51206. SSF51206. 4 hits.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54S40-1 [UniParc]FASTAAdd to Basket

« Hide

MTDYPFYKPD DETNIIYNIN INKKEGFSAI KSCSLAKLIE KLTSTKQLHT     50
YFSSAFFLTY RDFTTPLEII NLLISRYTGP PPDSSKDSLR LFEIEVDIVQ 100
ANVLSIFRTL IGTLIQVDFD TPKLSSSIIE FFNSLPESVK NELFLEFFKA 150
KKMARPPGSI PKPVNSNIIS SAASTMRFSY SVSPKTQSPN STNNVLSGLL 200
SNNGSNTMNG INGTSNNNVN SNNNNNNGTT NISSPNFDPS RSSMKMGKGI 250
MKLLQSNIAN FNNQQNGGGG GGSNNGTSPQ SSPSSTLSST NSLFNQQPSL 300
SMLNDDGSVQ NNNNNNNNNN NNNNVNEIST INVQLPPSTP PINGIPGEKT 350
AFLPEAIAKE LTIMEWELIT ALSVSDITSK EWNKSVNIQN LTTWFNRISS 400
WVSTKIISKE TPEERAIIIE AFINIANFAK ELKNYNCVME ILGSLHGSSI 450
SRLKSSWALL SQKTNDMFQI LNNLMTTDGN FKNYRKILTT VLPNEPCIPY 500
LGLFLTDYTY LDESNPSLST DSNLINIDRI FLISTRVQEF FQLFTNCNYT 550
FISNMSVRDA ILGEKVWDEN EIFRLSKIRE ETSSLQSLKE SNGIGNSNST 600
SGGSSSSLVN KDGSGGGGGS GGGGSVNSKG DGKGDGKDNR DGRGDGNSNC 650
GNGSGISSKD KDLLLSSSSS AASHTVRRKN FVTKYRMSFT GNDPLPSVSS 700
TLSEREWKIL TTNSKTIIYP RGKTVLSVGE TNTNIYRVIS GRVKVETLKS 750
FNSSDADLGA GGGGGGDNRG EDNSLMAIKA RNRLSLNLPA QDDGYYVEEG 800
EIFGQESFLY TDRPMLSNII VDSGECELME IEKSFVLQLF ASEHQLSATF 850
YKFIGVIQAQ LLKSIYINFT SNYGNGGSGG SGSNGNGGGS SNGAGGGGLS 900
SSVSIGNLNS PNINRIDFKR RSTIFETPSS LDILRDGNDS SFRTKFGLSQ 950
DEVIIKRYQC KHNNMNGTLY ITKHNLCFEG KFLGINKNKT IPFDRTDKIL 1000
TVDKNIMTVT TKEKVKKFTF KSHDDLNEGY GISVQIWANH LNINVKQQLQ 1050
LQQQQLQQQQ QQPSQQPSQQ QSQSSQLQQS VSASSTTPPV PNSPKVGRGE 1100
SFSNIKDLPS KDEWNQILKG TKPLTFKKGE ILICEGVEYQ KMFQIVKGEC 1150
SVVKSLLPTP LDQNIFNNIN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN 1200
NSIFKNNSSI SNTNSNTSID GGVGGGLSPQ TLVDPKNSVI VSKLTHGSIF 1250
GEMSFLLPGG SATSLVVSSD EVEVYIIESY FLHILLKSKP YLASKFYKYL 1300
ACVLETRVRN LT 1312
Length:1,312
Mass (Da):143,659
Last modified:May 24, 2005 - v1
Checksum:i044A4E51DBA2192E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti621 – 6211G → A in AAM34042. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF481924 Genomic DNA. Translation: AAM34042.1.
AAFI02000047 Genomic DNA. Translation: EAL66046.1.
RefSeqiXP_640199.1. XM_635107.1.

Genome annotation databases

EnsemblProtistsiDDB0215332; DDB0215332; DDB_G0282373.
GeneIDi8623739.
KEGGiddi:DDB_G0282373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF481924 Genomic DNA. Translation: AAM34042.1 .
AAFI02000047 Genomic DNA. Translation: EAL66046.1 .
RefSeqi XP_640199.1. XM_635107.1.

3D structure databases

ProteinModelPortali Q54S40.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0215332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0215332 ; DDB0215332 ; DDB_G0282373 .
GeneIDi 8623739.
KEGGi ddi:DDB_G0282373.

Organism-specific databases

dictyBasei DDB_G0282373. gbpD.

Phylogenomic databases

eggNOGi KOG3417.
OMAi EAFINIA.

Family and domain databases

Gene3Di 1.10.840.10. 1 hit.
2.60.120.10. 3 hits.
InterProi IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR004182. GRAM.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF02893. GRAM. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view ]
SMARTi SM00568. GRAM. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view ]
SUPFAMi SSF48366. SSF48366. 2 hits.
SSF51206. SSF51206. 4 hits.
PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium."
    Bosgraaf L., Russcher H., Smith J.L., Wessels D., Soll D.R., Van Haastert P.J.M.
    EMBO J. 21:4560-4570(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: DEVELOPMENTAL STAGE.
  5. "RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium."
    Bosgraaf L., Waijer A., Engel R., Visser A.J.W.G., Wessels D., Soll D., van Haastert P.J.M.
    J. Cell Sci. 118:1899-1910(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGBPD_DICDI
AccessioniPrimary (citable) accession number: Q54S40
Secondary accession number(s): Q8MVR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3