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Q54S40

- GBPD_DICDI

UniProt

Q54S40 - GBPD_DICDI

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Protein

Cyclic GMP-binding protein D

Gene

gbpD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP (By similarity). Induces the formation of substrate-attached pseudopodia, that leads to increased adhesion and thereby negatively influencing cell speed and polarity.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi698 – 857160cNMP 1Add
BLAST
Nucleotide bindingi1105 – 1218114cNMP 2Add
BLAST
Nucleotide bindingi1182 – 1303122cNMP 3Add
BLAST

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. Rap guanyl-nucleotide exchange factor activity Source: dictyBase

GO - Biological processi

  1. cell-substrate adhesion Source: dictyBase
  2. establishment or maintenance of cell polarity Source: dictyBase
  3. positive regulation of cellular component movement Source: dictyBase
  4. positive regulation of Rap GTPase activity Source: GOC
  5. regulation of chemotaxis Source: dictyBase
  6. regulation of lateral pseudopodium assembly Source: dictyBase
  7. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic GMP-binding protein D
Alternative name(s):
Guanine nucleotide exchange factor for rap1
Ras guanine nucleotide exchange factor U
RasGEF domain-containing protein U
Gene namesi
Name:gbpD
Synonyms:gefU, rapgef
ORF Names:DDB_G0282373
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282373. gbpD.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13121312Cyclic GMP-binding protein DPRO_0000353104Add
BLAST

Expressioni

Developmental stagei

Increases after starvation is initiated with a peak between 8-12 hours of starvation.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0215332.

Structurei

3D structure databases

ProteinModelPortaliQ54S40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 155130N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini353 – 582230Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini940 – 100667GRAMAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi216 – 22712Poly-AsnAdd
BLAST
Compositional biasi267 – 2726Poly-Gly
Compositional biasi311 – 32616Poly-AsnAdd
BLAST
Compositional biasi604 – 6074Poly-Ser
Compositional biasi613 – 62412Poly-GlyAdd
BLAST
Compositional biasi666 – 6705Poly-Ser
Compositional biasi759 – 7668Poly-Gly
Compositional biasi893 – 8986Poly-Gly
Compositional biasi1047 – 106216Poly-GlnAdd
BLAST
Compositional biasi1167 – 120135Poly-AsnAdd
BLAST

Sequence similaritiesi

Contains 3 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 GRAM domain.Curated
Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3417.
InParanoidiQ54S40.
OMAiEAFINIA.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
2.60.120.10. 3 hits.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR004182. GRAM.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF02893. GRAM. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF51206. SSF51206. 4 hits.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54S40 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDYPFYKPD DETNIIYNIN INKKEGFSAI KSCSLAKLIE KLTSTKQLHT
60 70 80 90 100
YFSSAFFLTY RDFTTPLEII NLLISRYTGP PPDSSKDSLR LFEIEVDIVQ
110 120 130 140 150
ANVLSIFRTL IGTLIQVDFD TPKLSSSIIE FFNSLPESVK NELFLEFFKA
160 170 180 190 200
KKMARPPGSI PKPVNSNIIS SAASTMRFSY SVSPKTQSPN STNNVLSGLL
210 220 230 240 250
SNNGSNTMNG INGTSNNNVN SNNNNNNGTT NISSPNFDPS RSSMKMGKGI
260 270 280 290 300
MKLLQSNIAN FNNQQNGGGG GGSNNGTSPQ SSPSSTLSST NSLFNQQPSL
310 320 330 340 350
SMLNDDGSVQ NNNNNNNNNN NNNNVNEIST INVQLPPSTP PINGIPGEKT
360 370 380 390 400
AFLPEAIAKE LTIMEWELIT ALSVSDITSK EWNKSVNIQN LTTWFNRISS
410 420 430 440 450
WVSTKIISKE TPEERAIIIE AFINIANFAK ELKNYNCVME ILGSLHGSSI
460 470 480 490 500
SRLKSSWALL SQKTNDMFQI LNNLMTTDGN FKNYRKILTT VLPNEPCIPY
510 520 530 540 550
LGLFLTDYTY LDESNPSLST DSNLINIDRI FLISTRVQEF FQLFTNCNYT
560 570 580 590 600
FISNMSVRDA ILGEKVWDEN EIFRLSKIRE ETSSLQSLKE SNGIGNSNST
610 620 630 640 650
SGGSSSSLVN KDGSGGGGGS GGGGSVNSKG DGKGDGKDNR DGRGDGNSNC
660 670 680 690 700
GNGSGISSKD KDLLLSSSSS AASHTVRRKN FVTKYRMSFT GNDPLPSVSS
710 720 730 740 750
TLSEREWKIL TTNSKTIIYP RGKTVLSVGE TNTNIYRVIS GRVKVETLKS
760 770 780 790 800
FNSSDADLGA GGGGGGDNRG EDNSLMAIKA RNRLSLNLPA QDDGYYVEEG
810 820 830 840 850
EIFGQESFLY TDRPMLSNII VDSGECELME IEKSFVLQLF ASEHQLSATF
860 870 880 890 900
YKFIGVIQAQ LLKSIYINFT SNYGNGGSGG SGSNGNGGGS SNGAGGGGLS
910 920 930 940 950
SSVSIGNLNS PNINRIDFKR RSTIFETPSS LDILRDGNDS SFRTKFGLSQ
960 970 980 990 1000
DEVIIKRYQC KHNNMNGTLY ITKHNLCFEG KFLGINKNKT IPFDRTDKIL
1010 1020 1030 1040 1050
TVDKNIMTVT TKEKVKKFTF KSHDDLNEGY GISVQIWANH LNINVKQQLQ
1060 1070 1080 1090 1100
LQQQQLQQQQ QQPSQQPSQQ QSQSSQLQQS VSASSTTPPV PNSPKVGRGE
1110 1120 1130 1140 1150
SFSNIKDLPS KDEWNQILKG TKPLTFKKGE ILICEGVEYQ KMFQIVKGEC
1160 1170 1180 1190 1200
SVVKSLLPTP LDQNIFNNIN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN
1210 1220 1230 1240 1250
NSIFKNNSSI SNTNSNTSID GGVGGGLSPQ TLVDPKNSVI VSKLTHGSIF
1260 1270 1280 1290 1300
GEMSFLLPGG SATSLVVSSD EVEVYIIESY FLHILLKSKP YLASKFYKYL
1310
ACVLETRVRN LT
Length:1,312
Mass (Da):143,659
Last modified:May 24, 2005 - v1
Checksum:i044A4E51DBA2192E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti621 – 6211G → A in AAM34042. (PubMed:12011437)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF481924 Genomic DNA. Translation: AAM34042.1.
AAFI02000047 Genomic DNA. Translation: EAL66046.1.
RefSeqiXP_640199.1. XM_635107.1.

Genome annotation databases

EnsemblProtistsiDDB0215332; DDB0215332; DDB_G0282373.
GeneIDi8623739.
KEGGiddi:DDB_G0282373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF481924 Genomic DNA. Translation: AAM34042.1 .
AAFI02000047 Genomic DNA. Translation: EAL66046.1 .
RefSeqi XP_640199.1. XM_635107.1.

3D structure databases

ProteinModelPortali Q54S40.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0215332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0215332 ; DDB0215332 ; DDB_G0282373 .
GeneIDi 8623739.
KEGGi ddi:DDB_G0282373.

Organism-specific databases

dictyBasei DDB_G0282373. gbpD.

Phylogenomic databases

eggNOGi KOG3417.
InParanoidi Q54S40.
OMAi EAFINIA.

Family and domain databases

Gene3Di 1.10.840.10. 1 hit.
2.60.120.10. 3 hits.
InterProi IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR004182. GRAM.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF02893. GRAM. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view ]
SMARTi SM00568. GRAM. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view ]
SUPFAMi SSF48366. SSF48366. 2 hits.
SSF51206. SSF51206. 4 hits.
PROSITEi PS50042. CNMP_BINDING_3. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium."
    Bosgraaf L., Russcher H., Smith J.L., Wessels D., Soll D.R., Van Haastert P.J.M.
    EMBO J. 21:4560-4570(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: DEVELOPMENTAL STAGE.
  5. "RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium."
    Bosgraaf L., Waijer A., Engel R., Visser A.J.W.G., Wessels D., Soll D., van Haastert P.J.M.
    J. Cell Sci. 118:1899-1910(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGBPD_DICDI
AccessioniPrimary (citable) accession number: Q54S40
Secondary accession number(s): Q8MVR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3