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Q54S40 (GBPD_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclic GMP-binding protein D
Alternative name(s):
Guanine nucleotide exchange factor for rap1
Ras guanine nucleotide exchange factor U
RasGEF domain-containing protein U
Gene names
Name:gbpD
Synonyms:gefU, rapgef
ORF Names:DDB_G0282373
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1312 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Promotes the exchange of Ras-bound GDP by GTP By similarity. Induces the formation of substrate-attached pseudopodia, that leads to increased adhesion and thereby negatively influencing cell speed and polarity. Ref.3 Ref.5

Developmental stage

Increases after starvation is initiated with a peak between 8-12 hours of starvation. Ref.1 Ref.4

Sequence similarities

Contains 3 cyclic nucleotide-binding domains.

Contains 1 GRAM domain.

Contains 1 N-terminal Ras-GEF domain.

Contains 1 Ras-GEF domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13121312Cyclic GMP-binding protein D
PRO_0000353104

Regions

Domain26 – 155130N-terminal Ras-GEF
Domain353 – 582230Ras-GEF
Domain940 – 100667GRAM
Nucleotide binding698 – 857160cNMP 1
Nucleotide binding1105 – 1218114cNMP 2
Nucleotide binding1182 – 1303122cNMP 3
Compositional bias216 – 22712Poly-Asn
Compositional bias267 – 2726Poly-Gly
Compositional bias311 – 32616Poly-Asn
Compositional bias604 – 6074Poly-Ser
Compositional bias613 – 62412Poly-Gly
Compositional bias666 – 6705Poly-Ser
Compositional bias759 – 7668Poly-Gly
Compositional bias893 – 8986Poly-Gly
Compositional bias1047 – 106216Poly-Gln
Compositional bias1167 – 120135Poly-Asn

Experimental info

Sequence conflict6211G → A in AAM34042. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q54S40 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 044A4E51DBA2192E

FASTA1,312143,659
        10         20         30         40         50         60 
MTDYPFYKPD DETNIIYNIN INKKEGFSAI KSCSLAKLIE KLTSTKQLHT YFSSAFFLTY 

        70         80         90        100        110        120 
RDFTTPLEII NLLISRYTGP PPDSSKDSLR LFEIEVDIVQ ANVLSIFRTL IGTLIQVDFD 

       130        140        150        160        170        180 
TPKLSSSIIE FFNSLPESVK NELFLEFFKA KKMARPPGSI PKPVNSNIIS SAASTMRFSY 

       190        200        210        220        230        240 
SVSPKTQSPN STNNVLSGLL SNNGSNTMNG INGTSNNNVN SNNNNNNGTT NISSPNFDPS 

       250        260        270        280        290        300 
RSSMKMGKGI MKLLQSNIAN FNNQQNGGGG GGSNNGTSPQ SSPSSTLSST NSLFNQQPSL 

       310        320        330        340        350        360 
SMLNDDGSVQ NNNNNNNNNN NNNNVNEIST INVQLPPSTP PINGIPGEKT AFLPEAIAKE 

       370        380        390        400        410        420 
LTIMEWELIT ALSVSDITSK EWNKSVNIQN LTTWFNRISS WVSTKIISKE TPEERAIIIE 

       430        440        450        460        470        480 
AFINIANFAK ELKNYNCVME ILGSLHGSSI SRLKSSWALL SQKTNDMFQI LNNLMTTDGN 

       490        500        510        520        530        540 
FKNYRKILTT VLPNEPCIPY LGLFLTDYTY LDESNPSLST DSNLINIDRI FLISTRVQEF 

       550        560        570        580        590        600 
FQLFTNCNYT FISNMSVRDA ILGEKVWDEN EIFRLSKIRE ETSSLQSLKE SNGIGNSNST 

       610        620        630        640        650        660 
SGGSSSSLVN KDGSGGGGGS GGGGSVNSKG DGKGDGKDNR DGRGDGNSNC GNGSGISSKD 

       670        680        690        700        710        720 
KDLLLSSSSS AASHTVRRKN FVTKYRMSFT GNDPLPSVSS TLSEREWKIL TTNSKTIIYP 

       730        740        750        760        770        780 
RGKTVLSVGE TNTNIYRVIS GRVKVETLKS FNSSDADLGA GGGGGGDNRG EDNSLMAIKA 

       790        800        810        820        830        840 
RNRLSLNLPA QDDGYYVEEG EIFGQESFLY TDRPMLSNII VDSGECELME IEKSFVLQLF 

       850        860        870        880        890        900 
ASEHQLSATF YKFIGVIQAQ LLKSIYINFT SNYGNGGSGG SGSNGNGGGS SNGAGGGGLS 

       910        920        930        940        950        960 
SSVSIGNLNS PNINRIDFKR RSTIFETPSS LDILRDGNDS SFRTKFGLSQ DEVIIKRYQC 

       970        980        990       1000       1010       1020 
KHNNMNGTLY ITKHNLCFEG KFLGINKNKT IPFDRTDKIL TVDKNIMTVT TKEKVKKFTF 

      1030       1040       1050       1060       1070       1080 
KSHDDLNEGY GISVQIWANH LNINVKQQLQ LQQQQLQQQQ QQPSQQPSQQ QSQSSQLQQS 

      1090       1100       1110       1120       1130       1140 
VSASSTTPPV PNSPKVGRGE SFSNIKDLPS KDEWNQILKG TKPLTFKKGE ILICEGVEYQ 

      1150       1160       1170       1180       1190       1200 
KMFQIVKGEC SVVKSLLPTP LDQNIFNNIN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN 

      1210       1220       1230       1240       1250       1260 
NSIFKNNSSI SNTNSNTSID GGVGGGLSPQ TLVDPKNSVI VSKLTHGSIF GEMSFLLPGG 

      1270       1280       1290       1300       1310 
SATSLVVSSD EVEVYIIESY FLHILLKSKP YLASKFYKYL ACVLETRVRN LT 

« Hide

References

« Hide 'large scale' references
[1]"Identification of four candidate cGMP targets in Dictyostelium."
Goldberg J.M., Bosgraaf L., Van Haastert P.J.M., Smith J.L.
Proc. Natl. Acad. Sci. U.S.A. 99:6749-6754(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium."
Bosgraaf L., Russcher H., Smith J.L., Wessels D., Soll D.R., Van Haastert P.J.M.
EMBO J. 21:4560-4570(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles."
Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R., Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.
Genome Biol. 6:R68.1-R68.12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium."
Bosgraaf L., Waijer A., Engel R., Visser A.J.W.G., Wessels D., Soll D., van Haastert P.J.M.
J. Cell Sci. 118:1899-1910(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF481924 Genomic DNA. Translation: AAM34042.1.
AAFI02000047 Genomic DNA. Translation: EAL66046.1.
RefSeqXP_640199.1. XM_635107.1.

3D structure databases

ProteinModelPortalQ54S40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0215332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0215332; DDB0215332; DDB_G0282373.
GeneID8623739.
KEGGddi:DDB_G0282373.

Organism-specific databases

dictyBaseDDB_G0282373. gbpD.

Phylogenomic databases

eggNOGKOG3417.
OMAEAFINIA.

Family and domain databases

Gene3D1.10.840.10. 1 hit.
2.60.120.10. 3 hits.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR004182. GRAM.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF02893. GRAM. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTSM00568. GRAM. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMSSF48366. SSF48366. 2 hits.
SSF51206. SSF51206. 4 hits.
PROSITEPS50042. CNMP_BINDING_3. 2 hits.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGBPD_DICDI
AccessionPrimary (citable) accession number: Q54S40
Secondary accession number(s): Q8MVR0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 24, 2005
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase